FastQCFastQC Report
Thu 26 May 2016
SRR1544812_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544812_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1825133
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT80550.44133769977311244TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTT77660.4255032372983229TruSeq Adapter, Index 14 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT60770.3329620361913351TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39630.21713486085671563No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC35700.1956021835121057No Hit
TCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT24510.13429158313394146TruSeq Adapter, Index 15 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC23440.12842899668133775TruSeq Adapter, Index 14 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA23180.12700444296388264No Hit
ACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC22550.1235526397254337TruSeq Adapter, Index 14 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC20270.11106039943390426TruSeq Adapter, Index 15 (95% over 21bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC18270.1001022939150188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGA453.110472E-1046.028
AATGCGC206.3124346E-446.014
ACGGCAT206.3124346E-446.04
CGACTTA206.3124346E-446.021
AGTCGTA206.3124346E-446.04
TGCGTAA206.3124346E-446.012
GTTGCGT206.3124346E-446.020
AAATGCG206.3124346E-446.013
ATCGCAC351.0200165E-745.99999622
AGTACGG1600.044.56251
CTAACGG1550.044.5161321
CGGGTAT2500.044.165
TCACGAC1200.044.08333224
CGGTCTA1150.044.030
CGAATAT3800.042.97368614
TTACGGG5650.042.7433622
ATAGCGG2500.042.321
CTCGAGG2400.042.1666681
TCAAGCG5050.041.90099316
TAACGGG5350.041.7009352
Produced by FastQC (version 0.11.5)