FastQCFastQC Report
Thu 26 May 2016
SRR1544819_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544819_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1012220
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA80730.7975538914465236No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT57790.5709233170654601No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTT48080.47499555432613466Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT37950.37491849597913496No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC32340.319495761790915No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG27410.27079093477702476No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC27130.26802473770524193No Hit
TGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTTG25330.250242042243781Illumina Single End Adapter 1 (95% over 22bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC17630.17417162276975362No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC16930.16725613009029658No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC13460.13297504495070242No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT12970.1281342000750825No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC12710.12556558850842703No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC12390.12240422042638952No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11820.11677303353026022No Hit
GGGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT10650.10521428148031062No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC10200.10076860761494538No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACG453.092282E-1046.025
CAATCGT206.3110964E-446.011
CCCGCTA206.3110964E-446.014
ACGGGTA950.043.5789454
TCGGCGT11000.043.0727274
CTAACGG750.042.9333341
GCGTAGG1500.042.9333341
AGTACGG700.042.7142871
GTCGGCG11150.042.2869953
CTTGCGG3700.042.270271
TAGACGG601.8189894E-1242.1666681
GTCCCCC11300.042.1327449
CACGAGG1100.041.818181
CGTTTTT10250.041.7365881
GCGTCCC11450.041.5807847
CGATAGT508.712959E-1041.3999989
TAGTAGG2000.041.3999981
CTAGCGG2900.041.2413831
TCCCCCA11600.040.8448310
ATTGCGG2500.040.481