##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544821_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1525261
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.875087607956935	34.0	33.0	34.0	31.0	34.0
2	33.24015693051878	34.0	33.0	34.0	31.0	34.0
3	33.34675507995025	34.0	34.0	34.0	31.0	34.0
4	36.578612447312295	37.0	37.0	37.0	35.0	37.0
5	36.58563681887887	37.0	37.0	37.0	35.0	37.0
6	36.50903484715075	37.0	37.0	37.0	35.0	37.0
7	36.37225825612797	37.0	37.0	37.0	35.0	37.0
8	36.40967480319762	37.0	37.0	37.0	35.0	37.0
9	38.268131814817266	39.0	39.0	39.0	37.0	39.0
10	38.17492153801874	39.0	39.0	39.0	35.0	39.0
11	37.752543335206234	39.0	37.0	39.0	35.0	39.0
12	37.7285894020761	39.0	37.0	39.0	35.0	39.0
13	37.719321480061446	39.0	37.0	39.0	35.0	39.0
14	39.06051882268019	41.0	38.0	41.0	35.0	41.0
15	39.11206868857199	41.0	38.0	41.0	35.0	41.0
16	39.10488106625686	41.0	38.0	41.0	35.0	41.0
17	39.15179107051187	41.0	38.0	41.0	35.0	41.0
18	39.1557294128677	41.0	38.0	41.0	35.0	41.0
19	39.11267317527951	41.0	38.0	41.0	35.0	41.0
20	39.05792385696612	41.0	38.0	41.0	35.0	41.0
21	38.999838060502434	41.0	38.0	41.0	35.0	41.0
22	38.98464590650387	41.0	37.0	41.0	35.0	41.0
23	38.9604205444183	41.0	37.0	41.0	35.0	41.0
24	38.87979958839831	41.0	37.0	41.0	35.0	41.0
25	38.8257498224894	41.0	37.0	41.0	35.0	41.0
26	38.754466284786666	41.0	36.0	41.0	35.0	41.0
27	38.72924699444882	41.0	36.0	41.0	35.0	41.0
28	38.79215032705878	41.0	36.0	41.0	35.0	41.0
29	38.80851736194658	41.0	36.0	41.0	35.0	41.0
30	38.77365513181023	41.0	36.0	41.0	35.0	41.0
31	38.76075832267396	41.0	36.0	41.0	35.0	41.0
32	38.702405686633305	41.0	36.0	41.0	35.0	41.0
33	38.63936073891616	41.0	36.0	41.0	35.0	41.0
34	38.577848643609194	41.0	36.0	41.0	35.0	41.0
35	38.500205538593065	41.0	36.0	41.0	35.0	41.0
36	38.47263124147277	40.0	36.0	41.0	35.0	41.0
37	38.41798879011527	40.0	35.0	41.0	35.0	41.0
38	38.36829565562878	40.0	35.0	41.0	35.0	41.0
39	38.3083485383813	40.0	35.0	41.0	35.0	41.0
40	38.236825041746954	40.0	35.0	41.0	35.0	41.0
41	38.21350247596968	40.0	35.0	41.0	35.0	41.0
42	38.148638167500515	40.0	35.0	41.0	35.0	41.0
43	38.07867702642368	40.0	35.0	41.0	35.0	41.0
44	37.923321320088824	40.0	35.0	41.0	35.0	41.0
45	37.905791861196214	40.0	35.0	41.0	35.0	41.0
46	37.85746242774187	40.0	35.0	41.0	35.0	41.0
47	37.76631868250745	40.0	35.0	41.0	35.0	41.0
48	37.70220047585298	40.0	35.0	41.0	35.0	41.0
49	37.65186482838019	39.0	35.0	41.0	35.0	41.0
50	37.56672595706571	39.0	35.0	41.0	35.0	41.0
51	37.48578636705456	39.0	35.0	41.0	35.0	41.0
52	37.053235479042606	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	0.0
13	5.0
14	11.0
15	22.0
16	20.0
17	45.0
18	116.0
19	203.0
20	300.0
21	525.0
22	840.0
23	1225.0
24	2104.0
25	3326.0
26	4824.0
27	5826.0
28	6181.0
29	6596.0
30	8094.0
31	9945.0
32	13429.0
33	19703.0
34	61691.0
35	226070.0
36	57204.0
37	94888.0
38	165887.0
39	825196.0
40	10983.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.6741711746383	21.60482697715342	23.005570849841437	21.715430998366834
2	27.748234564445035	24.20366088164583	25.70668233174519	22.341422222163946
3	28.641852115801818	23.914136662512185	24.73563540928405	22.708375812401943
4	26.781908145556727	25.77034356742879	23.175115603165622	24.27263268384886
5	24.915014545051633	35.1868303195322	20.014082835658947	19.88407229975722
6	84.55523349774235	3.1829962216302654	8.360405202781687	3.9013650778456936
7	85.23124894690154	3.15323082410158	6.472007086000363	5.14351314299651
8	73.58668450842184	5.7535726672353125	12.445607669769306	8.214135154573544
9	45.84015457026699	23.28545737418055	15.262502614306667	15.611885441245793
10	35.765288694852885	22.087367342376158	26.445244453244392	15.702099509526565
11	34.360479944088254	18.589605320007525	26.0467552766379	21.003159459266314
12	22.08867859336861	27.066384048369425	29.469513742238213	21.37542361602375
13	16.30160346327612	29.80998006242866	31.085237215138918	22.803179259156302
14	15.708065701542228	25.6427588458631	36.690441832578166	21.958733620016506
15	18.319618740661433	19.574289252790177	40.34752085052985	21.758571156018544
16	18.266578638016707	23.498732348103047	28.382880044792337	29.851808969087912
17	19.334133633522395	24.644569027858182	29.764479652990538	26.256817685628885
18	22.71886582034157	28.080308878283784	27.384100163840813	21.816725137533837
19	26.09546825100753	23.65470565365534	25.60099550175347	24.648830593583657
20	22.769217858451768	28.200419469192482	26.69726689399388	22.333095778361866
21	20.62040529456926	25.044631705655622	26.580893368413665	27.75406963136145
22	20.368251728720526	27.589573194358213	24.711573953572536	27.330601123348725
23	20.759725712517398	22.425801223528303	33.56874659484508	23.245726469109222
24	22.03065573695256	22.18997273253561	29.59663952595654	26.182732004555287
25	19.146690304151225	28.719084799257306	27.409997370941756	24.72422752564971
26	19.3541302111573	26.396006978477782	28.446803530674426	25.803059279690494
27	22.446912364506797	25.257775554478872	30.32562951521084	21.96968256580349
28	20.226767746634838	25.260791431761515	27.569904429471414	26.942536392132233
29	21.442625229386973	25.69553669830934	28.29456729045062	24.567270781853072
30	24.31111790047736	25.084493735826197	22.681036229209294	27.923352134487146
31	27.543613847072727	25.56119903413252	23.748656787264604	23.146530331530144
32	22.208067996231463	27.853134643841283	25.480950473394387	24.457846886532863
33	18.998584504553648	23.673063167549685	26.65235654750236	30.675995780394306
34	21.481044883465845	25.836496179998047	26.989151364913937	25.69330757162217
35	23.261199230820168	24.48767784661117	26.815738421162017	25.435384501406645
36	21.38211099608526	27.992520624339047	23.94954043930842	26.675827940267272
37	21.24803558210693	27.64556361173596	27.607012832557835	23.499387973599273
38	20.333372452321274	26.252752807552284	25.197851384123766	28.21602335600268
39	24.85463143684917	22.876347064535185	27.909977374364125	24.359044124251522
40	17.062456851647028	21.282455920658823	32.32338596476275	29.331701262931393
41	20.505933082928102	25.1878203140315	25.41014291980192	28.89610368323848
42	21.457835740899426	22.081794525658232	28.37684829022705	28.083521443215293
43	23.404191151547177	25.087116237811102	26.528902266562902	24.97979034407882
44	19.370717536211835	26.499333556683087	26.631442094172737	27.498506812932344
45	20.415850139746574	24.25080035482452	27.01386844612168	28.31948105930723
46	22.67762697662892	22.96020156550256	28.110926588957565	26.251244868910963
47	20.531436914731316	20.836433895575905	32.18773704959348	26.4443921400993
48	19.550031109429796	20.756840960334003	28.35855633888233	31.33457159135387
49	19.75911008017644	23.212289568801665	31.218329190872907	25.810271160148986
50	19.43155958226166	22.78803431019347	29.598934215193335	28.18147189235154
51	20.31350699978561	20.883114430907234	29.44105959570198	29.362318973605174
52	20.74569532689815	19.621625413617735	33.47518883653355	26.157490422950563
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	151.0
1	411.5
2	672.0
3	2288.5
4	3905.0
5	2734.0
6	1563.0
7	1650.5
8	1738.0
9	1779.0
10	1820.0
11	1791.5
12	1763.0
13	1716.0
14	1660.0
15	1651.0
16	1642.0
17	1633.0
18	1885.5
19	2138.0
20	2242.5
21	2347.0
22	3243.5
23	4140.0
24	4370.0
25	4600.0
26	5893.0
27	7186.0
28	9238.5
29	11291.0
30	12766.5
31	14242.0
32	15313.5
33	16385.0
34	19922.0
35	23459.0
36	26267.5
37	29076.0
38	31793.0
39	38643.5
40	42777.0
41	54874.0
42	66971.0
43	81972.0
44	96973.0
45	112258.5
46	127544.0
47	148850.5
48	170157.0
49	175739.5
50	181322.0
51	161710.5
52	142099.0
53	124596.5
54	107094.0
55	94262.0
56	81430.0
57	73588.0
58	65746.0
59	64125.0
60	62504.0
61	62733.0
62	62962.0
63	60748.5
64	45851.0
65	33167.0
66	26521.0
67	19875.0
68	16563.5
69	13252.0
70	11700.5
71	10149.0
72	8365.5
73	6582.0
74	5497.5
75	4413.0
76	3890.5
77	3368.0
78	2357.5
79	1347.0
80	1020.5
81	694.0
82	451.0
83	208.0
84	161.0
85	114.0
86	70.0
87	26.0
88	16.0
89	4.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1525261.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.082802423676263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.71401654259843	16.548260841326204
2	12.8137148373518	6.1718032548254484
3	4.9874333954639125	3.6033411919260674
4	2.438174435854098	2.3487229285253033
5	1.394336342615386	1.6789763325678857
6	0.9474685655177111	1.3690618959604202
7	0.639576403267469	1.0781954508315166
8	0.45070229510464244	0.8683339459922029
9	0.3723654389240475	0.8070842965511995
>10	6.265765186482882	40.97531322650976
>50	0.8097111169314983	12.13302480021689
>100	0.15118074259318884	6.3151106484786155
>500	0.008186682786803137	1.4130332785172413
>1k	0.006276456803215738	2.5198797729494715
>5k	8.186682786803138E-4	1.474875254010694
>10k+	2.728894262267712E-4	0.694982880811117
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	10575	0.6933239622595739	RNA PCR Primer, Index 41 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCTT	9574	0.6276958500872966	RNA PCR Primer, Index 41 (96% over 28bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	6465	0.42386188331046293	Illumina PCR Primer Index 11 (96% over 26bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	6403	0.4197970052338584	No Hit
TGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCTTG	4512	0.29581822389741824	Illumina PCR Primer Index 11 (96% over 28bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2650	0.1737407565000351	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	2321	0.15217067767418166	RNA PCR Primer, Index 41 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	2295	0.15046605138399263	Illumina PCR Primer Index 11 (96% over 26bp)
ACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	2154	0.14122173188719833	Illumina PCR Primer Index 11 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	1975	0.12948603550474314	Illumina PCR Primer Index 11 (96% over 25bp)
AGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	1837	0.12043840365681677	RNA PCR Primer, Index 41 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1772	0.1161768379313442	No Hit
ACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	1632	0.10699808098417254	Illumina PCR Primer Index 11 (96% over 26bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1551	0.1016875144647375	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6405461098133368	0.0
2	0.0	0.0	0.0	2.0415522326998463	0.0
3	0.0	0.0	0.0	3.0408566140483497	0.0
4	0.0	0.0	0.0	3.708283369206975	0.0
5	0.0	0.0	0.0	6.8114899679464695	0.0
6	0.0	0.0	0.0	7.318944102025817	0.0
7	6.556254962265474E-5	0.0	0.0	9.134436663626749	0.0
8	6.556254962265474E-5	0.0	0.0	11.31019543540417	0.0
9	6.556254962265474E-5	0.0	0.0	12.833606838436175	0.0
10	6.556254962265474E-5	0.0	0.0	14.068739710777368	0.0
11	6.556254962265474E-5	0.0	0.0	15.22185383354062	0.0
12	6.556254962265474E-5	0.0	0.0	16.235975351103843	0.0
13	6.556254962265474E-5	0.0	0.0	16.678260310858274	0.0
14	6.556254962265474E-5	0.0	0.0	16.987453294878712	0.0
15	6.556254962265474E-5	0.0	0.0	17.946764520957398	0.0
16	6.556254962265474E-5	0.0	0.0	18.998256691805533	0.0
17	6.556254962265474E-5	0.0	0.0	20.167630326875205	0.0
18	6.556254962265474E-5	0.0	0.0	20.823124698002506	0.0
19	6.556254962265474E-5	0.0	0.0	21.59722172139719	0.0
20	6.556254962265474E-5	0.0	0.0	22.49274058669303	0.0
21	6.556254962265474E-5	0.0	0.0	23.34800404652056	0.0
22	6.556254962265474E-5	0.0	0.0	24.247063289496026	0.0
23	6.556254962265474E-5	0.0	0.0	24.94681238161862	0.0
24	6.556254962265474E-5	0.0	0.0	25.553462653277045	0.0
25	6.556254962265474E-5	0.0	0.0	26.085830556213	0.0
26	1.3112509924530948E-4	0.0	0.0	26.576631802688194	0.0
27	1.3112509924530948E-4	0.0	0.0	27.086052813256224	0.0
28	1.3112509924530948E-4	0.0	0.0	27.59789963816029	0.0
29	1.9668764886796424E-4	0.0	0.0	28.21189291537645	0.0
30	1.9668764886796424E-4	0.0	0.0	28.81349487071393	0.0
31	1.9668764886796424E-4	0.0	0.0	29.378185110613856	0.0
32	1.9668764886796424E-4	0.0	0.0	29.895276939487733	0.0
33	1.9668764886796424E-4	0.0	0.0	30.388962938146324	0.0
34	1.9668764886796424E-4	0.0	0.0	31.01908460256966	0.0
35	1.9668764886796424E-4	0.0	0.0	31.52817780038957	0.0
36	1.9668764886796424E-4	0.0	0.0	32.0885409120144	0.0
37	1.9668764886796424E-4	0.0	0.0	32.57953884613846	0.0
38	1.9668764886796424E-4	0.0	0.0	33.10961205983763	0.0
39	1.9668764886796424E-4	0.0	0.0	33.656731536438684	0.0
40	1.9668764886796424E-4	0.0	0.0	34.25426861369956	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTCGCG	20	6.3121074E-4	46.0	44
GGGCGTA	25	3.4176846E-5	46.0	6
TACGAGT	25	3.4176846E-5	46.0	17
ATAGCGG	185	0.0	44.756756	1
GCTACGA	235	0.0	44.042553	10
CGAATAT	240	0.0	43.125	14
ACGGGAT	540	0.0	43.018517	4
GTTACGG	150	0.0	42.93333	1
CTACGAA	245	0.0	42.2449	11
GTCCCCC	790	0.0	42.21519	9
CTCACGA	115	0.0	42.0	23
TACGGGA	365	0.0	41.589043	3
CGTTTTT	1730	0.0	41.47977	1
TCAACGG	100	0.0	41.4	1
GCGTAGG	245	0.0	41.30612	1
GGGCGAT	1270	0.0	41.29134	6
GTCGGCG	795	0.0	41.08176	3
CATGCGG	370	0.0	41.027027	1
CGAAGGG	775	0.0	40.954838	2
ACATACG	535	0.0	40.84112	16
>>END_MODULE