##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544822_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2102551
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.877992020169785	34.0	33.0	34.0	31.0	34.0
2	33.21983580897681	34.0	33.0	34.0	31.0	34.0
3	33.28768576838326	34.0	34.0	34.0	31.0	34.0
4	36.573312609301745	37.0	37.0	37.0	35.0	37.0
5	36.57198232052397	37.0	37.0	37.0	35.0	37.0
6	36.66424833452316	37.0	37.0	37.0	35.0	37.0
7	36.63028387896417	37.0	37.0	37.0	35.0	37.0
8	36.51845876746866	37.0	37.0	37.0	35.0	37.0
9	38.320280934921435	39.0	39.0	39.0	37.0	39.0
10	38.15909102799409	39.0	39.0	39.0	35.0	39.0
11	37.655611683141096	39.0	37.0	39.0	35.0	39.0
12	37.59048080165475	39.0	37.0	39.0	35.0	39.0
13	37.57435134748218	39.0	37.0	39.0	35.0	39.0
14	38.93511025416268	41.0	37.0	41.0	35.0	41.0
15	38.99204727970927	41.0	38.0	41.0	35.0	41.0
16	38.97095052628926	41.0	37.0	41.0	35.0	41.0
17	39.007961281319695	41.0	37.0	41.0	35.0	41.0
18	38.99330147045185	41.0	37.0	41.0	35.0	41.0
19	38.945252219803464	41.0	37.0	41.0	35.0	41.0
20	38.87263234042837	41.0	36.0	41.0	35.0	41.0
21	38.81464468638335	41.0	36.0	41.0	35.0	41.0
22	38.78741443132652	41.0	36.0	41.0	35.0	41.0
23	38.75158367145434	41.0	35.0	41.0	35.0	41.0
24	38.69393988540587	41.0	35.0	41.0	35.0	41.0
25	38.64039207610184	41.0	35.0	41.0	35.0	41.0
26	38.56863162891174	40.0	35.0	41.0	35.0	41.0
27	38.54080495550405	40.0	36.0	41.0	35.0	41.0
28	38.56471828745177	40.0	36.0	41.0	35.0	41.0
29	38.544609857263865	40.0	36.0	41.0	35.0	41.0
30	38.48723954852938	40.0	35.0	41.0	35.0	41.0
31	38.43652543981097	40.0	35.0	41.0	35.0	41.0
32	38.34169301957479	40.0	35.0	41.0	35.0	41.0
33	38.260293329388915	40.0	35.0	41.0	35.0	41.0
34	38.169300055028394	40.0	35.0	41.0	35.0	41.0
35	38.10861044512119	40.0	35.0	41.0	35.0	41.0
36	38.04779241977959	40.0	35.0	41.0	35.0	41.0
37	37.97733277337863	40.0	35.0	41.0	35.0	41.0
38	37.91615090430624	40.0	35.0	41.0	35.0	41.0
39	37.81075607678482	40.0	35.0	41.0	35.0	41.0
40	37.712811722521835	40.0	35.0	41.0	34.0	41.0
41	37.674046907780124	40.0	35.0	41.0	34.0	41.0
42	37.603431260407	40.0	35.0	41.0	34.0	41.0
43	37.62465119752149	40.0	35.0	41.0	34.0	41.0
44	37.572184455929964	40.0	35.0	41.0	34.0	41.0
45	37.54418941561941	40.0	35.0	41.0	35.0	41.0
46	37.488650215856836	39.0	35.0	41.0	35.0	41.0
47	37.39517376748531	39.0	35.0	41.0	34.0	41.0
48	37.35308489544368	39.0	35.0	41.0	34.0	41.0
49	37.28835210180395	39.0	35.0	41.0	34.0	41.0
50	37.19724325355247	39.0	35.0	41.0	34.0	41.0
51	37.113576317530466	39.0	35.0	41.0	34.0	41.0
52	36.84521707202346	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	1.0
12	1.0
13	6.0
14	11.0
15	25.0
16	42.0
17	72.0
18	138.0
19	271.0
20	513.0
21	849.0
22	1380.0
23	2234.0
24	3669.0
25	6159.0
26	8827.0
27	10530.0
28	11023.0
29	12417.0
30	14411.0
31	17874.0
32	22743.0
33	32533.0
34	97809.0
35	331397.0
36	85470.0
37	134687.0
38	226875.0
39	1069884.0
40	10698.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.89886856490045	22.10119992333123	24.197938599349076	19.801992912419248
2	28.24002842261615	24.131685747456306	25.42530478452128	22.20298104540627
3	28.59916358747065	24.14571632269562	24.88686362423551	22.36825646559822
4	26.545372740066707	26.500189531668912	22.796973771385332	24.15746395687905
5	25.164716575245976	36.08326266521003	19.896497159878642	18.855523599665357
6	86.89568053283844	3.15640381612622	7.124535861436892	2.823379789598445
7	87.36748835105546	3.1422305570709104	5.496846449860194	3.9934346420134403
8	77.26523637238763	5.683239074819112	10.513514297631781	6.538010255161468
9	47.367602498108255	24.460096330600305	14.101679340952966	14.070621830338478
10	37.494976340645245	22.900704905612276	24.310611252711585	15.29370750103089
11	36.65732721822206	18.74532413244673	25.366851981236128	19.23049666809509
12	22.871454723333702	28.742988873991642	27.947811967462382	20.437744435212274
13	16.546661650537846	31.20604446693564	30.030187139336928	22.217106743189582
14	15.9790654305175	25.988905857693823	37.724649723122056	20.307378988666624
15	19.00824284404992	19.67181771096159	40.62650561151668	20.693433833471815
16	19.122009406668376	22.385473646061378	28.27522376389443	30.217293183375816
17	20.351991461800452	24.401595966043153	29.519807129529795	25.7266054426266
18	23.831764366238918	27.93121308353519	26.670506446692617	21.566516103533278
19	27.703775080842274	23.941345536921578	25.108308906656724	23.24657047557943
20	24.012069148382132	27.688317667443023	26.24364403051341	22.055969153661433
21	21.413226123884748	25.211183937987713	26.16312279702133	27.21246714110621
22	21.18659666281579	27.66448947017219	24.786128850144422	26.36278501686761
23	21.781397930418812	22.46618512464145	33.28765865845823	22.46475828648152
24	21.94710140205874	22.236701987252626	30.077653288790618	25.738543321898018
25	19.597812371733195	29.331416931146975	27.42121356390404	23.649557133215794
26	19.590012323125574	27.506490924595884	28.49614587232367	24.407350879954873
27	21.544685479686343	26.11470542212769	31.187543132128543	21.153065966057422
28	19.88132511411138	25.8640575186999	28.573242694231908	25.681374672956803
29	21.34977938703984	26.25058797622507	28.58413422551938	23.815498411215707
30	25.037870662828155	25.258031790905427	23.413082488843315	26.2910150574231
31	27.773119415414893	26.362119159059638	24.228948548691566	21.635812876833903
32	23.44789733994562	27.98957076427635	25.150971367638647	23.41156052813939
33	19.518765537673048	24.813666826631078	26.87949067585043	28.788076959845444
34	21.32656948630497	26.200030344091534	27.171849814820188	25.30155035478331
35	24.192421491797344	25.226879157746946	26.107048057336062	24.473651293119644
36	21.434010399747734	28.61281367253398	24.9138308654582	25.039345062260082
37	21.647560511017332	28.05491995200116	26.94398376067929	23.353535776302216
38	20.875688627766937	26.94826427515908	25.520284644700652	26.65576245237333
39	24.765106767921445	23.78153966300936	27.884507914433463	23.568845654635727
40	17.31691645054032	22.234989781460712	32.06152906635796	28.386564701641003
41	20.579191658133382	25.800705904398992	25.509726042317165	28.11037639515046
42	21.84784102739957	23.525089284397858	27.698067728202552	26.929001960000022
43	22.643826475552792	26.361833791427653	26.788125472342884	24.206214260676674
44	19.4355808729491	28.054587023097184	25.86015749439609	26.64967460955763
45	20.86260927796757	25.72123101889086	26.532388512811345	26.883771190330226
46	22.331396479800013	24.697189271508755	27.896350671160892	25.075063577530344
47	21.516576767935714	21.91590120762826	31.062314302958644	25.505207721477387
48	19.746964520717928	22.26376435101931	28.583040316263435	29.40623081199933
49	19.715098468479482	25.051758554251478	30.498903474874094	24.734239502394946
50	19.391111083631266	24.78636665650441	28.534290012465807	27.28823224739852
51	20.472749531402567	22.582662679763775	29.219885748312407	27.724702040521255
52	21.488991230177056	20.66251900667332	31.656735080385683	26.19175468276394
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	242.0
1	797.0
2	1352.0
3	4740.5
4	8129.0
5	5405.5
6	2682.0
7	2909.5
8	3137.0
9	3183.5
10	3230.0
11	3085.0
12	2940.0
13	2816.0
14	2696.0
15	2700.0
16	2697.5
17	2695.0
18	3025.0
19	3355.0
20	3549.0
21	3743.0
22	5189.0
23	6635.0
24	7127.0
25	7619.0
26	9696.0
27	11773.0
28	15454.0
29	19135.0
30	21319.5
31	23504.0
32	24993.0
33	26482.0
34	31792.5
35	37103.0
36	41519.5
37	45936.0
38	49713.0
39	59815.5
40	66141.0
41	84274.0
42	102407.0
43	122669.5
44	142932.0
45	160050.0
46	177168.0
47	192480.5
48	207793.0
49	212173.0
50	216553.0
51	195747.0
52	174941.0
53	156954.5
54	138968.0
55	125119.0
56	111270.0
57	102947.0
58	94624.0
59	93394.0
60	92164.0
61	92970.5
62	93777.0
63	86679.0
64	64210.5
65	48840.0
66	38906.0
67	28972.0
68	24110.5
69	19249.0
70	16926.0
71	14603.0
72	11983.0
73	9363.0
74	7788.0
75	6213.0
76	5536.5
77	4860.0
78	3432.0
79	2004.0
80	1505.0
81	1006.0
82	677.0
83	348.0
84	243.5
85	139.0
86	87.5
87	36.0
88	26.0
89	10.0
90	4.0
91	4.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2102551.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.640130658866916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.15214443102829	14.06669165738393
2	12.99625790888554	5.364889226314601
3	5.211964170044537	3.2272686437715628
4	2.6875102605907064	2.218822517025506
5	1.569579463504764	1.6198162603106283
6	1.0102102824115466	1.2510523333143102
7	0.6763322120040294	0.977170965719455
8	0.4665611913700003	0.7703907160186734
9	0.36749998217053514	0.6826722884217996
>10	4.247728481401546	22.72233581323615
>50	2.2900590312847293	32.5723238029575
>100	0.30772515705968834	9.706170299463402
>500	0.009486261380435733	1.3428855129026225
>1k	0.006247050177360116	2.3846724070694325
>5k	6.941166863733462E-4	1.0928375560904555
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	8643	0.41107207387597255	RNA PCR Primer, Index 41 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCTT	8470	0.4028439738203734	RNA PCR Primer, Index 41 (96% over 28bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	5771	0.27447610069862755	Illumina PCR Primer Index 11 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4236	0.2014695481821844	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3902	0.18558408333495835	No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG	2422	0.11519340077838777	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	2418	0.11500315569039705	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2375	0.11295802099449669	No Hit
TCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	2346	0.11157874410656389	Illumina PCR Primer Index 11 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	2276	0.10824945506672609	RNA PCR Primer, Index 41 (96% over 26bp)
ACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	2198	0.10453967585090683	Illumina PCR Primer Index 11 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.41173793168394013	0.0
2	0.0	0.0	0.0	1.3060325290563701	0.0
3	0.0	0.0	0.0	2.0034234603583934	0.0
4	0.0	0.0	0.0	2.463008031672002	0.0
5	0.0	0.0	0.0	4.6779364686040905	0.0
6	0.0	0.0	0.0	5.036786265826608	0.0
7	0.0	0.0	0.0	6.30438928710885	0.0
8	0.0	0.0	0.0	7.876717378080246	0.0
9	0.0	0.0	0.0	8.974431535786765	0.0
10	4.7561271997682814E-5	0.0	0.0	9.875432272510869	0.0
11	4.7561271997682814E-5	0.0	0.0	10.74547062116448	0.0
12	9.512254399536563E-5	0.0	0.0	11.496605789823885	0.0
13	9.512254399536563E-5	0.0	0.0	11.824635882791904	0.0
14	9.512254399536563E-5	0.0	0.0	12.056306838692617	0.0
15	9.512254399536563E-5	0.0	0.0	12.809392019503926	0.0
16	9.512254399536563E-5	0.0	0.0	13.632487392695825	0.0
17	9.512254399536563E-5	0.0	0.0	14.627374080343355	0.0
18	9.512254399536563E-5	0.0	0.0	15.159584713997425	0.0
19	9.512254399536563E-5	0.0	0.0	15.812553417253612	0.0
20	9.512254399536563E-5	0.0	0.0	16.563736147185015	0.0
21	9.512254399536563E-5	0.0	0.0	17.286239430101816	0.0
22	9.512254399536563E-5	0.0	0.0	18.055400320848342	0.0
23	9.512254399536563E-5	0.0	0.0	18.66313825443473	0.0
24	9.512254399536563E-5	0.0	0.0	19.18445735680133	0.0
25	9.512254399536563E-5	0.0	0.0	19.668726228281738	0.0
26	9.512254399536563E-5	0.0	0.0	20.10733627864437	0.0
27	9.512254399536563E-5	0.0	0.0	20.572628202597702	0.0
28	1.4268381599304844E-4	0.0	0.0	21.0365408496631	0.0
29	1.4268381599304844E-4	0.0	0.0	21.58682476667629	0.0
30	1.4268381599304844E-4	0.0	0.0	22.152423413272732	0.0
31	1.4268381599304844E-4	0.0	0.0	22.69114994119049	0.0
32	1.9024508799073126E-4	0.0	0.0	23.176607844470837	0.0
33	1.9024508799073126E-4	0.0	0.0	23.648035172511868	0.0
34	1.9024508799073126E-4	0.0	0.0	24.242455949938908	0.0
35	1.9024508799073126E-4	0.0	0.0	24.746320065482358	0.0
36	1.9024508799073126E-4	0.0	0.0	25.288233198623956	0.0
37	1.9024508799073126E-4	0.0	0.0	25.758091004689067	0.0
38	1.9024508799073126E-4	0.0	0.0	26.25268067219297	0.0
39	1.9024508799073126E-4	0.0	0.0	26.77732906359941	0.0
40	1.9024508799073126E-4	0.0	0.0	27.361999780266924	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	70	0.0	46.000004	3
CGCGAAC	20	6.312655E-4	46.0	41
CCCGTCG	20	6.312655E-4	46.0	18
CAAATCG	20	6.312655E-4	46.0	44
CCGAATA	20	6.312655E-4	46.0	24
TTCGCGA	25	3.4181307E-5	46.0	41
TCGGTAA	30	1.8621449E-6	46.0	36
ATAGCGG	330	0.0	44.606064	1
GCGATTG	130	0.0	44.23077	8
ATAACGG	125	0.0	44.16	1
CTAACGG	160	0.0	43.125	1
TATCCGG	130	0.0	42.46154	1
ATTACGG	180	0.0	42.166668	1
CATACGA	1085	0.0	41.548386	17
CATGCGG	410	0.0	41.512196	1
CGTAGGG	825	0.0	41.260605	2
CGTTTTT	3210	0.0	41.199375	1
ACACGTG	335	0.0	41.19403	41
GGCGATA	335	0.0	41.19403	7
CGAAGGG	1090	0.0	40.93578	2
>>END_MODULE