FastQCFastQC Report
Thu 26 May 2016
SRR1544821_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544821_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1525261
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT105750.6933239622595739RNA PCR Primer, Index 41 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCTT95740.6276958500872966RNA PCR Primer, Index 41 (96% over 28bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT64650.42386188331046293Illumina PCR Primer Index 11 (96% over 26bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA64030.4197970052338584No Hit
TGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCTTG45120.29581822389741824Illumina PCR Primer Index 11 (96% over 28bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC26500.1737407565000351No Hit
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC23210.15217067767418166RNA PCR Primer, Index 41 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT22950.15046605138399263Illumina PCR Primer Index 11 (96% over 26bp)
ACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC21540.14122173188719833Illumina PCR Primer Index 11 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC19750.12948603550474314Illumina PCR Primer Index 11 (96% over 25bp)
AGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC18370.12043840365681677RNA PCR Primer, Index 41 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17720.1161768379313442No Hit
ACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT16320.10699808098417254Illumina PCR Primer Index 11 (96% over 26bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC15510.1016875144647375No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGCG206.3121074E-446.044
GGGCGTA253.4176846E-546.06
TACGAGT253.4176846E-546.017
ATAGCGG1850.044.7567561
GCTACGA2350.044.04255310
CGAATAT2400.043.12514
ACGGGAT5400.043.0185174
GTTACGG1500.042.933331
CTACGAA2450.042.244911
GTCCCCC7900.042.215199
CTCACGA1150.042.023
TACGGGA3650.041.5890433
CGTTTTT17300.041.479771
TCAACGG1000.041.41
GCGTAGG2450.041.306121
GGGCGAT12700.041.291346
GTCGGCG7950.041.081763
CATGCGG3700.041.0270271
CGAAGGG7750.040.9548382
ACATACG5350.040.8411216
Produced by FastQC (version 0.11.5)