FastQCFastQC Report
Thu 26 May 2016
SRR1544816_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544816_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences792434
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCTT37290.4705754674837273Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT35490.44786064202192233No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT26560.3351698690364118No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15930.20102620533697443No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC12110.15282029796803268No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA10730.1354055984473155No Hit
TCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT10710.13515321149773987No Hit
ACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC9850.12430057266598858No Hit
CGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC9330.11773851197702269No Hit
GCCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC8780.11079787086369337No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG1550.046.0000041
CCGTAGG351.01903424E-746.0000041
TACGATC551.8189894E-1246.00000423
ACACGAC253.416189E-546.025
CGTATCT206.310262E-446.042
ATTAGCG206.310262E-446.01
TTTCGTA206.310262E-446.039
CGTTGCG253.416189E-546.027
AGGGTAC800.046.05
CTAACGG501.6370905E-1146.01
TCGGACG206.310262E-446.020
TACGCCA206.310262E-446.023
CTTGCGT206.310262E-446.021
ACGTGTA253.416189E-546.035
GTACTTG206.310262E-446.08
GTTCACC253.416189E-546.040
CGTAATT206.310262E-446.044
CGTAAAT206.310262E-446.020
ACGTAAA206.310262E-446.019
CGGTCAT206.310262E-446.05