FastQCFastQC Report
Thu 26 May 2016
SRR1544810_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544810_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2338943
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT130160.5564906883151919Illumina PCR Primer Index 2 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCTT119750.5119834044694548Illumina PCR Primer Index 2 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT97230.4157005963805018Illumina PCR Primer Index 2 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45320.19376273812572603No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT39710.16977754481404633Illumina PCR Primer Index 2 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC36540.15622441419051253Illumina PCR Primer Index 2 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC36350.15541208144020613Illumina PCR Primer Index 2 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC32160.1374980065781851Illumina PCR Primer Index 2 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC28260.12082380801926339Illumina PCR Primer Index 2 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT26860.1148381982801633Illumina PCR Primer Index 2 (96% over 25bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC25710.10992144742304535No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA24870.1063300815795853No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT24170.10333727671003524Illumina PCR Primer Index 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCG23670.10119955894607094No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTG206.3128007E-446.038
TCCGCGA301.8622359E-646.032
TCCTCGA206.3128007E-446.042
CGTTATG206.3128007E-446.015
CAAATCG206.3128007E-446.044
CGGCTAG206.3128007E-446.019
ACGTAGG2000.046.01
ATACTCG351.0201802E-745.99999643
ATTGCGG5450.045.1559641
GCGATAT950.043.578958
ATTACGG2100.042.7142871
GGCACCG5750.042.47
GCGATAG1900.042.3684238
ACACGTG3750.042.31999641
ACACGAC4100.042.07317425
AATGCGG3400.041.9411771
ACGGGTA2200.041.818184
CGAATAT3750.041.70666514
CGTTTTT35650.041.6129041
CGTAGGG9450.041.3756562
Produced by FastQC (version 0.11.5)