##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544817_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	823921
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89525816188688	34.0	33.0	34.0	31.0	34.0
2	33.247336819913556	34.0	33.0	34.0	31.0	34.0
3	33.350286010430615	34.0	34.0	34.0	31.0	34.0
4	36.582832577395166	37.0	37.0	37.0	35.0	37.0
5	36.58359964122774	37.0	37.0	37.0	35.0	37.0
6	36.50988990449327	37.0	37.0	37.0	35.0	37.0
7	36.406545044973974	37.0	37.0	37.0	35.0	37.0
8	36.435576954586665	37.0	37.0	37.0	35.0	37.0
9	38.274546952923885	39.0	39.0	39.0	37.0	39.0
10	38.11900291411434	39.0	39.0	39.0	35.0	39.0
11	37.485769873567975	39.0	35.0	39.0	35.0	39.0
12	37.42622654356425	39.0	35.0	39.0	35.0	39.0
13	37.4089639662055	39.0	35.0	39.0	35.0	39.0
14	38.66536840303864	41.0	35.0	41.0	35.0	41.0
15	38.736850984499725	41.0	35.0	41.0	35.0	41.0
16	38.737632612835455	41.0	35.0	41.0	35.0	41.0
17	38.82083840562384	41.0	36.0	41.0	35.0	41.0
18	38.815002894695	41.0	36.0	41.0	35.0	41.0
19	38.73246585534293	41.0	35.0	41.0	35.0	41.0
20	38.65885078787893	41.0	35.0	41.0	35.0	41.0
21	38.59722109279895	41.0	35.0	41.0	35.0	41.0
22	38.58229733190439	41.0	35.0	41.0	35.0	41.0
23	38.55474614677863	41.0	35.0	41.0	35.0	41.0
24	38.485493148008125	40.0	35.0	41.0	35.0	41.0
25	38.43455622565756	40.0	35.0	41.0	35.0	41.0
26	38.38389966999263	40.0	35.0	41.0	35.0	41.0
27	38.390740131638836	40.0	35.0	41.0	35.0	41.0
28	38.431378736553626	40.0	36.0	41.0	35.0	41.0
29	38.46786160323623	40.0	36.0	41.0	35.0	41.0
30	38.42558085059126	40.0	35.0	41.0	35.0	41.0
31	38.402936689318516	40.0	35.0	41.0	35.0	41.0
32	38.36973933180487	40.0	35.0	41.0	35.0	41.0
33	38.3001525631705	40.0	35.0	41.0	35.0	41.0
34	38.25067573226074	40.0	35.0	41.0	35.0	41.0
35	38.16972379633484	40.0	35.0	41.0	35.0	41.0
36	38.13625335438713	40.0	35.0	41.0	35.0	41.0
37	38.07977949342231	40.0	35.0	41.0	35.0	41.0
38	38.02489073588366	40.0	35.0	41.0	35.0	41.0
39	37.97221092798946	40.0	35.0	41.0	35.0	41.0
40	37.921175695242624	40.0	35.0	41.0	35.0	41.0
41	37.895794621086246	40.0	35.0	41.0	35.0	41.0
42	37.84805460717715	40.0	35.0	41.0	35.0	41.0
43	37.78704147606385	40.0	35.0	41.0	35.0	41.0
44	37.658043671662696	40.0	35.0	41.0	35.0	41.0
45	37.64026890927649	40.0	35.0	41.0	35.0	41.0
46	37.574636403247396	39.0	35.0	41.0	35.0	41.0
47	37.53226340874914	39.0	35.0	41.0	35.0	41.0
48	37.48973627325921	39.0	35.0	41.0	35.0	41.0
49	37.45047158647492	39.0	35.0	41.0	35.0	41.0
50	37.38347972681847	39.0	35.0	41.0	35.0	41.0
51	37.31242801190891	39.0	35.0	41.0	35.0	41.0
52	36.9038754929174	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	2.0
14	8.0
15	23.0
16	14.0
17	24.0
18	61.0
19	105.0
20	169.0
21	305.0
22	453.0
23	721.0
24	1254.0
25	1959.0
26	2815.0
27	3224.0
28	3341.0
29	3704.0
30	4349.0
31	5608.0
32	7382.0
33	11337.0
34	37992.0
35	170609.0
36	28526.0
37	43759.0
38	78445.0
39	411795.0
40	5932.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.735324139086153	22.039248908572546	23.76004495576639	22.465381996574916
2	25.483632532730688	24.407315749932334	27.23343621536531	22.87561550197167
3	26.932193741875736	23.981061291070382	26.193166577863654	22.893578389190225
4	26.102138433175025	26.91459496723594	23.767205836481896	23.216060763107144
5	24.282910618857876	34.76619724463875	20.324035920919602	20.626856215583775
6	85.0267197947376	3.014002556070303	8.05077185798153	3.9085057912105654
7	86.6702026043759	2.4373696021827334	6.109809071500787	4.782618721940574
8	75.98398390136919	5.604056699610763	11.058827241932176	7.353132157087876
9	49.60062918653609	21.60110010547128	14.30537636496703	14.492894343025606
10	40.25689356139727	19.81986137020418	26.120222691253165	13.803022377145382
11	39.31457020758058	18.06398914459032	23.360613456872688	19.260827190956416
12	19.86270528363763	33.76719369939594	26.920663510215174	19.449437506751256
13	13.996608898183199	35.693713353586084	28.54205682340904	21.767620924821678
14	13.01908799508691	25.056042994413296	40.38057046731422	21.544298543185572
15	14.86416780249563	18.52483429843395	44.227662603574856	22.383335295495563
16	15.079601078258717	23.444359349986225	26.89359780852776	34.5824417632273
17	18.6173188934376	24.64495989299945	31.28176123681761	25.455959976745348
18	23.766113498745632	27.861894526295604	25.487030916799064	22.884961058159703
19	27.286596651863466	22.845394157935043	25.059077265902918	24.808931924298566
20	19.920477812800012	31.293898322776094	26.72076570447895	22.064858159944947
21	20.502208342790144	25.521136128342402	25.059441378481672	28.917214150385778
22	18.822435646135975	29.368471006322206	22.967129130098638	28.841964217443177
23	18.54486049026545	22.6952584046286	37.37421427539776	21.38566682970819
24	19.422614546783006	22.95207914351012	31.940198150065356	25.68510815964152
25	16.355572925074128	31.909005839152055	26.657895599214005	25.077525636559816
26	16.413709566815264	29.452459641154917	27.223119692300596	26.910711099729223
27	19.44676734784039	25.506450254332634	35.11222556531512	19.934556832511856
28	16.248766568639468	23.342529198794544	33.2744280094815	27.1342762230845
29	17.598410527222878	26.889835311880628	29.503314031320965	26.008440129575526
30	24.125613984835926	25.917290614027316	26.091821910110312	23.865273491026446
31	24.816699659311997	25.488365996254498	25.139060662369328	24.555873682064178
32	21.839593844555484	25.658163828813684	27.333203061944044	25.16903926468678
33	15.459127756180507	26.126655346811162	29.68354975780445	28.73066713920388
34	17.245949550988506	21.679748422482252	32.965539171838074	28.108762854691165
35	23.816361034613756	22.420596149388107	27.61429797274253	26.148744843255606
36	18.74026757419704	27.900975943081924	30.005789390002196	23.352967092718842
37	18.661619257186064	27.096651256613196	29.464353985394233	24.77737550080651
38	17.079064619059352	27.98423635275712	27.440252160097874	27.496446868085656
39	25.019146253099507	21.804153553556713	27.76127808369977	25.41542210964401
40	15.375745975645724	20.340420986963554	33.476267748971075	30.807565288419642
41	18.712959130790452	23.807865074442816	25.732442794879606	31.746732999887122
42	19.986746302133334	21.53750177504882	30.010280111806836	28.465471811011007
43	20.992789357232063	25.77722864206641	29.04744508272031	24.182536917981214
44	16.990585262422	28.942823401758176	26.78776241896978	27.27882891685004
45	19.75140820539833	26.69090847302108	26.29875922570246	27.258924095878122
46	20.650402162343234	22.08634080209146	29.103275678129336	28.15998135743597
47	19.003520968636558	19.543257181210336	35.34768503290971	26.1055368172434
48	18.130379004783226	19.46388063904185	31.58057629311548	30.825164063059447
49	18.35564332017269	23.67957607586164	32.88919690115921	25.07558370280646
50	17.347779702180187	25.529632088513342	29.21020340542358	27.912384803882894
51	18.592195125503537	20.93101158970338	30.150220712908155	30.326572571884924
52	21.032963111754647	19.493980612218888	33.32977312145218	26.143283154574288
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	120.0
1	176.0
2	232.0
3	1028.5
4	1825.0
5	1323.5
6	822.0
7	869.5
8	917.0
9	958.0
10	999.0
11	1005.0
12	1011.0
13	1006.5
14	976.0
15	950.0
16	908.0
17	866.0
18	889.5
19	913.0
20	930.0
21	947.0
22	1208.5
23	1470.0
24	1716.0
25	1962.0
26	2733.0
27	3504.0
28	5158.0
29	6812.0
30	6844.5
31	6877.0
32	7415.5
33	7954.0
34	10092.5
35	12231.0
36	14389.5
37	16548.0
38	18596.0
39	23909.0
40	27174.0
41	37813.5
42	48453.0
43	64945.5
44	81438.0
45	96786.5
46	112135.0
47	109688.0
48	107241.0
49	92345.0
50	77449.0
51	66925.0
52	56401.0
53	49554.5
54	42708.0
55	38007.0
56	33306.0
57	30886.5
58	28467.0
59	27886.5
60	27306.0
61	27061.5
62	26817.0
63	25926.0
64	20069.0
65	15103.0
66	12214.0
67	9325.0
68	7858.5
69	6392.0
70	5369.0
71	4346.0
72	3493.0
73	2640.0
74	2139.0
75	1638.0
76	1406.5
77	1175.0
78	815.5
79	456.0
80	309.0
81	162.0
82	149.5
83	137.0
84	71.5
85	6.0
86	5.0
87	4.0
88	2.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	823921.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.778609747786927
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.1195457830286	17.374648606497438
2	12.330380745097438	6.110593850487913
3	4.647534887904278	3.4547835982981425
4	2.2042954645096446	2.1847750833560475
5	1.2423965838440723	1.5392430051527952
6	0.7624739699924433	1.1335826967173133
7	0.5444520758581343	0.9443535859842836
8	0.41479846450265934	0.8222503420714113
9	0.34849205718654025	0.7771633816705852
>10	6.56206549931186	44.32329031734996
>50	0.6717259121739201	10.691157223521943
>100	0.1385711098980447	5.952789976226698
>500	0.007862190632513174	1.3710046048744655
>1k	0.004913869145320733	2.5877407519890774
>5k	4.913869145320733E-4	0.7326229758019235
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	6017	0.7302884621219753	No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTT	4655	0.5649813513674248	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	3503	0.4251621211256905	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	3043	0.3693315257166646	No Hit
TGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTTG	2375	0.2882557915139923	Illumina Single End Adapter 1 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1389	0.16858412396334113	No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	1373	0.16664219020998372	No Hit
ACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1292	0.15681115058361178	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1244	0.1509853493235395	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1219	0.14795107783391856	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1160	0.14079019711841306	No Hit
CTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTTGAAA	980	0.11894344239314206	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	956	0.11603054176310593	No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	949	0.11518094574601205	No Hit
AGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	920	0.11166119081805173	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5784535167813419	0.0
2	0.0	0.0	0.0	2.0413364873574045	0.0
3	1.2137085958483884E-4	0.0	0.0	3.0739597607052134	0.0
4	1.2137085958483884E-4	0.0	0.0	3.725721276675798	0.0
5	1.2137085958483884E-4	0.0	0.0	6.848229381214947	0.0
6	1.2137085958483884E-4	0.0	0.0	7.337718057920601	0.0
7	1.2137085958483884E-4	0.0	0.0	9.137405163844592	0.0
8	1.2137085958483884E-4	0.0	0.0	11.208841624379037	0.0
9	1.2137085958483884E-4	0.0	0.0	12.710077786583907	0.0
10	1.2137085958483884E-4	0.0	0.0	13.868319899602024	0.0
11	1.2137085958483884E-4	0.0	0.0	14.926309682603065	0.0
12	1.2137085958483884E-4	0.0	0.0	15.888901969970421	0.0
13	1.2137085958483884E-4	0.0	0.0	16.291731852932504	0.0
14	1.2137085958483884E-4	0.0	0.0	16.5951590018946	0.0
15	1.2137085958483884E-4	0.0	0.0	17.574257726165495	0.0
16	1.2137085958483884E-4	0.0	0.0	18.613799138509638	0.0
17	1.2137085958483884E-4	0.0	0.0	19.869744793493552	0.0
18	1.2137085958483884E-4	0.0	0.0	20.483881342992834	0.0
19	1.2137085958483884E-4	0.0	0.0	21.153120262743638	0.0
20	1.2137085958483884E-4	0.0	0.0	22.017887637285614	0.0
21	1.2137085958483884E-4	0.0	0.0	22.78483009900221	0.0
22	1.2137085958483884E-4	0.0	0.0	23.67799825468704	0.0
23	1.2137085958483884E-4	0.0	0.0	24.314224300630766	0.0
24	1.2137085958483884E-4	0.0	0.0	24.876292751368155	0.0
25	1.2137085958483884E-4	0.0	0.0	25.39891567274047	0.0
26	1.2137085958483884E-4	0.0	0.0	25.859032601426595	0.0
27	1.2137085958483884E-4	0.0	0.0	26.329101940598672	0.0
28	1.2137085958483884E-4	0.0	0.0	26.784121293182235	0.0
29	1.2137085958483884E-4	0.0	0.0	27.39801510096235	0.0
30	1.2137085958483884E-4	0.0	0.0	27.987027882527574	0.0
31	1.2137085958483884E-4	0.0	0.0	28.502004439746045	0.0
32	1.2137085958483884E-4	0.0	0.0	28.976685871582347	0.0
33	1.2137085958483884E-4	0.0	0.0	29.42320926399497	0.0
34	1.2137085958483884E-4	0.0	0.0	30.072300621054687	0.0
35	1.2137085958483884E-4	0.0	0.0	30.532781662319568	0.0
36	1.2137085958483884E-4	0.0	0.0	31.01171107424134	0.0
37	1.2137085958483884E-4	0.0	0.0	31.476440095591688	0.0
38	1.2137085958483884E-4	0.0	0.0	31.90585019680285	0.0
39	1.2137085958483884E-4	0.0	0.0	32.37798284058787	0.0
40	1.2137085958483884E-4	0.0	0.0	32.856184027352136	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACGG	35	1.019107E-7	46.000004	1
ACGGGTA	70	0.0	46.000004	4
GTCCGCA	35	1.019107E-7	46.000004	30
GCGATAA	35	1.019107E-7	46.000004	8
ATGGGCG	60	0.0	46.0	4
CGTATGA	20	6.3104084E-4	46.0	38
CGACGGT	40	5.6079443E-9	46.0	27
GGGTCGA	30	1.860757E-6	46.0	12
GACCGGA	20	6.3104084E-4	46.0	10
TGCGGTA	20	6.3104084E-4	46.0	20
TCCTACG	20	6.3104084E-4	46.0	36
CTGCGTA	20	6.3104084E-4	46.0	35
GTGGACG	20	6.3104084E-4	46.0	29
GACCAAC	20	6.3104084E-4	46.0	40
TACTCGT	20	6.3104084E-4	46.0	35
ACGTCCA	20	6.3104084E-4	46.0	16
ATGCGGT	20	6.3104084E-4	46.0	19
CGTAAAA	30	1.860757E-6	46.0	13
TTACGGC	20	6.3104084E-4	46.0	2
CGGTCTA	40	5.6079443E-9	46.0	30
>>END_MODULE