FastQCFastQC Report
Thu 26 May 2016
SRR1544815_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544815_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences597141
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT48860.8182322098130927No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCTT41270.6911265513505185Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT29630.4961977154474404No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA22300.3734461375119109No Hit
TGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCTTG20700.34665179580702044Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT11540.19325418954652251No Hit
CGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC10570.17701011988793267No Hit
ACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC10270.17198618081826572No Hit
GCCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC9490.15892393923713158No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC8370.14016790004370827No Hit
AGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC7570.12677072919126303No Hit
ACTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT7260.1215793254859405No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7160.11990467912938484No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT6180.10349314483513944No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA5980.10014385212202813No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGG206.309006E-446.01
TCGTTCA405.6043064E-946.015
ATCTCGA206.309006E-446.039
AGATCTA206.309006E-446.015
CCGATTT206.309006E-446.011
CGACCCG206.309006E-446.031
TAACGGG600.046.02
CGTTACG206.309006E-446.020
GTTACGA206.309006E-446.021
CTAACGG206.309006E-446.01
TACGATC206.309006E-446.023
ACTACGG206.309006E-446.01
ATAAGCG600.046.032
CTCGAGG1000.046.01
CGGTCTA206.309006E-446.030
ACGTAGG301.8598894E-646.01
GGCGATA551.8189894E-1246.07
TTCGCCC206.309006E-446.014
AGGGCGC206.309006E-446.05
TCGAACC206.309006E-446.012
Produced by FastQC (version 0.11.5)