##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544815_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	597141
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.87280558528053	34.0	33.0	34.0	31.0	34.0
2	33.23114641265631	34.0	33.0	34.0	31.0	34.0
3	33.33164361515957	34.0	34.0	34.0	31.0	34.0
4	36.57408551749084	37.0	37.0	37.0	35.0	37.0
5	36.57173933794531	37.0	37.0	37.0	35.0	37.0
6	36.49067975570259	37.0	37.0	37.0	35.0	37.0
7	36.35745326480681	37.0	37.0	37.0	35.0	37.0
8	36.415169951485495	37.0	37.0	37.0	35.0	37.0
9	38.28250446711915	39.0	39.0	39.0	37.0	39.0
10	38.14736050614511	39.0	39.0	39.0	35.0	39.0
11	37.45826697547145	39.0	35.0	39.0	35.0	39.0
12	37.38441842044006	39.0	35.0	39.0	35.0	39.0
13	37.37500858256258	39.0	35.0	39.0	35.0	39.0
14	38.61195094625892	41.0	35.0	41.0	35.0	41.0
15	38.67642483098631	41.0	35.0	41.0	35.0	41.0
16	38.69012176353659	41.0	35.0	41.0	35.0	41.0
17	38.775275856121084	41.0	36.0	41.0	35.0	41.0
18	38.766207646100334	41.0	36.0	41.0	35.0	41.0
19	38.68651959922364	41.0	35.0	41.0	35.0	41.0
20	38.61023778303617	41.0	35.0	41.0	35.0	41.0
21	38.564312281353985	41.0	35.0	41.0	35.0	41.0
22	38.54806988634175	40.0	35.0	41.0	35.0	41.0
23	38.51846548805056	40.0	35.0	41.0	35.0	41.0
24	38.462237561982846	40.0	35.0	41.0	35.0	41.0
25	38.41840201895365	40.0	35.0	41.0	35.0	41.0
26	38.344593655434814	40.0	35.0	41.0	35.0	41.0
27	38.30905096116328	40.0	35.0	41.0	35.0	41.0
28	38.363989409536444	40.0	35.0	41.0	35.0	41.0
29	38.41674746835337	40.0	36.0	41.0	35.0	41.0
30	38.36458390899302	40.0	35.0	41.0	35.0	41.0
31	38.310037327867285	40.0	35.0	41.0	35.0	41.0
32	38.26326612977505	40.0	35.0	41.0	35.0	41.0
33	38.20531164331372	40.0	35.0	41.0	35.0	41.0
34	38.08284308061245	40.0	35.0	41.0	35.0	41.0
35	38.033563262278086	40.0	35.0	41.0	35.0	41.0
36	37.985765505969276	40.0	35.0	41.0	35.0	41.0
37	37.90091452437532	40.0	35.0	41.0	35.0	41.0
38	37.86809647972589	40.0	35.0	41.0	35.0	41.0
39	37.83294230340908	40.0	35.0	41.0	35.0	41.0
40	37.800119234820585	40.0	35.0	41.0	35.0	41.0
41	37.778367253295286	40.0	35.0	41.0	35.0	41.0
42	37.714437628633775	40.0	35.0	41.0	35.0	41.0
43	37.636780927787576	39.0	35.0	41.0	35.0	41.0
44	37.503373575085284	39.0	35.0	41.0	35.0	41.0
45	37.51090613439707	39.0	35.0	41.0	35.0	41.0
46	37.45971386992352	39.0	35.0	41.0	35.0	41.0
47	37.39142011685682	39.0	35.0	41.0	35.0	41.0
48	37.35525277949429	39.0	35.0	41.0	35.0	41.0
49	37.312942839295914	39.0	35.0	41.0	35.0	41.0
50	37.240949792427585	39.0	35.0	41.0	35.0	41.0
51	37.168320379943765	38.0	35.0	41.0	34.0	41.0
52	36.75620498341263	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	4.0
14	9.0
15	9.0
16	14.0
17	21.0
18	39.0
19	66.0
20	119.0
21	197.0
22	318.0
23	495.0
24	867.0
25	1476.0
26	2213.0
27	2527.0
28	2482.0
29	2829.0
30	3260.0
31	4209.0
32	5579.0
33	8539.0
34	29107.0
35	127008.0
36	21090.0
37	32632.0
38	59583.0
39	290118.0
40	2330.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.0279464983982	21.744445616696893	23.34724964455631	22.880358240348595
2	26.690681095419677	24.261439090600042	26.690178701512707	22.357701112467574
3	27.713052696096902	24.04926139722444	25.782687840895196	22.454998065783457
4	26.19582309705748	26.346038875240517	23.67698751216212	23.78115051553988
5	24.127969775982557	34.38417392207201	21.144419827143	20.343436474802434
6	83.72829867652698	3.06828705448127	9.132851370111917	4.070562898879829
7	85.23045645835741	2.528548533763382	6.745475524206175	5.495519483673035
8	73.69850671784386	5.100637872797212	12.9929112219727	8.207944187386229
9	50.50515707345501	18.41122950860852	15.452631790481645	15.630981627454824
10	42.046853255763715	19.12194942233074	25.16105911334174	13.670138208563806
11	38.87256108691247	17.418834077713637	24.005720591953995	19.702884243419895
12	20.241785440959507	32.899097533078454	28.566117550126354	18.292999475835693
13	14.521695880872358	35.94829361909499	29.586312110540057	19.9436983894926
14	13.080997620327528	24.175362267873084	43.25929721791001	19.484342893889384
15	15.325693596654727	18.00228086833763	46.46390048581491	20.208125049192738
16	16.451558342167093	21.90772363646107	26.604101878785748	35.03661614258609
17	18.60297651643414	24.476296218146132	31.739572395799314	25.18115486962041
18	23.996342572357282	27.521808082178246	27.502047255170893	20.979802090293582
19	27.298577722849377	22.489160851457193	25.077159330878303	25.135102094815124
20	19.74625758405469	29.79346586484599	28.018675656168305	22.441600894931014
21	20.38178587636756	23.9141174362504	26.626709604599245	29.077387082782792
22	17.753428419753458	29.028487409171365	24.346678590148727	28.871405580926446
23	18.74347934574916	21.832029621144756	36.657171421825	22.767319611281085
24	20.078339956559674	21.784302199982918	32.97211211422428	25.165245729233128
25	16.409189789346236	30.17980677930338	28.245255308210286	25.165748123140098
26	17.068498059922195	27.306281096089535	29.771360532939457	25.853860311048816
27	19.583314493561822	24.661344640545533	36.586668810214	19.16867205567864
28	16.063040387446183	24.265793171127086	31.882419730013517	27.788746711413214
29	18.63211536303821	25.624601224836347	30.412582622864615	25.33070078926083
30	24.60591384614354	24.388377284426962	24.521009275866167	26.484699593563327
31	25.32316488065633	23.56026466111019	23.28729730499162	27.829273153241864
32	22.320189034080727	27.282501117826442	25.710678047563306	24.686631800529526
33	17.326393598831764	22.7209319072045	28.843104057500657	31.10957043646308
34	17.899457582045113	22.285188925228717	29.858274678844694	29.957078813881477
35	24.354884357295848	21.521215257368027	25.635988820060923	28.487911565275205
36	22.72344387673933	25.487950082141403	25.858716785482827	25.92988925563644
37	19.023647681200924	26.08244953871866	26.060679136083436	28.83322364399698
38	19.63522853061505	25.17244670856632	24.074213627937123	31.118111132881516
39	26.518862379237063	20.63130818349435	25.861731148924626	26.98809828834396
40	17.275484349592475	18.403861064639678	31.590361405430205	32.73029318033765
41	21.26382211236542	23.259833104744107	24.720124727660636	30.75622005522984
42	21.552363679599964	20.7366434393217	28.979587735559942	28.731405145518394
43	23.074449753073395	24.720962050838914	27.52415258707742	24.680435609010267
44	17.36775736383869	27.726952260856315	26.54900601365507	28.35628436164993
45	20.41377162177777	23.863710580918074	27.02276346792466	28.699754329379495
46	22.84837249493838	20.743007095476614	27.980996113145807	28.4276242964392
47	20.771308618902403	18.352616886129073	33.05735161377296	27.818722881195562
48	19.72800393876823	17.265101542181828	29.004037572365654	34.00285694668428
49	19.088121565928315	22.516792516340363	31.373159772984938	27.021926144746384
50	18.91345595093956	23.356292734881713	28.507839856918217	29.22241145726051
51	20.53585334117068	19.09096846473446	29.26176564663957	31.11141254745529
52	21.559732123568807	17.909003066277478	34.25589601115984	26.27536879899387
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	134.0
1	179.5
2	225.0
3	881.5
4	1538.0
5	1089.0
6	640.0
7	718.0
8	796.0
9	818.0
10	840.0
11	819.5
12	799.0
13	822.5
14	757.5
15	669.0
16	709.0
17	749.0
18	712.0
19	675.0
20	806.5
21	938.0
22	1023.5
23	1109.0
24	1197.5
25	1286.0
26	1588.5
27	1891.0
28	2260.0
29	2629.0
30	3199.0
31	3769.0
32	4308.0
33	4847.0
34	6206.0
35	7565.0
36	7820.0
37	8075.0
38	9609.5
39	13247.0
40	15350.0
41	21681.0
42	28012.0
43	35334.5
44	42657.0
45	47052.0
46	51447.0
47	52165.5
48	52884.0
49	62736.0
50	72588.0
51	75094.0
52	77600.0
53	66760.0
54	55920.0
55	47362.5
56	38805.0
57	32573.5
58	26342.0
59	23537.0
60	20732.0
61	19737.0
62	18742.0
63	17609.0
64	13665.5
65	10855.0
66	8764.0
67	6673.0
68	5264.5
69	3856.0
70	3355.5
71	2855.0
72	2259.5
73	1664.0
74	1337.0
75	1010.0
76	817.0
77	624.0
78	548.0
79	472.0
80	375.5
81	279.0
82	193.5
83	108.0
84	57.0
85	6.0
86	8.5
87	11.0
88	5.5
89	1.5
90	3.0
91	1.5
92	0.0
93	1.5
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	597141.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.304179216726332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.28292895308311	18.91713508794699
2	12.432082513197088	6.788956179549254
3	4.759018207391151	3.89823258090815
4	2.240659029909418	2.447174228648916
5	1.2956001601306635	1.7687649482713488
6	0.827637611162651	1.3558779397012972
7	0.5861212282540486	1.1202491341283412
8	0.4831450913268616	1.0553504129016191
9	0.38663411073382703	0.9501054345698425
>10	7.272274045106805	46.3449486744315
>50	0.32680907147949495	6.061762687622178
>100	0.09601170461544484	4.8567033553717165
>500	0.006154596449708003	1.155485701931972
>1k	0.004923677159766402	3.279253634016943
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	4886	0.8182322098130927	No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCTT	4127	0.6911265513505185	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	2963	0.4961977154474404	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2230	0.3734461375119109	No Hit
TGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCTTG	2070	0.34665179580702044	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	1154	0.19325418954652251	No Hit
CGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	1057	0.17701011988793267	No Hit
ACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	1027	0.17198618081826572	No Hit
GCCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	949	0.15892393923713158	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	837	0.14016790004370827	No Hit
AGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	757	0.12677072919126303	No Hit
ACTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	726	0.1215793254859405	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	716	0.11990467912938484	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT	618	0.10349314483513944	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	598	0.10014385212202813	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7018442880324748	0.0
2	0.0	0.0	0.0	2.3567298175807725	0.0
3	0.0	0.0	0.0	3.5358483172316086	0.0
4	0.0	0.0	0.0	4.3103722571385985	0.0
5	0.0	0.0	0.0	7.988063120770471	0.0
6	0.0	0.0	0.0	8.577873567549373	0.0
7	0.0	0.0	0.0	10.807330262031915	0.0
8	0.0	0.0	0.0	13.368032005841167	0.0
9	0.0	0.0	0.0	15.404736904684153	0.0
10	0.0	0.0	0.0	16.742946808207776	0.0
11	0.0	0.0	0.0	18.023883806337196	0.0
12	0.0	0.0	0.0	19.228122001336367	0.0
13	0.0	0.0	0.0	19.678769335885494	0.0
14	0.0	0.0	0.0	20.017215364545393	0.0
15	0.0	0.0	0.0	21.106237890213535	0.0
16	0.0	0.0	0.0	22.2637534518648	0.0
17	0.0	0.0	0.0	23.855839743042264	0.0
18	0.0	0.0	0.0	24.569574020206282	0.0
19	0.0	0.0	0.0	25.40572494603452	0.0
20	0.0	0.0	0.0	26.428598940618716	0.0
21	0.0	0.0	0.0	27.281328865376853	0.0
22	0.0	0.0	0.0	28.214776744520975	0.0
23	0.0	0.0	0.0	28.885807539592825	0.0
24	0.0	0.0	0.0	29.48432614742582	0.0
25	0.0	0.0	0.0	30.038801556081395	0.0
26	0.0	0.0	0.0	30.530812655637448	0.0
27	0.0	0.0	0.0	31.04660373345659	0.0
28	0.0	0.0	0.0	31.575289588221207	0.0
29	0.0	0.0	0.0	32.22773180873529	0.0
30	0.0	0.0	0.0	32.860915596148985	0.0
31	0.0	0.0	0.0	33.4182379036107	0.0
32	0.0	0.0	0.0	33.933024193615914	0.0
33	0.0	0.0	0.0	34.41917403092402	0.0
34	0.0	0.0	0.0	35.092716795530706	0.0
35	0.0	0.0	0.0	35.59795760130354	0.0
36	0.0	0.0	0.0	36.11810275964973	0.0
37	0.0	0.0	0.0	36.603917667686524	0.0
38	0.0	0.0	0.0	37.077172728049156	0.0
39	0.0	0.0	0.0	37.60217436082935	0.0
40	0.0	0.0	0.0	38.14509470962469	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGG	20	6.309006E-4	46.0	1
TCGTTCA	40	5.6043064E-9	46.0	15
ATCTCGA	20	6.309006E-4	46.0	39
AGATCTA	20	6.309006E-4	46.0	15
CCGATTT	20	6.309006E-4	46.0	11
CGACCCG	20	6.309006E-4	46.0	31
TAACGGG	60	0.0	46.0	2
CGTTACG	20	6.309006E-4	46.0	20
GTTACGA	20	6.309006E-4	46.0	21
CTAACGG	20	6.309006E-4	46.0	1
TACGATC	20	6.309006E-4	46.0	23
ACTACGG	20	6.309006E-4	46.0	1
ATAAGCG	60	0.0	46.0	32
CTCGAGG	100	0.0	46.0	1
CGGTCTA	20	6.309006E-4	46.0	30
ACGTAGG	30	1.8598894E-6	46.0	1
GGCGATA	55	1.8189894E-12	46.0	7
TTCGCCC	20	6.309006E-4	46.0	14
AGGGCGC	20	6.309006E-4	46.0	5
TCGAACC	20	6.309006E-4	46.0	12
>>END_MODULE