FastQCFastQC Report
Thu 26 May 2016
SRR1544822_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544822_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2102551
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT86430.41107207387597255RNA PCR Primer, Index 41 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCTT84700.4028439738203734RNA PCR Primer, Index 41 (96% over 28bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT57710.27447610069862755Illumina PCR Primer Index 11 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42360.2014695481821844No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC39020.18558408333495835No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG24220.11519340077838777No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA24180.11500315569039705No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC23750.11295802099449669No Hit
TCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT23460.11157874410656389Illumina PCR Primer Index 11 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC22760.10824945506672609RNA PCR Primer, Index 41 (96% over 26bp)
ACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC21980.10453967585090683Illumina PCR Primer Index 11 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT700.046.0000043
CGCGAAC206.312655E-446.041
CCCGTCG206.312655E-446.018
CAAATCG206.312655E-446.044
CCGAATA206.312655E-446.024
TTCGCGA253.4181307E-546.041
TCGGTAA301.8621449E-646.036
ATAGCGG3300.044.6060641
GCGATTG1300.044.230778
ATAACGG1250.044.161
CTAACGG1600.043.1251
TATCCGG1300.042.461541
ATTACGG1800.042.1666681
CATACGA10850.041.54838617
CATGCGG4100.041.5121961
CGTAGGG8250.041.2606052
CGTTTTT32100.041.1993751
ACACGTG3350.041.1940341
GGCGATA3350.041.194037
CGAAGGG10900.040.935782
Produced by FastQC (version 0.11.5)