FastQCFastQC Report
Thu 26 May 2016
SRR1544814_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544814_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1490359
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT56450.3787678002414184Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCTT52100.34958020181714605Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT46270.31046211013588Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31270.20981521901769976No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC24350.1633834532485126No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC21430.14379085844417352No Hit
TCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT17880.11997109421287085Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC16860.11312710561683462No Hit
CGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC16030.10755797764162862No Hit
GCCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC15280.10252563308571963No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTA206.312061E-446.033
CTATCGG600.046.01
TAGCGAT253.4176475E-546.018
ATTAACG206.312061E-446.010
CGACATA206.312061E-446.034
TAGCACG253.4176475E-546.028
CGGTCTA950.046.030
TCGAATC253.4176475E-546.016
AGTACGG1750.046.01
GTTGCGT206.312061E-446.037
GCGAATA351.0198346E-745.99999624
CGGGTCA950.043.578955
ATACCGG950.043.578951
TAGTAGG2650.043.396231
CCTACGG800.043.1251
CTAACGG800.043.1251
ATTACGG750.042.9333341
TATTAGT750.042.93333430
GTCGAAT1950.042.46153629
ATAGCGG3000.042.1666681
Produced by FastQC (version 0.11.5)