##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544811_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1268592
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85778879261417	34.0	33.0	34.0	31.0	34.0
2	33.22303309495882	34.0	33.0	34.0	31.0	34.0
3	33.32030313922837	34.0	34.0	34.0	31.0	34.0
4	36.55927437663173	37.0	37.0	37.0	35.0	37.0
5	36.562406983490355	37.0	37.0	37.0	35.0	37.0
6	36.48427548021744	37.0	37.0	37.0	35.0	37.0
7	36.34486028604942	37.0	37.0	37.0	35.0	37.0
8	36.38181700657106	37.0	37.0	37.0	35.0	37.0
9	38.242818021869915	39.0	39.0	39.0	37.0	39.0
10	38.151721751358984	39.0	39.0	39.0	35.0	39.0
11	37.70935099701086	39.0	37.0	39.0	35.0	39.0
12	37.68639483774137	39.0	37.0	39.0	35.0	39.0
13	37.6757964735707	39.0	37.0	39.0	35.0	39.0
14	39.01025861742782	41.0	38.0	41.0	35.0	41.0
15	39.061194615762986	41.0	38.0	41.0	35.0	41.0
16	39.05485766897474	41.0	38.0	41.0	35.0	41.0
17	39.11363779686455	41.0	38.0	41.0	35.0	41.0
18	39.10196343662896	41.0	38.0	41.0	35.0	41.0
19	39.05626631730296	41.0	38.0	41.0	35.0	41.0
20	38.99872929988523	41.0	37.0	41.0	35.0	41.0
21	38.93725799941983	41.0	37.0	41.0	35.0	41.0
22	38.92218853658229	41.0	37.0	41.0	35.0	41.0
23	38.89306333320721	41.0	37.0	41.0	35.0	41.0
24	38.814932618209795	41.0	36.0	41.0	35.0	41.0
25	38.76316498921639	41.0	36.0	41.0	35.0	41.0
26	38.71059489575845	41.0	36.0	41.0	35.0	41.0
27	38.688658764993	41.0	36.0	41.0	35.0	41.0
28	38.74712042957862	41.0	36.0	41.0	35.0	41.0
29	38.77298532546319	41.0	36.0	41.0	35.0	41.0
30	38.74413838334153	41.0	36.0	41.0	35.0	41.0
31	38.73368427358836	41.0	36.0	41.0	35.0	41.0
32	38.68630970398678	41.0	36.0	41.0	35.0	41.0
33	38.62963978962503	41.0	36.0	41.0	35.0	41.0
34	38.55501690062684	41.0	36.0	41.0	35.0	41.0
35	38.474114608952284	41.0	36.0	41.0	35.0	41.0
36	38.44608274370326	40.0	36.0	41.0	35.0	41.0
37	38.36503777416222	40.0	35.0	41.0	35.0	41.0
38	38.29906384400974	40.0	35.0	41.0	35.0	41.0
39	38.239393753074275	40.0	35.0	41.0	35.0	41.0
40	38.16506804394163	40.0	35.0	41.0	35.0	41.0
41	38.145435254203086	40.0	35.0	41.0	35.0	41.0
42	38.081392599038935	40.0	35.0	41.0	35.0	41.0
43	38.004247228423324	40.0	35.0	41.0	35.0	41.0
44	37.87377344331353	40.0	35.0	41.0	35.0	41.0
45	37.8531001299078	40.0	35.0	41.0	35.0	41.0
46	37.798430070503365	40.0	35.0	41.0	35.0	41.0
47	37.71104027141902	40.0	35.0	41.0	35.0	41.0
48	37.66367437284801	39.0	35.0	41.0	35.0	41.0
49	37.61597739856471	39.0	35.0	41.0	35.0	41.0
50	37.53304450918814	39.0	35.0	41.0	35.0	41.0
51	37.457907664560395	39.0	35.0	41.0	35.0	41.0
52	37.03561980526442	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	5.0
13	5.0
14	14.0
15	19.0
16	30.0
17	50.0
18	96.0
19	175.0
20	281.0
21	428.0
22	718.0
23	1083.0
24	1822.0
25	2960.0
26	4122.0
27	4981.0
28	5486.0
29	5958.0
30	7181.0
31	8538.0
32	11712.0
33	16896.0
34	51019.0
35	194812.0
36	47696.0
37	76823.0
38	137004.0
39	679937.0
40	8738.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.4578808631932	21.220455434056024	23.527264873182236	21.79439882956853
2	28.54361370716511	23.760988560545865	25.20424218346009	22.491155548828935
3	28.439561340446733	23.56336789133149	25.398236785349425	22.59883398287235
4	26.248786055721617	25.83888279289165	23.54578934756013	24.366541803826607
5	24.68547807332854	34.5602841575542	20.50903679039439	20.24520097872287
6	83.43289252967068	3.257942663992836	9.202407078083418	4.106757728253055
7	84.33160543342541	3.1720206338996304	7.02755495856824	5.468818974106727
8	72.06753629220427	6.34159761373239	13.061646297627604	8.529219796435733
9	46.13934188454601	21.331681107873926	16.281199944505403	16.247777063074654
10	36.447415717582956	21.619716977562526	26.598938035239065	15.333929269615448
11	33.970496424382304	18.74834462143857	25.58600401074577	21.69515494343335
12	21.723059896326006	27.99481630027621	29.54953207965997	20.732591723737816
13	16.12669794543872	30.42018237542094	31.078865387768484	22.37425429137185
14	15.304368938161364	25.625969578871693	37.22654722716208	21.84311425580486
15	17.459435342489943	19.572250179726815	41.285298977133706	21.68301550064954
16	17.50436704630015	23.485801581595975	27.810359832002725	31.199471540101154
17	19.52905268202858	24.08386620757501	30.123002509869206	26.264078600527196
18	22.131386608145093	28.248010392624263	27.439318551591057	22.181284447639587
19	26.485268707354294	22.431088955314237	25.83699093167858	25.24665140565288
20	21.414844173698082	28.52571985319157	27.372472788729553	22.686963184380794
21	20.45196564380037	24.13013797974447	27.11052883827109	28.307367538184064
22	20.18742038417395	27.06662189261796	24.7836183737561	27.96233934945199
23	20.06878492060489	22.619329146013847	34.16654054258579	23.145345390795466
24	21.641552209063278	22.07967573498808	30.21680729501684	26.061964760931804
25	18.861068018716814	28.960217311791343	27.36033334594574	24.818381323546106
26	18.1022740171781	26.848111922509364	27.861991877609192	27.187622182703343
27	21.372119641303115	25.918104481188593	31.90631818583122	20.80345769167707
28	18.075314994891976	26.03185263662391	29.239582150920075	26.65325021756404
29	20.232982708388512	26.623295748357233	26.930486712828078	26.213234830426174
30	23.891211674044925	25.290164213553297	26.68935323571329	24.129270876688487
31	25.22095362417546	28.33282883700985	23.01882717217198	23.427390366642705
32	22.308433286667423	28.919226985508345	24.544534412955464	24.227805314868768
33	17.4620366516579	26.17216567659263	29.332677488112807	27.03312018363666
34	22.176554794606933	25.281808493195605	27.538247127524055	25.003389584673403
35	22.526864429225473	28.46281546785728	25.575283463871756	23.435036639045492
36	21.516610541450678	27.500882868566094	25.972574318614654	25.009932271368573
37	21.564301209529933	27.735552486536253	28.564108870306608	22.136037433627205
38	20.503755344507926	27.949726941364915	24.865204888569377	26.681312825557786
39	25.53389899983604	23.3250722060363	26.634568088085057	24.506460706042603
40	17.781367058912558	21.5456979076015	32.00453731380933	28.66839771967662
41	20.250955389912594	26.31910023080707	24.309470657232584	29.120473722047752
42	20.76010253887775	22.931249763517346	28.43278217109993	27.875865526504978
43	22.330977966123072	26.437262729072863	27.138591446264837	24.09316785853923
44	19.738103346071863	26.68612130614098	26.790804293263715	26.78497105452344
45	20.870066971886942	25.027510815139937	27.29671951265655	26.80570270031657
46	23.245141069784452	23.143690012234035	27.922373781326073	25.68879513665544
47	19.79887938754146	21.611518912305925	33.15352769054196	25.436074009610653
48	19.298403269136177	20.975932372267835	28.85687439302786	30.868789965568126
49	20.05309823804659	23.148498492817236	31.461494318110155	25.33690895102602
50	19.361228826919923	23.693985142583273	30.07003039590349	26.87475563459331
51	20.261202971483343	21.008724633294236	28.892583273424393	29.83748912179803
52	20.618055292797056	20.11986517335755	33.87811053514447	25.383968998700922
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	141.0
1	355.0
2	569.0
3	1997.5
4	3426.0
5	2434.5
6	1443.0
7	1433.5
8	1424.0
9	1446.0
10	1468.0
11	1495.0
12	1522.0
13	1477.5
14	1456.0
15	1479.0
16	1470.5
17	1462.0
18	1636.5
19	1811.0
20	1914.5
21	2018.0
22	2239.0
23	2460.0
24	2868.0
25	3276.0
26	4144.5
27	5013.0
28	6861.5
29	8710.0
30	9914.0
31	11118.0
32	12272.0
33	13426.0
34	16274.0
35	19122.0
36	20406.0
37	21690.0
38	24872.0
39	33199.5
40	38345.0
41	50768.5
42	63192.0
43	86434.0
44	109676.0
45	135196.5
46	160717.0
47	160880.5
48	161044.0
49	142755.5
50	124467.0
51	108255.5
52	92044.0
53	80631.5
54	69219.0
55	62752.5
56	56286.0
57	51973.5
58	47661.0
59	47173.0
60	46685.0
61	47039.0
62	47393.0
63	46173.5
64	36335.0
65	27716.0
66	22496.5
67	17277.0
68	14200.0
69	11123.0
70	9495.0
71	7867.0
72	6306.5
73	4746.0
74	4035.0
75	3324.0
76	2668.5
77	2013.0
78	1565.0
79	1117.0
80	746.5
81	376.0
82	287.0
83	198.0
84	133.0
85	68.0
86	42.0
87	16.0
88	8.0
89	1.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1268592.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.95336421624182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.24451266243904	17.029301802257677
2	13.09353778228025	6.534556343207246
3	5.1148951162348455	3.829015222898539
4	2.538212457787421	2.533477596694873
5	1.4703663880355922	1.8345294005986037
6	0.8699036701855656	1.3024213869111552
7	0.6236640711616412	1.0893761701396412
8	0.4708639047775078	0.9399710809755966
9	0.37811183399863263	0.849164607741514
>10	6.509038638276782	43.00805896437349
>50	0.5342510653795481	8.584615190077503
>100	0.13680813460500102	6.122439052251097
>500	0.008550508412595206	1.4809828885917649
>1k	0.006017024438492923	2.5196702098473094
>5k	9.500564902883563E-4	1.5477579027777764
>10k+	3.166854967627854E-4	0.7946621806562504
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	10056	0.7926898482727308	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTT	8605	0.6783110724330597	TruSeq Adapter, Index 14 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	5970	0.47060047674902566	TruSeq Adapter, Index 15 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	5011	0.39500485577711353	No Hit
TGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTTG	4138	0.32618840415200473	TruSeq Adapter, Index 15 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2296	0.18098805604954152	No Hit
TCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	2181	0.1719228877369556	TruSeq Adapter, Index 15 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	2157	0.1700310265238942	TruSeq Adapter, Index 14 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	2062	0.1625424092221928	TruSeq Adapter, Index 14 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	1975	0.15568441232484517	TruSeq Adapter, Index 15 (95% over 21bp)
CTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTTGAAA	1698	0.13384918082409475	TruSeq Adapter, Index 14 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	1696	0.1336915257230063	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1668	0.13148435430776798	No Hit
ACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	1490	0.11745305031089585	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT	1371	0.10807257179613304	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1314	0.1035794014151122	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6913964458234011	0.0
2	0.0	0.0	0.0	2.273228902594372	0.0
3	0.0	0.0	0.0	3.4101586640937356	0.0
4	0.0	0.0	0.0	4.138682886223466	0.0
5	0.0	0.0	0.0	7.59582277044156	0.0
6	0.0	0.0	0.0	8.152897070137602	0.0
7	0.0	0.0	0.0	10.014724986441662	0.0
8	0.0	0.0	0.0	12.257368799424874	0.0
9	0.0	0.0	0.0	13.85291724999054	0.0
10	0.0	0.0	0.0	15.164607691046452	0.0
11	0.0	0.0	0.0	16.39494809994072	0.0
12	0.0	0.0	0.0	17.474176094441713	0.0
13	7.882755054422541E-5	0.0	0.0	17.948402638515773	0.0
14	7.882755054422541E-5	0.0	0.0	18.294455585404922	0.0
15	7.882755054422541E-5	0.0	0.0	19.340418353576247	0.0
16	7.882755054422541E-5	0.0	0.0	20.487832179297992	0.0
17	7.882755054422541E-5	0.0	0.0	21.758611121621453	0.0
18	7.882755054422541E-5	0.0	0.0	22.51023181606064	0.0
19	7.882755054422541E-5	0.0	0.0	23.293068220515345	0.0
20	7.882755054422541E-5	0.0	0.0	24.276284258453465	0.0
21	7.882755054422541E-5	0.0	0.0	25.166877924502124	0.0
22	7.882755054422541E-5	0.0	0.0	26.105556396382763	0.0
23	7.882755054422541E-5	0.0	0.0	26.832267584849976	0.0
24	7.882755054422541E-5	0.0	0.0	27.46943067589895	0.0
25	7.882755054422541E-5	0.0	0.0	28.05519820399309	0.0
26	7.882755054422541E-5	0.0	0.0	28.586101756908448	0.0
27	7.882755054422541E-5	0.0	0.0	29.128356477102173	0.0
28	1.5765510108845082E-4	0.0	0.0	29.681568226821547	0.0
29	1.5765510108845082E-4	0.0	0.0	30.323303315802086	0.0
30	1.5765510108845082E-4	0.0	0.0	30.958180407885276	0.0
31	1.5765510108845082E-4	0.0	0.0	31.54946586451751	0.0
32	1.5765510108845082E-4	0.0	0.0	32.0909323092058	0.0
33	1.5765510108845082E-4	0.0	0.0	32.613086004010746	0.0
34	1.5765510108845082E-4	0.0	0.0	33.27310908471755	0.0
35	1.5765510108845082E-4	0.0	0.0	33.79542043462358	0.0
36	1.5765510108845082E-4	0.0	0.0	34.368575554630645	0.0
37	1.5765510108845082E-4	0.0	0.0	34.8948282820639	0.0
38	1.5765510108845082E-4	0.0	0.0	35.40476370653449	0.0
39	1.5765510108845082E-4	0.0	0.0	35.94686077162713	0.0
40	1.5765510108845082E-4	0.0	0.0	36.51449796309609	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGTGCG	25	3.4173587E-5	46.0	11
CGCATGA	20	6.311703E-4	46.0	23
CGGTCTA	30	1.8615574E-6	46.0	30
ATAGCGG	170	0.0	44.64706	1
TACGGGA	290	0.0	43.62069	3
TCGTTCA	85	0.0	43.294117	15
TTAGCGG	240	0.0	43.125	1
ACGGGAG	400	0.0	42.55	4
TCAAGCG	330	0.0	42.515152	16
TCGGCGT	720	0.0	42.166664	4
GTCGGCG	740	0.0	41.648647	3
CAGGTCG	50	8.712959E-10	41.399998	24
GGGCGAT	1270	0.0	41.291336	6
CATACGA	340	0.0	41.264706	17
ATACGAA	340	0.0	41.264706	18
TTACGGG	370	0.0	41.027023	2
ACGACCA	320	0.0	40.96875	27
CGAATAT	135	0.0	40.88889	14
CGTATAT	45	1.5887053E-8	40.888885	29
ACTAAGG	90	0.0	40.888885	1
>>END_MODULE