##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544820_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1351497
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83510285261455	33.0	31.0	34.0	31.0	34.0
2	33.198541321216396	34.0	33.0	34.0	31.0	34.0
3	33.27970983287421	34.0	34.0	34.0	31.0	34.0
4	36.55635639590765	37.0	37.0	37.0	35.0	37.0
5	36.53943072015698	37.0	37.0	37.0	35.0	37.0
6	36.63494628548935	37.0	37.0	37.0	35.0	37.0
7	36.637501230117415	37.0	37.0	37.0	35.0	37.0
8	36.54709925364244	37.0	37.0	37.0	35.0	37.0
9	38.32900627970317	39.0	39.0	39.0	37.0	39.0
10	38.116272548144764	39.0	39.0	39.0	35.0	39.0
11	37.464661038833235	39.0	35.0	39.0	35.0	39.0
12	37.36327346638579	39.0	35.0	39.0	35.0	39.0
13	37.303342145783525	39.0	35.0	39.0	35.0	39.0
14	38.6257157803532	41.0	35.0	41.0	35.0	41.0
15	38.684462488632974	41.0	35.0	41.0	35.0	41.0
16	38.65847057004196	41.0	35.0	41.0	35.0	41.0
17	38.71003931196296	41.0	36.0	41.0	35.0	41.0
18	38.69724609081633	41.0	36.0	41.0	35.0	41.0
19	38.63329922300974	41.0	35.0	41.0	35.0	41.0
20	38.56790211151042	41.0	35.0	41.0	35.0	41.0
21	38.52024384811805	40.0	35.0	41.0	35.0	41.0
22	38.481566736737115	40.0	35.0	41.0	35.0	41.0
23	38.438775668758424	40.0	35.0	41.0	35.0	41.0
24	38.38617251832598	40.0	35.0	41.0	35.0	41.0
25	38.33372919066783	40.0	35.0	41.0	35.0	41.0
26	38.261453040591284	40.0	35.0	41.0	35.0	41.0
27	38.23107857435126	40.0	35.0	41.0	35.0	41.0
28	38.264279535951616	40.0	35.0	41.0	35.0	41.0
29	38.25738199936811	40.0	35.0	41.0	35.0	41.0
30	38.19707627911863	40.0	35.0	41.0	35.0	41.0
31	38.148601883688976	40.0	35.0	41.0	35.0	41.0
32	38.0823301864525	40.0	35.0	41.0	35.0	41.0
33	37.98461113861148	40.0	35.0	41.0	35.0	41.0
34	37.908462245939134	40.0	35.0	41.0	35.0	41.0
35	37.820815732480355	40.0	35.0	41.0	35.0	41.0
36	37.77302206368198	40.0	35.0	41.0	34.0	41.0
37	37.695583490011444	40.0	35.0	41.0	34.0	41.0
38	37.61113713163995	40.0	35.0	41.0	34.0	41.0
39	37.515839842781745	40.0	35.0	41.0	34.0	41.0
40	37.41823104305818	39.0	35.0	41.0	34.0	41.0
41	37.370288650289275	39.0	35.0	41.0	34.0	41.0
42	37.294213009721815	39.0	35.0	41.0	34.0	41.0
43	37.30629960702836	39.0	35.0	41.0	34.0	41.0
44	37.256686474331794	39.0	35.0	41.0	34.0	41.0
45	37.22743372719288	39.0	35.0	41.0	34.0	41.0
46	37.17820831270806	39.0	35.0	41.0	34.0	41.0
47	37.080807430575135	38.0	35.0	41.0	34.0	41.0
48	37.04301452389461	38.0	35.0	41.0	34.0	41.0
49	36.98021305263719	38.0	35.0	41.0	34.0	41.0
50	36.883862117340996	37.0	35.0	41.0	34.0	41.0
51	36.78818302963307	37.0	35.0	41.0	34.0	41.0
52	36.508166869774776	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	0.0
12	4.0
13	3.0
14	8.0
15	15.0
16	30.0
17	79.0
18	125.0
19	209.0
20	388.0
21	638.0
22	992.0
23	1523.0
24	2438.0
25	4080.0
26	5594.0
27	6812.0
28	7546.0
29	8528.0
30	9834.0
31	12550.0
32	16118.0
33	23391.0
34	79474.0
35	245352.0
36	56723.0
37	89493.0
38	157563.0
39	617457.0
40	4527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.850093636907815	21.964458670644476	24.786958461617008	19.3984892308307
2	27.920224758175564	23.68025974160505	26.118444954002857	22.281070546216526
3	28.166914169990758	24.260875162874946	25.189179110275496	22.3830315568588
4	26.73435457126431	26.699208359323034	23.069677550153646	23.496759519259015
5	24.897132587049768	35.30995629291075	21.003820208257952	18.78909091178153
6	87.60160029951972	2.6848746242130024	7.217626084260638	2.4958989920066417
7	89.54966233739327	2.36212141055437	4.854172817253756	3.2340434347985973
8	81.10251077138906	4.153616323232682	9.428877755555506	5.314995149822752
9	52.36467413542169	18.855831718457384	13.84997524966759	14.92951889645334
10	42.62991334793936	20.982510505017768	21.551065226189923	14.83651092085295
11	38.27274496354783	19.067744878457	23.295649194929773	19.363860963065402
12	23.790877819188648	27.94138647736547	28.190739602085685	20.076996101360194
13	18.911991665538288	30.495147233031222	29.843277491551962	20.749583609878528
14	18.535890201754054	23.258431206284584	38.55894611678753	19.646732475173824
15	21.117989903048247	19.519244215858414	38.689764017234225	20.673001863859113
16	22.606339488729905	21.293720962754634	25.648891562467398	30.451047986048064
17	23.284920351284537	23.367791419440813	29.13902139627391	24.20826683300074
18	26.207827320371415	26.57497574911376	25.88300233000887	21.334194600505956
19	28.35751762675019	23.82964964036176	23.398868069999416	24.413964662888635
20	24.641934092343526	28.351820240814448	26.396432992452073	20.609812674389953
21	23.038156947444204	22.55077147785012	27.353075885481065	27.05799568922462
22	21.887728940574785	26.389551734114097	25.706087397900255	26.016631927410867
23	22.684993011453226	22.33930227000134	32.55145960368392	22.42424511486152
24	24.46176351112877	20.917619498970403	29.52807146445756	25.092545525443267
25	19.53863012644497	27.994216783315096	27.42026064430776	25.04689244593218
26	19.533598668735483	27.24504752877735	28.28789113109389	24.93346267139328
27	21.988506078814826	25.220625720959795	30.731255785251467	22.059612414973913
28	20.33944581453011	25.477156072118547	28.83927970243367	25.34411841091767
29	22.501196821006634	24.14056412999807	28.266877395954264	25.091361653041034
30	29.058074120771266	23.450514503546806	23.671676666688864	23.819734708993064
31	28.385708588328352	25.450666927118593	22.451548172138008	23.712076312415046
32	26.498985939295462	24.98636696936804	24.551145877497323	23.963501213839173
33	20.985914138174188	25.936720540260172	26.931247350160596	26.14611797140504
34	21.118063895073387	25.661174238640562	27.75226286110883	25.468499005177218
35	27.4255880701178	25.639790543375234	24.72820879365622	22.206412592850743
36	23.116958454217805	27.193919039405934	26.221367860971945	23.46775464540432
37	22.58998725117407	29.022483956679153	25.281595149674768	23.105933642472014
38	20.832306693984524	27.74708341934906	25.670645217858418	25.749964668807994
39	25.202719650876027	23.888769268448247	26.887814031403696	24.02069704927203
40	19.793458661025515	22.58517776954	29.846163180532404	27.775200388902082
41	21.382067440771234	25.516889789618475	24.62913347199439	28.471909297615905
42	22.09461064286491	21.872264607320623	27.837057721918733	28.196067027895733
43	22.674264167807994	25.088919916211434	27.5712043755924	24.665611540388177
44	21.212033767000595	27.31748572138895	25.02906036787355	26.44142014373691
45	22.798348793966987	25.980227851042216	25.505495017746988	25.71592833724381
46	23.807600016870182	23.984884909104498	27.216930559224327	24.990584514800997
47	21.806707673047	23.27833506104712	30.178609349484315	24.73634791642157
48	21.502674441748667	22.050659379932032	28.63454376887259	27.81212240944671
49	21.397531774025396	25.08877193216115	28.7910369020427	24.722659391770755
50	20.1614210020444	26.70586764158559	27.510160954852285	25.622550401517724
51	21.608483037698196	21.955579627627735	28.015822454655837	28.420114880018232
52	22.74078299840843	20.608406825912304	30.36802893384151	26.282781241837753
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	227.0
1	444.5
2	662.0
3	2715.0
4	4768.0
5	3107.0
6	1446.0
7	1569.5
8	1693.0
9	1720.5
10	1748.0
11	1738.0
12	1728.0
13	1717.5
14	1648.0
15	1589.0
16	1491.5
17	1394.0
18	1375.0
19	1356.0
20	1530.0
21	1704.0
22	1866.0
23	2028.0
24	2117.0
25	2206.0
26	2974.0
27	3742.0
28	5334.5
29	6927.0
30	8087.0
31	9247.0
32	9956.0
33	10665.0
34	13157.0
35	15649.0
36	17377.0
37	19105.0
38	21342.5
39	29278.5
40	34977.0
41	49804.5
42	64632.0
43	84641.5
44	104651.0
45	119912.0
46	135173.0
47	137621.5
48	140070.0
49	135816.5
50	131563.0
51	117249.5
52	102936.0
53	94339.5
54	85743.0
55	80852.5
56	75962.0
57	72732.5
58	69503.0
59	70818.0
60	72133.0
61	67403.5
62	62674.0
63	58077.0
64	45503.0
65	37526.0
66	30945.0
67	24364.0
68	19938.5
69	15513.0
70	13427.0
71	11341.0
72	8847.0
73	6353.0
74	5495.0
75	4637.0
76	3625.5
77	2614.0
78	2010.5
79	1407.0
80	1054.0
81	701.0
82	454.5
83	208.0
84	149.5
85	91.0
86	59.0
87	27.0
88	31.5
89	21.0
90	6.0
91	4.0
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1351497.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.145923352730872
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.40641028532222	15.592149657196682
2	11.984404172569176	5.308113924677301
3	4.36048837124258	2.897011237500374
4	2.069246214563645	1.8330147226261997
5	1.1612764472392667	1.2858769595946207
6	0.7685370856064532	1.0211978034943008
7	0.5699337374902692	0.8835196206616457
8	0.43479150666502997	0.7703087504817703
9	0.32560043189275867	0.6489649987481791
>10	6.050340952804574	36.53566058795807
>50	1.705343813386972	23.856844765126006
>100	0.1508855359584037	5.3282808700650754
>500	0.007376626202389426	1.1021750509257264
>1k	0.005364819056283219	2.936881050944109
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	4504	0.3332600812284452	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	4212	0.3116544098877023	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3854	0.2851652648877504	No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTT	3675	0.27192069238777444	Illumina Single End Adapter 1 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	3292	0.24358174675933428	No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	2972	0.21990429871468453	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2626	0.194303058016407	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2346	0.17358529097733846	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2338	0.1729933547762222	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2154	0.1593788221505486	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1458	0.1078803726534354	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1359	0.1005551621646219	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2809477194547972	0.0
2	0.0	0.0	0.0	0.9920850730708244	0.0
3	0.0	0.0	0.0	1.5863150269663935	0.0
4	0.0	0.0	0.0	1.9787687283064632	0.0
5	0.0	0.0	0.0	4.067045653819432	0.0
6	0.0	0.0	0.0	4.405781144908202	0.0
7	0.0	0.0	0.0	5.600382390785921	0.0
8	0.0	0.0	0.0	7.058469238185508	0.0
9	0.0	0.0	0.0	8.176636722094093	0.0
10	0.0	0.0	0.0	9.001573810374717	0.0
11	7.399202513953046E-5	0.0	0.0	9.802833450610693	0.0
12	7.399202513953046E-5	0.0	0.0	10.493844973388768	0.0
13	7.399202513953046E-5	0.0	0.0	10.804537486949657	0.0
14	7.399202513953046E-5	0.0	0.0	11.007571603932528	0.0
15	1.4798405027906093E-4	0.0	0.0	11.679641168274884	0.0
16	1.4798405027906093E-4	0.0	0.0	12.395292035424422	0.0
17	1.4798405027906093E-4	0.0	0.0	13.340244188481366	0.0
18	1.4798405027906093E-4	0.0	0.0	13.882679724779264	0.0
19	1.4798405027906093E-4	0.0	0.0	14.45848566441509	0.0
20	1.4798405027906093E-4	0.0	0.0	15.168661121704302	0.0
21	1.4798405027906093E-4	0.0	0.0	15.769698341912708	0.0
22	1.4798405027906093E-4	0.0	0.0	16.41720255390874	0.0
23	1.4798405027906093E-4	0.0	0.0	16.926563654969268	0.0
24	1.4798405027906093E-4	0.0	0.0	17.36888798125338	0.0
25	1.4798405027906093E-4	0.0	0.0	17.81639174929726	0.0
26	1.4798405027906093E-4	0.0	0.0	18.232152938556283	0.0
27	1.4798405027906093E-4	0.0	0.0	18.64539839896056	0.0
28	1.4798405027906093E-4	0.0	0.0	19.084319092088254	0.0
29	1.4798405027906093E-4	0.0	0.0	19.57510819483876	0.0
30	1.4798405027906093E-4	0.0	0.0	20.278476385815136	0.0
31	1.4798405027906093E-4	0.0	0.0	20.72716402626125	0.0
32	1.4798405027906093E-4	0.0	0.0	21.18413877352299	0.0
33	1.4798405027906093E-4	0.0	0.0	21.696829515714796	0.0
34	2.2197607541859138E-4	0.0	0.0	22.34425973568569	0.0
35	2.2197607541859138E-4	0.0	0.0	22.808115741285405	0.0
36	2.2197607541859138E-4	0.0	0.0	23.284994343309677	0.0
37	2.2197607541859138E-4	0.0	0.0	23.82165850164669	0.0
38	2.2197607541859138E-4	0.0	0.0	24.52029120301414	0.0
39	2.2197607541859138E-4	0.0	0.0	25.01840551625346	0.0
40	2.2197607541859138E-4	0.0	0.0	25.510452483431337	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTCA	20	6.311851E-4	46.0	42
CGCGATA	20	6.311851E-4	46.0	17
TCGATAG	20	6.311851E-4	46.0	17
CTAACGT	40	5.6134013E-9	46.0	13
CGTACGA	20	6.311851E-4	46.0	26
TACCGAT	20	6.311851E-4	46.0	14
CGTTTTT	1655	0.0	42.9426	1
GCGATAA	65	0.0	42.46154	8
CGGGATC	215	0.0	41.720932	5
CACGACG	50	8.731149E-10	41.4	25
CGGTCTA	50	8.731149E-10	41.4	30
CATGCGG	245	0.0	41.30612	1
ACGGGAT	390	0.0	41.28205	4
CTAGCGG	285	0.0	41.157894	1
TTAATCG	45	1.5887053E-8	40.88889	19
TACGGGA	325	0.0	40.338463	3
TAGTAGG	355	0.0	40.169014	1
CGGGTAT	150	0.0	39.86667	5
CCAACGG	110	0.0	39.72727	1
ATAGGGC	470	0.0	39.638298	3
>>END_MODULE