##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544809_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1436990
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86475340816568	34.0	33.0	34.0	31.0	34.0
2	33.22178024899269	34.0	33.0	34.0	31.0	34.0
3	33.31582265708182	34.0	34.0	34.0	31.0	34.0
4	36.57338325249306	37.0	37.0	37.0	35.0	37.0
5	36.56996638807507	37.0	37.0	37.0	35.0	37.0
6	36.50069450726867	37.0	37.0	37.0	35.0	37.0
7	36.36506656274574	37.0	37.0	37.0	35.0	37.0
8	36.40596594269967	37.0	37.0	37.0	35.0	37.0
9	38.245417156695595	39.0	39.0	39.0	37.0	39.0
10	38.150584903165644	39.0	39.0	39.0	35.0	39.0
11	37.73615752371276	39.0	37.0	39.0	35.0	39.0
12	37.71000633268151	39.0	37.0	39.0	35.0	39.0
13	37.69330197148206	39.0	37.0	39.0	35.0	39.0
14	39.04163007397407	41.0	38.0	41.0	35.0	41.0
15	39.094217078754895	41.0	38.0	41.0	35.0	41.0
16	39.10432014140669	41.0	38.0	41.0	35.0	41.0
17	39.148435966847366	41.0	38.0	41.0	35.0	41.0
18	39.14450065762462	41.0	38.0	41.0	35.0	41.0
19	39.105368861300356	41.0	38.0	41.0	35.0	41.0
20	39.0339327343962	41.0	38.0	41.0	35.0	41.0
21	38.97927542989165	41.0	38.0	41.0	35.0	41.0
22	38.95621959790952	41.0	37.0	41.0	35.0	41.0
23	38.92839616142075	41.0	37.0	41.0	35.0	41.0
24	38.8549057404714	41.0	37.0	41.0	35.0	41.0
25	38.80810931182541	41.0	37.0	41.0	35.0	41.0
26	38.75549795057725	41.0	37.0	41.0	35.0	41.0
27	38.73090696525376	41.0	36.0	41.0	35.0	41.0
28	38.7774319932637	41.0	37.0	41.0	35.0	41.0
29	38.795700039666244	41.0	37.0	41.0	35.0	41.0
30	38.754122854021254	41.0	36.0	41.0	35.0	41.0
31	38.71958259973973	41.0	36.0	41.0	35.0	41.0
32	38.700020876972005	41.0	36.0	41.0	35.0	41.0
33	38.62221449001037	41.0	36.0	41.0	35.0	41.0
34	38.55520219347386	41.0	36.0	41.0	35.0	41.0
35	38.497422389856574	40.0	36.0	41.0	35.0	41.0
36	38.45902198345152	40.0	36.0	41.0	35.0	41.0
37	38.39260328881899	40.0	35.0	41.0	35.0	41.0
38	38.34677763937118	40.0	36.0	41.0	35.0	41.0
39	38.3071726316815	40.0	35.0	41.0	35.0	41.0
40	38.25228637638397	40.0	35.0	41.0	35.0	41.0
41	38.240902163550196	40.0	35.0	41.0	35.0	41.0
42	38.20091093187844	40.0	35.0	41.0	35.0	41.0
43	38.12900855259953	40.0	35.0	41.0	35.0	41.0
44	37.99057752663553	40.0	35.0	41.0	35.0	41.0
45	37.963438854828496	40.0	35.0	41.0	35.0	41.0
46	37.909364017842854	40.0	35.0	41.0	35.0	41.0
47	37.848727548556354	40.0	35.0	41.0	35.0	41.0
48	37.78416064134058	40.0	35.0	41.0	35.0	41.0
49	37.754859811132995	40.0	35.0	41.0	35.0	41.0
50	37.67223084363844	39.0	35.0	41.0	35.0	41.0
51	37.60565974711028	39.0	35.0	41.0	35.0	41.0
52	37.192021517199144	39.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	2.0
14	10.0
15	20.0
16	32.0
17	56.0
18	101.0
19	194.0
20	294.0
21	455.0
22	766.0
23	1262.0
24	2092.0
25	3213.0
26	4626.0
27	5573.0
28	5873.0
29	6538.0
30	7825.0
31	10034.0
32	13098.0
33	19379.0
34	57016.0
35	208099.0
36	52938.0
37	84406.0
38	154229.0
39	793355.0
40	5502.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.41692704890083	21.81239952957223	23.222499808627756	22.548173612899188
2	26.83456391484979	24.255005254037954	26.099972859936393	22.81045797117586
3	27.557881404881034	23.448319055804145	26.67276738181894	22.321032157495875
4	26.29183223265298	26.54374769483434	23.565021329306397	23.599398743206283
5	24.164747145074077	33.90462007390448	21.154774911446843	20.775857869574597
6	83.51143710116284	3.166340753937049	9.176194684722928	4.146027460177176
7	84.77602488535064	2.8840840924432323	6.896220572168213	5.443670450037927
8	73.35597324963987	5.70866881467512	12.849358729009944	8.085999206675064
9	44.37490866324748	23.660707450991307	15.930730206890791	16.033653678870415
10	34.0744890361102	22.309201873360287	28.687743129736464	14.928565960793048
11	33.96022240934175	18.900270704736986	26.11771828613978	21.021788599781488
12	22.1267371380455	28.032693338158232	29.546552168073543	20.294017355722726
13	15.479022122631333	30.500351429028733	30.94245610616636	23.07817034217357
14	14.64505668097899	26.45766498027126	37.09629155387303	21.800986784876724
15	16.80060404038998	20.056089464784026	41.31608431513094	21.827222179695056
16	16.954049784619237	24.232736483900375	28.321908990320043	30.49130474116034
17	18.48989902504541	24.79613636838113	29.90285249027481	26.811112116298652
18	21.79305353551521	28.07041106757876	27.90005497602628	22.236480420879758
19	25.43810325750353	22.859936394825294	26.50025400315938	25.20170634451179
20	21.186299139172853	29.078838405277697	27.57611396043118	22.158748495118267
21	20.220947953708794	24.8697624896485	27.148971113229734	27.760318443412967
22	19.234998155867473	27.977508542161043	24.89272715885288	27.894766143118606
23	19.320802510803833	22.743025351603002	35.07533107398103	22.860841063612135
24	21.599941544478387	22.536900048017035	29.792622078093796	26.070536329410782
25	18.25217990382675	29.413774626128227	26.5424950765141	25.791550393530922
26	17.583072951099172	26.45001009053647	27.752176424331417	28.214740534032945
27	21.78101448165958	24.600101601263752	30.448994077898945	23.169889839177728
28	17.694834341227146	24.264956610693186	29.56290579614333	28.477303251936338
29	21.049624562453463	24.628772642815886	27.234845058072775	27.086757736657873
30	22.63919721083654	24.40796386892045	25.190502369536322	27.762336550706685
31	22.935302263759663	26.550219556155575	26.500880312319502	24.01359786776526
32	24.464331693331197	26.366571792427223	23.149291226800464	26.019805287441113
33	16.834494324943112	24.57372702663206	29.655529961934324	28.93624868649051
34	19.948155519523446	24.615550560546698	28.782107043194454	26.654186876735398
35	22.976986617860945	25.788697207357046	26.025233300162142	25.209082874619863
36	20.64294114781592	26.634701702865016	26.90617192882344	25.81618522049562
37	20.51378228101796	27.237698244246655	29.619412800367435	22.62910667436795
38	19.218296578264287	27.52204260294087	26.273669267009513	26.985991551785325
39	24.974843248735205	23.533914641020466	27.35356543886875	24.137676671375583
40	16.95474568368604	21.961461109680652	31.90885114023062	29.174942066402686
41	19.478075699900486	26.49552188950515	24.193000647186132	29.833401763408236
42	21.2686935886819	22.22952143021176	27.88704166347713	28.61474331762921
43	23.038921634806087	24.612140655119383	27.56365736713547	24.78528034293906
44	19.61287134913952	25.575056193849644	27.121900639531244	27.69017181747959
45	19.91412605515696	24.795231699594293	27.18007780151567	28.110564443733082
46	21.92026388492613	23.306494825990438	28.08203258199431	26.691208707089125
47	19.48816623636908	20.596663859873765	33.305938106736996	26.60923179702016
48	19.33242402521938	20.47140202784988	29.081413231824854	31.11476071510588
49	19.46304428005762	22.701758536941803	31.945385841237588	25.889811341762993
50	18.630679406258917	23.095080689496793	30.909331310586712	27.364908593657578
51	19.406954815273593	21.354776303244975	29.04007682725697	30.198192054224453
52	19.868266306654885	20.123104544916806	34.326404498291566	25.682224650136742
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	185.0
1	353.5
2	522.0
3	2206.5
4	3891.0
5	2663.0
6	1435.0
7	1499.0
8	1563.0
9	1650.5
10	1738.0
11	1703.5
12	1669.0
13	1576.5
14	1497.5
15	1511.0
16	1527.0
17	1543.0
18	1822.0
19	2101.0
20	2345.0
21	2589.0
22	2965.0
23	3341.0
24	3465.5
25	3590.0
26	5494.0
27	7398.0
28	9651.5
29	11905.0
30	12922.0
31	13939.0
32	15451.0
33	16963.0
34	20935.0
35	24907.0
36	27688.5
37	30470.0
38	32958.0
39	39062.5
40	42679.0
41	53448.0
42	64217.0
43	80763.0
44	97309.0
45	113981.5
46	130654.0
47	152895.0
48	175136.0
49	177518.5
50	179901.0
51	158070.0
52	136239.0
53	115769.0
54	95299.0
55	83378.0
56	71457.0
57	63194.5
58	54932.0
59	52225.0
60	49518.0
61	50293.0
62	51068.0
63	48673.5
64	36604.5
65	26930.0
66	20982.5
67	15035.0
68	12870.5
69	10706.0
70	9248.5
71	7791.0
72	6550.5
73	5310.0
74	4595.5
75	3881.0
76	3164.0
77	2447.0
78	1736.5
79	1026.0
80	795.5
81	565.0
82	444.5
83	324.0
84	176.5
85	29.0
86	35.5
87	42.0
88	25.0
89	11.0
90	14.0
91	8.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1436990.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.66082132728087
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.75122992850888	16.70800975970482
2	13.270654715459518	6.54530489667969
3	5.490026534826494	4.061656903721612
4	2.6455716733973476	2.609678813846698
5	1.5050428620760625	1.8557796555778605
6	0.9834583870462232	1.455173493945765
7	0.6313885084848789	1.0899391437090775
8	0.47035462710827997	0.9279465135661689
9	0.37804323804334933	0.8390571072636374
>10	5.985075939346767	39.73383559190856
>50	0.7260522724594166	11.264620896455863
>100	0.14554526912632443	6.149445929723511
>500	0.01047699406952915	1.738000745390453
>1k	0.0059464020394624905	2.586366330819703
>5k	5.663240037583324E-4	0.8380307342774248
>10k+	5.663240037583324E-4	1.597153483409154
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	12217	0.8501798899087676	Illumina PCR Primer Index 2 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCTT	10655	0.7414804556747089	Illumina PCR Primer Index 2 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	6882	0.47891773777131363	Illumina PCR Primer Index 2 (96% over 25bp)
TGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCTTG	5119	0.356230732294588	Illumina PCR Primer Index 2 (96% over 27bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	3602	0.2506628438611264	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	2835	0.19728738543761615	Illumina PCR Primer Index 2 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	2791	0.19422542954369898	Illumina PCR Primer Index 2 (96% over 25bp)
ACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	2484	0.1728613281929589	Illumina PCR Primer Index 2 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	2258	0.15713400928329355	Illumina PCR Primer Index 2 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1995	0.1388318638264706	Illumina PCR Primer Index 2 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	1899	0.13215123278519683	Illumina PCR Primer Index 2 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1879	0.13075943465159812	No Hit
CTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCTTGAAA	1694	0.11788530191581012	Illumina PCR Primer Index 2 (96% over 27bp)
CGTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	1668	0.11607596434213181	Illumina PCR Primer Index 2 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1651	0.11489293592857291	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCG	1598	0.11120467087453637	No Hit
GGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1466	0.10201880319278492	Illumina PCR Primer Index 2 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7575557241177739	0.0
2	0.0	0.0	0.0	2.4458764500796804	0.0
3	0.0	0.0	0.0	3.6821411422487285	0.0
4	0.0	0.0	0.0	4.432459516071789	0.0
5	0.0	0.0	0.0	8.019332076075687	0.0
6	0.0	0.0	0.0	8.577651897368806	0.0
7	0.0	0.0	0.0	10.480100766184872	0.0
8	0.0	0.0	0.0	12.748592544137399	0.0
9	0.0	0.0	0.0	14.319863047063654	0.0
10	0.0	0.0	0.0	15.696420991099451	0.0
11	0.0	0.0	0.0	16.978754201490617	0.0
12	0.0	0.0	0.0	18.097203181650535	0.0
13	0.0	0.0	0.0	18.59414470525195	0.0
14	6.958990667993514E-5	0.0	0.0	18.96081392354853	0.0
15	6.958990667993514E-5	0.0	0.0	20.049687193369472	0.0
16	6.958990667993514E-5	0.0	0.0	21.23737813067593	0.0
17	6.958990667993514E-5	0.0	0.0	22.518249953026814	0.0
18	6.958990667993514E-5	0.0	0.0	23.273578800130828	0.0
19	6.958990667993514E-5	0.0	0.0	24.089729225673107	0.0
20	6.958990667993514E-5	0.0	0.0	25.11945107481611	0.0
21	6.958990667993514E-5	0.0	0.0	26.071440998197623	0.0
22	6.958990667993514E-5	0.0	0.0	27.09002846227183	0.0
23	6.958990667993514E-5	0.0	0.0	27.815712009130195	0.0
24	6.958990667993514E-5	0.0	0.0	28.482244135310616	0.0
25	1.3917981335987028E-4	0.0	0.0	29.072018594423064	0.0
26	1.3917981335987028E-4	0.0	0.0	29.651076207906804	0.0
27	1.3917981335987028E-4	0.0	0.0	30.219347385855155	0.0
28	1.3917981335987028E-4	0.0	0.0	30.78323439968267	0.0
29	1.3917981335987028E-4	0.0	0.0	31.442598765475054	0.0
30	1.3917981335987028E-4	0.0	0.0	32.09904035518689	0.0
31	1.3917981335987028E-4	0.0	0.0	32.706281880876	0.0
32	2.0876972003980543E-4	0.0	0.0	33.2382967174441	0.0
33	2.7835962671974055E-4	0.0	0.0	33.772260071399245	0.0
34	2.7835962671974055E-4	0.0	0.0	34.41617547790868	0.0
35	2.7835962671974055E-4	0.0	0.0	34.950556371303904	0.0
36	2.7835962671974055E-4	0.0	0.0	35.551604395298504	0.0
37	3.4794953339967573E-4	0.0	0.0	36.089603963841085	0.0
38	3.4794953339967573E-4	0.0	0.0	36.61528611890132	0.0
39	3.4794953339967573E-4	0.0	0.0	37.16330663400581	0.0
40	3.4794953339967573E-4	0.0	0.0	37.718773269125045	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTAG	20	6.311985E-4	46.0	9
CGTAGAT	20	6.311985E-4	46.0	11
GCGATAT	60	0.0	46.0	8
ACGCTAG	20	6.311985E-4	46.0	9
GCTACTT	30	1.861732E-6	46.0	16
CGACTAC	25	3.4175853E-5	46.0	21
CGGTAAG	20	6.311985E-4	46.0	13
ATTGCGG	320	0.0	45.28125	1
ATTACGG	135	0.0	44.2963	1
ACGGGTA	165	0.0	43.21212	4
CGGGTAT	245	0.0	43.183674	5
TACGGGA	405	0.0	42.592594	3
GTCCCCC	510	0.0	42.39216	9
AGTACGG	125	0.0	42.32	1
GGCGATA	245	0.0	42.2449	7
TACGCGG	180	0.0	42.166664	1
ATCGCGG	355	0.0	42.112675	1
CAACCGG	55	4.7293724E-11	41.81818	1
ACCGGGT	50	8.731149E-10	41.4	3
CTAACGG	95	0.0	41.157894	1
>>END_MODULE