FastQCFastQC Report
Thu 26 May 2016
SRR1544813_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544813_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1116954
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT83140.7443457832641273Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCTT68390.6122902106980234Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA53630.48014510893017975No Hit
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT51690.46277644379267185Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCTTG33960.304041169108128Illumina Single End Adapter 1 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT19160.17153795053332546Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC18150.16249550115761258No Hit
ACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC17720.15864574548280413No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC17100.15309493497494078No Hit
GCCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC16390.14673836165141985No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC15140.1355472114339534No Hit
AGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC14310.1281162876895557No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13710.1227445355851718No Hit
ACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT12890.11540314104251384Illumina Single End Adapter 1 (95% over 21bp)
CTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCTTGAAA11640.1042119908250474Illumina Single End Adapter 1 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGC206.3113763E-446.0000047
ATATGCG206.3113763E-446.00000418
TATGCGT206.3113763E-446.00000419
ATATCGT206.3113763E-446.00000437
ATATCGG206.3113763E-446.00000413
CGGTCTA700.046.00000430
ATGTTCG206.3113763E-446.0000049
TATCGTT206.3113763E-446.00000438
TAGTAGG2100.046.0000041
TACCCGC253.417093E-546.031
CTCGTCG253.417093E-546.046
CAACCTA253.417093E-546.018
TAACCGG1000.046.01
TTACGAG253.417093E-546.019
ATACCGG600.046.01
ATACCCG301.8613555E-646.034
CATGCGG1950.044.820511
AGTACGG1050.043.8095251
TCGGCGT8000.043.74
GTCGAGG900.043.4444471
Produced by FastQC (version 0.11.5)