##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544816_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	792434
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86542601655154	34.0	31.0	34.0	31.0	34.0
2	33.1965804092202	34.0	33.0	34.0	31.0	34.0
3	33.25986391295679	34.0	34.0	34.0	31.0	34.0
4	36.55494463892261	37.0	37.0	37.0	35.0	37.0
5	36.54644298452616	37.0	37.0	37.0	35.0	37.0
6	36.650566482508324	37.0	37.0	37.0	35.0	37.0
7	36.61931593041187	37.0	37.0	37.0	35.0	37.0
8	36.522003094264	37.0	37.0	37.0	35.0	37.0
9	38.329632499362724	39.0	39.0	39.0	37.0	39.0
10	38.11195885083174	39.0	39.0	39.0	35.0	39.0
11	37.30314070320052	39.0	35.0	39.0	35.0	39.0
12	37.17973105646653	39.0	35.0	39.0	35.0	39.0
13	37.16866641259714	39.0	35.0	39.0	35.0	39.0
14	38.405531564773845	40.0	35.0	41.0	35.0	41.0
15	38.48088547437389	40.0	35.0	41.0	35.0	41.0
16	38.46892864263775	40.0	35.0	41.0	35.0	41.0
17	38.5546543939306	40.0	36.0	41.0	35.0	41.0
18	38.52004709540479	40.0	36.0	41.0	35.0	41.0
19	38.43006483820734	40.0	35.0	41.0	35.0	41.0
20	38.33596741179707	40.0	35.0	41.0	35.0	41.0
21	38.288412662758034	40.0	35.0	41.0	35.0	41.0
22	38.267292922817546	40.0	35.0	41.0	35.0	41.0
23	38.219895915622	40.0	35.0	41.0	35.0	41.0
24	38.182379857502326	40.0	35.0	41.0	35.0	41.0
25	38.147484837854	40.0	35.0	41.0	35.0	41.0
26	38.07563405911407	40.0	35.0	41.0	35.0	41.0
27	38.03087323360683	40.0	35.0	41.0	35.0	41.0
28	38.06059558272361	40.0	35.0	41.0	35.0	41.0
29	38.09059808135441	40.0	35.0	41.0	35.0	41.0
30	38.01223571931543	40.0	35.0	41.0	35.0	41.0
31	37.92841548949187	40.0	35.0	41.0	35.0	41.0
32	37.87139622984375	40.0	35.0	41.0	35.0	41.0
33	37.7795425234152	40.0	35.0	41.0	35.0	41.0
34	37.65764846031342	40.0	35.0	41.0	35.0	41.0
35	37.61322078557962	40.0	35.0	41.0	35.0	41.0
36	37.54234422046505	40.0	35.0	41.0	34.0	41.0
37	37.43977416415752	39.0	35.0	41.0	34.0	41.0
38	37.42142563292337	39.0	35.0	41.0	35.0	41.0
39	37.294846006102716	39.0	35.0	41.0	34.0	41.0
40	37.201491859258944	39.0	35.0	41.0	34.0	41.0
41	37.1711877582234	39.0	35.0	41.0	34.0	41.0
42	37.07133969516704	38.0	35.0	41.0	34.0	41.0
43	37.07736795745765	38.0	35.0	41.0	34.0	41.0
44	37.04713578670274	38.0	35.0	41.0	34.0	41.0
45	37.04671808630119	38.0	35.0	41.0	34.0	41.0
46	36.99763387234773	38.0	35.0	41.0	34.0	41.0
47	36.917331916601256	38.0	35.0	41.0	34.0	41.0
48	36.909786808743696	38.0	35.0	41.0	34.0	41.0
49	36.839715357998266	37.0	35.0	41.0	34.0	41.0
50	36.755363853645854	37.0	35.0	41.0	34.0	41.0
51	36.677857083365936	37.0	35.0	41.0	34.0	41.0
52	36.41209236352807	37.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	6.0
15	7.0
16	7.0
17	23.0
18	45.0
19	103.0
20	214.0
21	362.0
22	571.0
23	985.0
24	1548.0
25	2680.0
26	3799.0
27	4484.0
28	4539.0
29	4832.0
30	5892.0
31	7339.0
32	9578.0
33	13705.0
34	45032.0
35	180977.0
36	30217.0
37	44706.0
38	77998.0
39	349807.0
40	2976.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.087550508938286	21.94189042872971	24.39761544810041	20.572943614231594
2	27.064588344265893	23.761221754745506	27.056385768404688	22.117804132583913
3	27.745780721170473	24.069133833227752	25.717220613956492	22.467864831645283
4	25.954590540032356	26.922999265553976	23.43374968767115	23.68866050674252
5	24.294641572673562	35.63262050845875	20.883505755684382	19.189232163183306
6	86.29589341194345	3.099311740788507	7.7707417904834974	2.8340530567845397
7	87.63884437063528	2.547846255965797	5.644003159884608	4.169306213514312
8	77.67864579258335	5.116766822221157	10.748907795475711	6.455679589719774
9	53.0797517521964	19.07704616409695	14.047605226428953	13.795596857277703
10	45.088297574309024	19.78372962290866	21.962459965120125	13.165512837662192
11	41.903679044563965	17.482339223203446	22.959009835519424	17.654971896713164
12	20.907482515894067	35.60006259196349	26.448133219927467	17.044321672214974
13	14.854233917272605	38.15421852166868	28.240585335813456	18.750962225245257
14	13.324264228945248	24.07329821789575	44.88399033862757	17.71844721453143
15	16.132195236448712	17.607649343667738	47.33618194070421	18.923973479179338
16	17.204587385195484	20.38807017366746	26.26553126190951	36.141811179227545
17	20.023118644581125	24.21577065093118	31.58673151328691	24.17437919120078
18	25.535880590686418	26.984329294300853	26.721973060217003	20.75781705479573
19	29.3577761681099	22.724794746313258	24.420204080087426	23.497225005489415
20	20.998972785115228	28.649452194125946	27.869071746038156	22.48250327472067
21	21.333385493302913	23.9392807476711	26.48864132533435	28.238692433691636
22	18.46664832654833	28.785741146896775	24.203025109977613	28.544585416577277
23	20.045202502668992	21.691143994326342	36.656428169412216	21.60722533359245
24	19.659681437192244	21.513211194875538	33.88585043044594	24.941256937486276
25	16.770355638450646	31.06921207318212	27.77202896392633	24.3884033244409
26	17.406370751381186	28.67557424340702	29.937256604335506	23.98079840087629
27	18.57656284308851	25.494489130956016	37.44374925861334	18.485198767342137
28	16.040831160702343	24.749442855808812	33.33463733257281	25.87508865091604
29	18.659219569074523	26.294050987211552	31.04536150644722	24.0013679372667
30	26.090374718904037	24.975329175678983	24.4815341088343	24.45276199658268
31	26.203822652738275	25.217746840746358	23.791634382169367	24.786796124346004
32	23.82924003765613	27.894941408369654	25.321225490072358	22.95459306390185
33	17.834419017861425	24.711206232948108	28.76883122127521	28.685543527915257
34	17.819906768260825	23.817377851026077	29.90924165293261	28.45347372778048
35	25.985886521779733	22.259771791720194	25.77791967532943	25.976422011170648
36	22.054959782139587	26.414060981734757	27.132480433701733	24.398498802423923
37	19.532604608080923	27.269021773422143	25.266078941590088	27.93229467690685
38	19.616018494915664	26.337587736013347	25.433537682633506	28.612856086437482
39	25.845180797391325	22.02644005683754	25.940204483906548	26.188174661864583
40	16.887841763478097	19.914592256263614	31.385957694899513	31.811608285358783
41	21.476867474136647	24.006163289308635	24.876267297970557	29.640701938584158
42	22.341292776433118	22.28236042370721	28.213453738734078	27.162893061125597
43	21.79007967855998	26.323454066837108	27.88901031505463	23.997455939548278
44	17.384539280242898	30.06345007912331	25.348609474101313	27.20340116653248
45	21.15923849809574	25.720123063876617	26.719701577670822	26.40093686035683
46	22.419154150377192	23.124575674441026	27.418687234520476	27.037582940661302
47	22.014704063682274	19.676465169339025	31.737785102607912	26.571045664370786
48	19.848088295050438	18.481539156573294	29.54706638029161	32.12330616808466
49	19.23958335962364	24.790077154690486	30.2394899764523	25.73084950923358
50	18.490120312858863	26.05276906341727	27.271671836392684	28.185438787331186
51	20.98105331169536	21.01209690649316	28.905498754470404	29.101351027341078
52	22.217118397241915	19.25914334821575	32.245587645154046	26.27815060938829
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	202.0
1	311.0
2	420.0
3	1730.5
4	3041.0
5	2081.5
6	1122.0
7	1218.0
8	1314.0
9	1349.5
10	1385.0
11	1350.5
12	1316.0
13	1348.0
14	1287.5
15	1195.0
16	1147.0
17	1099.0
18	1088.0
19	1077.0
20	1288.5
21	1500.0
22	1590.0
23	1680.0
24	1898.0
25	2116.0
26	2496.5
27	2877.0
28	3472.5
29	4068.0
30	4949.5
31	5831.0
32	6731.0
33	7631.0
34	9715.0
35	11799.0
36	12004.0
37	12209.0
38	14323.0
39	19672.5
40	22908.0
41	32609.5
42	42311.0
43	53089.0
44	63867.0
45	69645.5
46	75424.0
47	72979.5
48	70535.0
49	76882.5
50	83230.0
51	85586.5
52	87943.0
53	76897.5
54	65852.0
55	57031.5
56	48211.0
57	41407.5
58	34604.0
59	31930.0
60	29256.0
61	28401.0
62	27546.0
63	24494.0
64	18253.5
65	15065.0
66	12275.5
67	9486.0
68	7472.5
69	5459.0
70	4668.0
71	3877.0
72	3110.5
73	2344.0
74	1813.0
75	1282.0
76	1054.5
77	827.0
78	741.0
79	655.0
80	525.5
81	396.0
82	287.0
83	178.0
84	99.5
85	21.0
86	15.5
87	10.0
88	7.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	792434.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.07598060941471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.35811936374363	16.698647369069626
2	12.568189706615932	6.051829833438615
3	4.948632466019206	3.574295378849955
4	2.3738408014971912	2.2861018042673544
5	1.320864929052276	1.5900559209759266
6	0.8508986004229333	1.2291730922616395
7	0.5822536700741783	0.9812829649326527
8	0.43593819135999134	0.8396511553669177
9	0.32438194299274625	0.7028832032583866
>10	5.900110241597722	39.17359738168661
>50	1.1567496155948658	17.3874744496713
>100	0.17004849158460592	6.507193003983897
>500	0.00629809228091133	1.0973156503601404
>1k	0.0036738871638649423	1.8804987918769704
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCTT	3729	0.4705754674837273	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	3549	0.44786064202192233	No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	2656	0.3351698690364118	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1593	0.20102620533697443	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1211	0.15282029796803268	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1073	0.1354055984473155	No Hit
TCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	1071	0.13515321149773987	No Hit
ACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	985	0.12430057266598858	No Hit
CGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	933	0.11773851197702269	No Hit
GCCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	878	0.11079787086369337	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2619347478780567E-4	0.0	0.0	0.47877804334493473	0.0
2	1.2619347478780567E-4	0.0	0.0	1.5101573127856704	0.0
3	1.2619347478780567E-4	0.0	0.0	2.3282696098350146	0.0
4	1.2619347478780567E-4	0.0	0.0	2.8340530567845397	0.0
5	1.2619347478780567E-4	0.0	0.0	5.439317343778788	0.0
6	1.2619347478780567E-4	0.0	0.0	5.864589353813693	0.0
7	1.2619347478780567E-4	0.0	0.0	7.420302510997761	0.0
8	1.2619347478780567E-4	0.0	0.0	9.363429635780394	0.0
9	1.2619347478780567E-4	0.0	0.0	10.818440400083793	0.0
10	1.2619347478780567E-4	0.0	0.0	11.830385874407206	0.0
11	1.2619347478780567E-4	0.0	0.0	12.803716145445552	0.0
12	1.2619347478780567E-4	0.0	0.0	13.69464207744746	0.0
13	1.2619347478780567E-4	0.0	0.0	14.033219170303141	0.0
14	1.2619347478780567E-4	0.0	0.0	14.286868054626632	0.0
15	1.2619347478780567E-4	0.0	0.0	15.172746247637027	0.0
16	2.5238694957561134E-4	0.0	0.0	16.115537697776723	0.0
17	2.5238694957561134E-4	0.0	0.0	17.491046572963807	0.0
18	2.5238694957561134E-4	0.0	0.0	18.102706345260298	0.0
19	2.5238694957561134E-4	0.0	0.0	18.8156994778114	0.0
20	2.5238694957561134E-4	0.0	0.0	19.73287365256917	0.0
21	2.5238694957561134E-4	0.0	0.0	20.421006670587076	0.0
22	2.5238694957561134E-4	0.0	0.0	21.25514553893447	0.0
23	2.5238694957561134E-4	0.0	0.0	21.839673714151587	0.0
24	2.5238694957561134E-4	0.0	0.0	22.33485690921894	0.0
25	2.5238694957561134E-4	0.0	0.0	22.823478043597323	0.0
26	2.5238694957561134E-4	0.0	0.0	23.270833911720093	0.0
27	2.5238694957561134E-4	0.0	0.0	23.734342544615703	0.0
28	2.5238694957561134E-4	0.0	0.0	24.209460977191792	0.0
29	2.5238694957561134E-4	0.0	0.0	24.815820623547197	0.0
30	2.5238694957561134E-4	0.0	0.0	25.413599113617032	0.0
31	2.5238694957561134E-4	0.0	0.0	25.926070814730313	0.0
32	2.5238694957561134E-4	0.0	0.0	26.4218849771716	0.0
33	2.5238694957561134E-4	0.0	0.0	26.89119850990745	0.0
34	2.5238694957561134E-4	0.0	0.0	27.54223064633774	0.0
35	2.5238694957561134E-4	0.0	0.0	28.034006617585817	0.0
36	2.5238694957561134E-4	0.0	0.0	28.539663871060554	0.0
37	2.5238694957561134E-4	0.0	0.0	29.00355108438053	0.0
38	2.5238694957561134E-4	0.0	0.0	29.46945739329711	0.0
39	2.5238694957561134E-4	0.0	0.0	29.980414772712933	0.0
40	2.5238694957561134E-4	0.0	0.0	30.522668133876135	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGG	155	0.0	46.000004	1
CCGTAGG	35	1.01903424E-7	46.000004	1
TACGATC	55	1.8189894E-12	46.000004	23
ACACGAC	25	3.416189E-5	46.0	25
CGTATCT	20	6.310262E-4	46.0	42
ATTAGCG	20	6.310262E-4	46.0	1
TTTCGTA	20	6.310262E-4	46.0	39
CGTTGCG	25	3.416189E-5	46.0	27
AGGGTAC	80	0.0	46.0	5
CTAACGG	50	1.6370905E-11	46.0	1
TCGGACG	20	6.310262E-4	46.0	20
TACGCCA	20	6.310262E-4	46.0	23
CTTGCGT	20	6.310262E-4	46.0	21
ACGTGTA	25	3.416189E-5	46.0	35
GTACTTG	20	6.310262E-4	46.0	8
GTTCACC	25	3.416189E-5	46.0	40
CGTAATT	20	6.310262E-4	46.0	44
CGTAAAT	20	6.310262E-4	46.0	20
ACGTAAA	20	6.310262E-4	46.0	19
CGGTCAT	20	6.310262E-4	46.0	5
>>END_MODULE