FastQCFastQC Report
Thu 26 May 2016
SRR1544820_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544820_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1351497
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC45040.3332600812284452No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT42120.3116544098877023No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC38540.2851652648877504No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTT36750.27192069238777444Illumina Single End Adapter 1 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG32920.24358174675933428No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT29720.21990429871468453No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26260.194303058016407No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA23460.17358529097733846No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC23380.1729933547762222No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC21540.1593788221505486No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC14580.1078803726534354No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC13590.1005551621646219No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCA206.311851E-446.042
CGCGATA206.311851E-446.017
TCGATAG206.311851E-446.017
CTAACGT405.6134013E-946.013
CGTACGA206.311851E-446.026
TACCGAT206.311851E-446.014
CGTTTTT16550.042.94261
GCGATAA650.042.461548
CGGGATC2150.041.7209325
CACGACG508.731149E-1041.425
CGGTCTA508.731149E-1041.430
CATGCGG2450.041.306121
ACGGGAT3900.041.282054
CTAGCGG2850.041.1578941
TTAATCG451.5887053E-840.8888919
TACGGGA3250.040.3384633
TAGTAGG3550.040.1690141
CGGGTAT1500.039.866675
CCAACGG1100.039.727271
ATAGGGC4700.039.6382983
Produced by FastQC (version 0.11.5)