FastQCFastQC Report
Thu 26 May 2016
SRR1544818_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544818_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1354379
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT58570.4324491150556824No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTT51760.38216776840160693Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT47780.35278160692095784No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA28180.2080658368152489No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23400.17277290920783622No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC20720.15298524268317804No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT18990.1402118609340517No Hit
ACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC17570.12972735105904626No Hit
GCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC16650.12293457001326807No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA16390.1210148710220699No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC15630.11540344320164445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTC351.01974365E-746.00000439
GTATTCG351.01974365E-746.0000048
ACTACGG551.8189894E-1246.0000041
GCGATAT351.01974365E-746.0000048
CGGGTAC650.046.05
GCGAATG206.311856E-446.019
ACGTTAG253.4174816E-546.017
GCAACTA206.311856E-446.08
TTACTCG253.4174816E-546.038
TGCGCAA301.8616502E-646.033
GTATGAT405.6134013E-946.029
TACGACA206.311856E-446.09
CGCTACC206.311856E-446.016
GAACGAT206.311856E-446.07
TACTCGA253.4174816E-546.039
ACCACGG301.8616502E-646.01
CGTAAGC301.8616502E-646.023
ACGTAAG301.8616502E-646.022
CTCTCGA253.4174816E-546.010
ATACCGG405.6134013E-946.01
Produced by FastQC (version 0.11.5)