FastQCFastQC Report
Thu 26 May 2016
SRR1544811_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544811_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1268592
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT100560.7926898482727308TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTT86050.6783110724330597TruSeq Adapter, Index 14 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT59700.47060047674902566TruSeq Adapter, Index 15 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA50110.39500485577711353No Hit
TGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTTG41380.32618840415200473TruSeq Adapter, Index 15 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC22960.18098805604954152No Hit
TCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT21810.1719228877369556TruSeq Adapter, Index 15 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC21570.1700310265238942TruSeq Adapter, Index 14 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC20620.1625424092221928TruSeq Adapter, Index 14 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC19750.15568441232484517TruSeq Adapter, Index 15 (95% over 21bp)
CTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTTGAAA16980.13384918082409475TruSeq Adapter, Index 14 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC16960.1336915257230063TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16680.13148435430776798No Hit
ACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT14900.11745305031089585TruSeq Adapter, Index 14 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT13710.10807257179613304No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC13140.1035794014151122No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTGCG253.4173587E-546.011
CGCATGA206.311703E-446.023
CGGTCTA301.8615574E-646.030
ATAGCGG1700.044.647061
TACGGGA2900.043.620693
TCGTTCA850.043.29411715
TTAGCGG2400.043.1251
ACGGGAG4000.042.554
TCAAGCG3300.042.51515216
TCGGCGT7200.042.1666644
GTCGGCG7400.041.6486473
CAGGTCG508.712959E-1041.39999824
GGGCGAT12700.041.2913366
CATACGA3400.041.26470617
ATACGAA3400.041.26470618
TTACGGG3700.041.0270232
ACGACCA3200.040.9687527
CGAATAT1350.040.8888914
CGTATAT451.5887053E-840.88888529
ACTAAGG900.040.8888851
Produced by FastQC (version 0.11.5)