##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544819_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1012220
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81044634565608	34.0	33.0	34.0	31.0	34.0
2	33.202251486830924	34.0	33.0	34.0	31.0	34.0
3	33.31504712414297	34.0	34.0	34.0	31.0	34.0
4	36.532652980577346	37.0	37.0	37.0	35.0	37.0
5	36.5396376281836	37.0	37.0	37.0	35.0	37.0
6	36.47640927861532	37.0	37.0	37.0	35.0	37.0
7	36.3949171128806	37.0	37.0	37.0	35.0	37.0
8	36.435620714864356	37.0	37.0	37.0	35.0	37.0
9	38.27118412993223	39.0	39.0	39.0	37.0	39.0
10	38.132130366916286	39.0	39.0	39.0	35.0	39.0
11	37.56859575981506	39.0	35.0	39.0	35.0	39.0
12	37.51044041809093	39.0	35.0	39.0	35.0	39.0
13	37.46140463535595	39.0	35.0	39.0	35.0	39.0
14	38.75123886111715	41.0	36.0	41.0	35.0	41.0
15	38.8107516152615	41.0	36.0	41.0	35.0	41.0
16	38.798039951789136	41.0	36.0	41.0	35.0	41.0
17	38.85892197348402	41.0	36.0	41.0	35.0	41.0
18	38.85953053683982	41.0	36.0	41.0	35.0	41.0
19	38.81282231135524	41.0	36.0	41.0	35.0	41.0
20	38.75851198356089	41.0	35.0	41.0	35.0	41.0
21	38.7070636818083	41.0	35.0	41.0	35.0	41.0
22	38.681633439370884	41.0	35.0	41.0	35.0	41.0
23	38.648692971883584	40.0	35.0	41.0	35.0	41.0
24	38.58377032660884	40.0	35.0	41.0	35.0	41.0
25	38.51130090296576	40.0	35.0	41.0	35.0	41.0
26	38.434848155539306	40.0	35.0	41.0	35.0	41.0
27	38.42650905929541	40.0	35.0	41.0	35.0	41.0
28	38.47447491652012	40.0	36.0	41.0	35.0	41.0
29	38.51581573175792	40.0	36.0	41.0	35.0	41.0
30	38.47641125447037	40.0	35.0	41.0	35.0	41.0
31	38.46052933156824	40.0	35.0	41.0	35.0	41.0
32	38.42231530694908	40.0	35.0	41.0	35.0	41.0
33	38.35245006026358	40.0	35.0	41.0	35.0	41.0
34	38.29389065618146	40.0	35.0	41.0	35.0	41.0
35	38.190937740807335	40.0	35.0	41.0	35.0	41.0
36	38.16763253047756	40.0	35.0	41.0	35.0	41.0
37	38.109854577068226	40.0	35.0	41.0	35.0	41.0
38	38.010982790302506	40.0	35.0	41.0	35.0	41.0
39	37.982146173756696	40.0	35.0	41.0	35.0	41.0
40	37.89387880105115	40.0	35.0	41.0	35.0	41.0
41	37.86149354883326	40.0	35.0	41.0	35.0	41.0
42	37.815194325344294	40.0	35.0	41.0	35.0	41.0
43	37.74574400821956	40.0	35.0	41.0	35.0	41.0
44	37.601499673983916	39.0	35.0	41.0	35.0	41.0
45	37.581130584260336	39.0	35.0	41.0	35.0	41.0
46	37.525901483867145	39.0	35.0	41.0	35.0	41.0
47	37.43198118985991	39.0	35.0	41.0	35.0	41.0
48	37.371789729505444	39.0	35.0	41.0	35.0	41.0
49	37.32149730295785	39.0	35.0	41.0	35.0	41.0
50	37.22723617395428	38.0	35.0	41.0	34.0	41.0
51	37.12573254826026	38.0	35.0	41.0	34.0	41.0
52	36.675482602596276	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	0.0
12	2.0
13	3.0
14	5.0
15	20.0
16	27.0
17	60.0
18	87.0
19	146.0
20	257.0
21	350.0
22	559.0
23	954.0
24	1457.0
25	2260.0
26	3173.0
27	3960.0
28	4343.0
29	4895.0
30	5987.0
31	7483.0
32	10155.0
33	15388.0
34	53609.0
35	174164.0
36	40123.0
37	66161.0
38	118855.0
39	493562.0
40	4173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.53875639683073	21.38181423010808	23.59743929185355	21.48199008120764
2	27.82152101321847	23.70561735591077	26.215150856533164	22.257710774337593
3	28.88808756989587	23.959909901009663	24.898935014127364	22.2530675149671
4	26.727984035091186	25.911461935152435	23.75244512062595	23.608108909130426
5	24.50702416470728	34.347177491059256	21.076939795696585	20.06885854853688
6	85.28165813755902	2.8257691015787083	8.405287388117207	3.4872853727450552
7	87.53887494813381	2.424374147912509	5.784216869850427	4.252534034103258
8	77.20041097785067	4.379779099405267	11.164568967220564	7.255240955523503
9	50.77562190037739	18.24316848116022	14.7745549386497	16.206654679812686
10	40.43123036494043	20.266147675406533	24.183280314556125	15.119341645096915
11	36.16200035565391	18.62974452194187	23.88966825393689	21.318586868467328
12	22.86133449250163	26.266029124103458	29.653830195016894	21.21880618837802
13	18.26608839975499	29.07450949398352	30.72612673134299	21.933275374918495
14	17.850170911461934	23.231510936357708	37.52454999901207	21.39376815316828
15	20.08585090197783	19.514236035644426	38.4218845705479	21.97802849182984
16	21.638082630258243	22.635099089130822	25.797455098693963	29.929363181916973
17	21.963011993440162	23.9443006461046	29.184169449329193	24.90851791112604
18	24.700163995969255	26.83082729050997	26.463417043725673	22.005591669795106
19	26.55193535002272	23.573926616743396	24.221710695303393	25.65242733793049
20	23.222718381379543	28.929679318725178	26.983264507715717	20.864337792179565
21	22.138467921993243	22.376657248424255	27.406393866945923	28.078480962636583
22	20.9357649522831	26.482484044970462	26.028827725198077	26.552923277548356
23	21.460848432159015	22.409654027780523	32.99994072434846	23.129556815712
24	24.470470846258717	21.26049672995989	28.74839461777084	25.520637806010548
25	19.537057161486633	27.452530082393157	27.209104740076267	25.801308016043944
26	19.23188634881745	26.562604967299595	27.52425362075438	26.681255063128567
27	23.28476022998953	24.343324573709275	29.860504633380096	22.511410562921107
28	20.625456916480605	25.120625950880243	27.71640552449072	26.537511608148428
29	22.742486811167534	23.45893185275928	27.294264092786154	26.504317243287034
30	28.637450356641835	24.062950741933573	22.819248779909508	24.480350121515084
31	27.221947797909547	25.091976052636777	21.564975993361127	26.121100156092545
32	25.286202604176957	25.03843038074727	24.841931595898124	24.83343541917765
33	20.269605421746263	26.09531524767343	26.61456995514809	27.020509375432216
34	21.23362510126257	25.0176839027089	27.81559344806465	25.93309754796388
35	26.19825729584478	25.791527533540137	25.24994566398609	22.760269506628994
36	23.059512754144357	27.04777617513979	26.124261524174585	23.768449546541266
37	22.332793266285986	29.219142083736738	25.895358716484555	22.55270593349272
38	19.96423702357195	27.703858844915136	25.427377447590445	26.90452668392247
39	25.329276244294718	23.52591333899745	27.349390448716683	23.79541996799115
40	19.353697812728456	21.924877990950584	30.667147458062477	28.054276738258483
41	20.834996344668156	25.461757325482605	24.760131196775404	28.94311513307384
42	21.25051866195096	20.945249056529214	28.421588192290216	29.382644089229615
43	23.01604394301634	24.45347849281777	27.506075754282666	25.02440180988323
44	21.28924542095592	25.793108217581157	25.90563316275118	27.012013198711742
45	22.095493074628045	25.042876054612634	25.6553911205074	27.20623975025192
46	23.802335460670605	22.46399004169054	27.49678923554168	26.23688526209717
47	20.586927742980777	22.828732884155617	31.28144079350339	25.302898579360217
48	20.75902471794669	21.013613641303273	28.480765051075853	29.74659658967418
49	21.1612100136334	23.516330441998775	29.530240461559742	25.79221908280809
50	19.872063385430046	24.96127324099504	28.521368872379522	26.645294501195394
51	21.454920867005196	20.61636798324475	28.206911540969354	29.721799608780703
52	21.797435340143448	20.12852937108534	31.99739187133232	26.076643417438895
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	103.0
1	203.0
2	303.0
3	1322.5
4	2342.0
5	1614.5
6	887.0
7	907.0
8	927.0
9	973.5
10	1020.0
11	1064.0
12	1108.0
13	1044.0
14	947.0
15	914.0
16	922.0
17	930.0
18	908.0
19	886.0
20	981.5
21	1077.0
22	1194.5
23	1312.0
24	1388.5
25	1465.0
26	1950.5
27	2436.0
28	3419.0
29	4402.0
30	5121.0
31	5840.0
32	6443.5
33	7047.0
34	8523.0
35	9999.0
36	11274.0
37	12549.0
38	14275.5
39	19995.5
40	23989.0
41	34500.0
42	45011.0
43	61134.0
44	77257.0
45	93620.0
46	109983.0
47	113763.5
48	117544.0
49	111763.5
50	105983.0
51	92859.0
52	79735.0
53	72095.0
54	64455.0
55	59601.5
56	54748.0
57	52199.0
58	49650.0
59	50230.0
60	50810.0
61	47363.0
62	43916.0
63	43741.0
64	34816.0
65	26066.0
66	21410.0
67	16754.0
68	13953.5
69	11153.0
70	9489.5
71	7826.0
72	6144.0
73	4462.0
74	3888.0
75	3314.0
76	2554.0
77	1794.0
78	1414.0
79	1034.0
80	738.0
81	442.0
82	281.5
83	121.0
84	83.5
85	46.0
86	29.5
87	13.0
88	14.0
89	7.5
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1012220.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.56534905023884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.4451504506038	18.00954860166276
2	11.754504151898484	6.010160031115327
3	4.176492148219612	3.203204387244487
4	1.9595522037332964	2.003865442824258
5	1.1734831852210854	1.5000253617381565
6	0.8074658077275907	1.2385887132413338
7	0.5939133582982556	1.062852161734613
8	0.45879007550486295	0.9383302736853801
9	0.3873580624558183	0.8912649666696489
>10	7.781354642684748	48.981119443735594
>50	0.35100924100705594	5.8757235597023705
>100	0.09773958568752734	4.567748491549644
>500	0.0065935434789204704	1.20078657215009
>1k	0.005817832481400415	3.143264602572903
>5k	7.757109975200554E-4	1.373517390373497
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	8073	0.7975538914465236	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	5779	0.5709233170654601	No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTT	4808	0.47499555432613466	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	3795	0.37491849597913496	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	3234	0.319495761790915	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	2741	0.27079093477702476	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2713	0.26802473770524193	No Hit
TGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTTG	2533	0.250242042243781	Illumina Single End Adapter 1 (95% over 22bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1763	0.17417162276975362	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1693	0.16725613009029658	No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1346	0.13297504495070242	No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	1297	0.1281342000750825	No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1271	0.12556558850842703	No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1239	0.12240422042638952	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1182	0.11677303353026022	No Hit
GGGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT	1065	0.10521428148031062	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1020	0.10076860761494538	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4855663788504475	0.0
2	9.879275256367193E-5	0.0	0.0	1.679081622572168	0.0
3	9.879275256367193E-5	0.0	0.0	2.5398628756594417	0.0
4	9.879275256367193E-5	0.0	0.0	3.113058426033866	0.0
5	9.879275256367193E-5	0.0	0.0	6.049771788741578	0.0
6	9.879275256367193E-5	0.0	0.0	6.514888067811345	0.0
7	9.879275256367193E-5	0.0	0.0	8.219754598802632	0.0
8	9.879275256367193E-5	0.0	0.0	10.192547074746596	0.0
9	9.879275256367193E-5	0.0	0.0	11.721858884432239	0.0
10	9.879275256367193E-5	0.0	0.0	12.851751595502954	0.0
11	9.879275256367193E-5	0.0	0.0	13.916144711623955	0.0
12	9.879275256367193E-5	0.0	0.0	14.840548497362233	0.0
13	9.879275256367193E-5	0.0	0.0	15.266740431921914	0.0
14	9.879275256367193E-5	0.0	0.0	15.543162553595069	0.0
15	9.879275256367193E-5	0.0	0.0	16.41570014423742	0.0
16	9.879275256367193E-5	0.0	0.0	17.312738337515558	0.0
17	9.879275256367193E-5	0.0	0.0	18.42366284009405	0.0
18	9.879275256367193E-5	0.0	0.0	19.069174685345082	0.0
19	9.879275256367193E-5	0.0	0.0	19.7669478967023	0.0
20	9.879275256367193E-5	0.0	0.0	20.616861947007568	0.0
21	2.963782576910158E-4	0.0	0.0	21.34842227974156	0.0
22	2.963782576910158E-4	0.0	0.0	22.11604196716129	0.0
23	2.963782576910158E-4	0.0	0.0	22.700598684080536	0.0
24	2.963782576910158E-4	0.0	0.0	23.2152101321847	0.0
25	2.963782576910158E-4	0.0	0.0	23.722115745588905	0.0
26	2.963782576910158E-4	0.0	0.0	24.21360968959317	0.0
27	2.963782576910158E-4	0.0	0.0	24.7030289857936	0.0
28	2.963782576910158E-4	0.0	0.0	25.208057536899094	0.0
29	2.963782576910158E-4	0.0	0.0	25.76109936575053	0.0
30	2.963782576910158E-4	0.0	0.0	26.53761040090099	0.0
31	2.963782576910158E-4	0.0	0.0	27.045701527335954	0.0
32	2.963782576910158E-4	0.0	0.0	27.555768508822194	0.0
33	2.963782576910158E-4	0.0	0.0	28.11157653474541	0.0
34	2.963782576910158E-4	0.0	0.0	28.803619766453934	0.0
35	2.963782576910158E-4	0.0	0.0	29.293533026417183	0.0
36	2.963782576910158E-4	0.0	0.0	29.797573649997037	0.0
37	2.963782576910158E-4	0.0	0.0	30.37689435103041	0.0
38	2.963782576910158E-4	0.0	0.0	31.097686273734958	0.0
39	2.963782576910158E-4	0.0	0.0	31.631957479599297	0.0
40	2.963782576910158E-4	0.0	0.0	32.14153049732272	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACG	45	3.092282E-10	46.0	25
CAATCGT	20	6.3110964E-4	46.0	11
CCCGCTA	20	6.3110964E-4	46.0	14
ACGGGTA	95	0.0	43.578945	4
TCGGCGT	1100	0.0	43.072727	4
CTAACGG	75	0.0	42.933334	1
GCGTAGG	150	0.0	42.933334	1
AGTACGG	70	0.0	42.714287	1
GTCGGCG	1115	0.0	42.286995	3
CTTGCGG	370	0.0	42.27027	1
TAGACGG	60	1.8189894E-12	42.166668	1
GTCCCCC	1130	0.0	42.132744	9
CACGAGG	110	0.0	41.81818	1
CGTTTTT	1025	0.0	41.736588	1
GCGTCCC	1145	0.0	41.580784	7
CGATAGT	50	8.712959E-10	41.399998	9
TAGTAGG	200	0.0	41.399998	1
CTAGCGG	290	0.0	41.241383	1
TCCCCCA	1160	0.0	40.84483	10
ATTGCGG	250	0.0	40.48	1
>>END_MODULE