FastQCFastQC Report
Thu 26 May 2016
SRR1544808_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544808_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1714564
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT80270.4681656677732648Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTT72910.4252393028198422Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT60180.3509930221327404Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32670.19054406834623847No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC26360.1537417092625297No Hit
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT23520.1371777314815895Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC23450.13676946442360857No Hit
ACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC23120.13484477686455565No Hit
GCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC21000.12248011739427633No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA17420.10160017357182351No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAC206.3123263E-446.00000420
AATCCCG206.3123263E-446.00000412
GCACGGT206.3123263E-446.00000425
GACCATA206.3123263E-446.00000440
CTCGGTA206.3123263E-446.00000412
TATTACG206.3123263E-446.00000424
TCCCGTA206.3123263E-446.00000414
ACCCGTA206.3123263E-446.00000425
ACGTAGC206.3123263E-446.00000436
AGGCGTA253.417864E-546.05
TACGGGT301.861943E-646.03
CTAACGG1500.046.01
GTTCACG301.861943E-646.042
GAACCGT253.417864E-546.041
CAATACG253.417864E-546.036
AATACGC253.417864E-546.037
CATGCGG2600.045.1153871
CGGTCTA1850.044.75675630
ACGTAGG1650.044.606061
ATAGCGG1600.043.1250041
Produced by FastQC (version 0.11.5)