FastQCFastQC Report
Thu 26 May 2016
SRR1544817_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544817_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences823921
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT60170.7302884621219753No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTT46550.5649813513674248Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT35030.4251621211256905No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA30430.3693315257166646No Hit
TGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTTG23750.2882557915139923Illumina Single End Adapter 1 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA13890.16858412396334113No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT13730.16664219020998372No Hit
ACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC12920.15681115058361178No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC12440.1509853493235395No Hit
GCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC12190.14795107783391856No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC11600.14079019711841306No Hit
CTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTTGAAA9800.11894344239314206Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9560.11603054176310593No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT9490.11518094574601205No Hit
AGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC9200.11166119081805173No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGG351.019107E-746.0000041
ACGGGTA700.046.0000044
GTCCGCA351.019107E-746.00000430
GCGATAA351.019107E-746.0000048
ATGGGCG600.046.04
CGTATGA206.3104084E-446.038
CGACGGT405.6079443E-946.027
GGGTCGA301.860757E-646.012
GACCGGA206.3104084E-446.010
TGCGGTA206.3104084E-446.020
TCCTACG206.3104084E-446.036
CTGCGTA206.3104084E-446.035
GTGGACG206.3104084E-446.029
GACCAAC206.3104084E-446.040
TACTCGT206.3104084E-446.035
ACGTCCA206.3104084E-446.016
ATGCGGT206.3104084E-446.019
CGTAAAA301.860757E-646.013
TTACGGC206.3104084E-446.02
CGGTCTA405.6079443E-946.030
Produced by FastQC (version 0.11.5)