##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544808_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1714564
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86161963041333	34.0	31.0	34.0	31.0	34.0
2	33.194282628120035	34.0	33.0	34.0	31.0	34.0
3	33.25884714714645	34.0	34.0	34.0	31.0	34.0
4	36.54962952680682	37.0	37.0	37.0	35.0	37.0
5	36.543233731724214	37.0	37.0	37.0	35.0	37.0
6	36.64893932218337	37.0	37.0	37.0	35.0	37.0
7	36.61837994965484	37.0	37.0	37.0	35.0	37.0
8	36.50457259104939	37.0	37.0	37.0	35.0	37.0
9	38.28917147449731	39.0	39.0	39.0	37.0	39.0
10	38.10329273214648	39.0	39.0	39.0	35.0	39.0
11	37.517621972699764	39.0	35.0	39.0	35.0	39.0
12	37.42590536136301	39.0	35.0	39.0	35.0	39.0
13	37.418004227313766	39.0	35.0	39.0	35.0	39.0
14	38.743205852916546	41.0	36.0	41.0	35.0	41.0
15	38.80502798379063	41.0	36.0	41.0	35.0	41.0
16	38.78437317008872	41.0	36.0	41.0	35.0	41.0
17	38.83631932083025	41.0	36.0	41.0	35.0	41.0
18	38.82705224185274	41.0	36.0	41.0	35.0	41.0
19	38.7620048012206	41.0	36.0	41.0	35.0	41.0
20	38.68233673400351	41.0	35.0	41.0	35.0	41.0
21	38.61844177295219	41.0	35.0	41.0	35.0	41.0
22	38.5852374131266	41.0	35.0	41.0	35.0	41.0
23	38.54757477702786	40.0	35.0	41.0	35.0	41.0
24	38.48564474700274	40.0	35.0	41.0	35.0	41.0
25	38.440298524872794	40.0	35.0	41.0	35.0	41.0
26	38.38183993131781	40.0	35.0	41.0	35.0	41.0
27	38.35092011730096	40.0	35.0	41.0	35.0	41.0
28	38.39176198730406	40.0	36.0	41.0	35.0	41.0
29	38.38628187690865	40.0	36.0	41.0	35.0	41.0
30	38.33161608432231	40.0	35.0	41.0	35.0	41.0
31	38.28142139925952	40.0	35.0	41.0	35.0	41.0
32	38.206530639859466	40.0	35.0	41.0	35.0	41.0
33	38.110899913913975	40.0	35.0	41.0	35.0	41.0
34	38.022918362919086	40.0	35.0	41.0	35.0	41.0
35	37.96734796718	40.0	35.0	41.0	35.0	41.0
36	37.88958592388502	40.0	35.0	41.0	35.0	41.0
37	37.81303526727495	40.0	35.0	41.0	35.0	41.0
38	37.756037686548886	40.0	35.0	41.0	35.0	41.0
39	37.65731754545179	40.0	35.0	41.0	34.0	41.0
40	37.5630778436967	40.0	35.0	41.0	34.0	41.0
41	37.51924279291995	40.0	35.0	41.0	34.0	41.0
42	37.443918103961124	39.0	35.0	41.0	34.0	41.0
43	37.44366439514652	39.0	35.0	41.0	34.0	41.0
44	37.41005060178564	39.0	35.0	41.0	34.0	41.0
45	37.38860491646856	39.0	35.0	41.0	34.0	41.0
46	37.326798533038136	39.0	35.0	41.0	34.0	41.0
47	37.23267256282064	39.0	35.0	41.0	34.0	41.0
48	37.196661075352104	39.0	35.0	41.0	34.0	41.0
49	37.14168266684708	39.0	35.0	41.0	34.0	41.0
50	37.05727986823472	38.0	35.0	41.0	34.0	41.0
51	36.97857472803582	38.0	35.0	41.0	34.0	41.0
52	36.715962192137475	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	4.0
12	3.0
13	6.0
14	9.0
15	16.0
16	25.0
17	65.0
18	122.0
19	247.0
20	421.0
21	736.0
22	1277.0
23	1950.0
24	3290.0
25	5360.0
26	7656.0
27	8976.0
28	9595.0
29	10558.0
30	12430.0
31	15577.0
32	20420.0
33	28927.0
34	84971.0
35	303655.0
36	69266.0
37	104110.0
38	184798.0
39	831978.0
40	8115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.772492598701476	21.460732874363394	24.49596515498984	20.270809371945287
2	26.71110556386347	23.98312340630038	25.872699998366933	23.433071031469225
3	27.594070562545348	25.12061375370065	24.766471242834914	22.518844440919093
4	26.08050793087922	26.59393291822294	22.939301186774014	24.38625796412382
5	24.919046474788924	35.28832986111921	20.524809805874845	19.267813858217018
6	86.35600654160474	3.041647905823288	7.529086111687869	3.0732594408840965
7	87.05286008571275	2.8858648612708535	5.738485119248975	4.322789933767419
8	76.55316453629027	5.656540088325661	10.946048091526475	6.8442472838575865
9	48.70660996031644	22.688450241577453	14.555420503404948	14.04951929470116
10	38.952818325825106	22.24256429039686	23.914242921232454	14.89037446254558
11	36.96741562286389	18.999932344316107	25.330054754444863	18.702597278375144
12	21.26505630585968	31.2074673211382	27.844396592953075	19.68307978004904
13	15.586994711191885	33.444129236353966	30.559722471718757	20.409153580735396
14	15.407823796603685	25.168439323349844	39.7728518737125	19.650885006333972
15	17.295067434053205	19.1445755305722	42.31927183820493	21.24108519716966
16	17.294775814726076	22.21293576676053	27.9940556316358	32.498232786877594
17	20.679018106060784	24.017009572112794	30.130983736973366	25.172988584853055
18	24.176875287245036	27.14007759407056	26.445965271637572	22.23708184704683
19	28.34440709124885	22.733067998628222	25.095534491567534	23.82699041855539
20	22.36819389652413	28.236974531134447	26.98021187893832	22.414619693403104
21	20.812054843097137	24.357737593930583	26.777128179525523	28.053079383446754
22	20.390314972202848	27.858919235444112	24.06675982932104	27.684005963032
23	20.823019729797196	22.429492279086695	35.01403272202146	21.73345526909465
24	20.613287109725857	22.546898220188925	31.285329681481706	25.554484988603516
25	18.276483117573914	30.058370524518185	27.292069587370314	24.373076770537583
26	18.02510725758852	28.0028625353151	27.874608355243662	26.097421851852715
27	20.374334233076162	25.616424933685767	32.85045061018428	21.158790223053792
28	17.493018633308527	24.954390737236988	30.529335737831893	27.023254891622596
29	19.407382868181063	25.79903695633409	28.362429165665443	26.431151009819402
30	24.173375855319488	25.15671622639925	24.067809658898707	26.60209825938256
31	25.341427908202903	25.5327885106651	25.471140184909984	23.654643396222014
32	23.85475257849809	27.169589469976042	24.182124435133364	24.793533516392504
33	17.96474205687277	24.142522530509215	30.009786744618456	27.882948667999564
34	19.01375510042203	24.24884693718053	29.00912418550722	27.728273776890216
35	25.739488289734304	23.94223837663686	26.206370832468195	24.111902501160646
36	20.50556293028432	26.986744151866016	29.218856805578564	23.28883611227111
37	21.26342323762776	26.111127960227794	28.391416126782087	24.234032675362364
38	20.74393256827975	26.043763895660938	27.559251214886118	25.6530523211732
39	25.19806784698617	22.403946426030174	28.757106763002138	23.640878963981514
40	17.31075655385276	21.415648526389216	32.148231270457096	29.12536364930093
41	20.80149822345506	24.4949736492776	25.09559281543296	29.607935311834378
42	22.250496336094773	22.242505966531432	28.443266043145666	27.06373165422813
43	22.081123830898118	25.776174001087153	28.58382655882195	23.558875609192775
44	18.825952253750806	27.883940173711803	26.569436894744086	26.720670677793308
45	21.486861966074176	25.021579830207564	26.88980988752826	26.601748316190005
46	22.304095968421127	22.971320988892803	28.366278540783547	26.358304501902523
47	21.323380171285528	20.413177927449777	32.87751288374187	25.385929017522823
48	19.373555026234072	20.52125205008387	30.746650460408592	29.358542463273462
49	19.443776960206794	24.217876964639405	31.57904866776627	24.759297407387535
50	19.192051157028843	24.637925443436348	29.257583852221323	26.91243954731349
51	20.056469166505302	21.64503628910907	29.813818556787613	28.484675987598013
52	21.915134109896158	19.58462909521021	32.78017035234614	25.720066442547495
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	231.0
1	633.0
2	1035.0
3	3557.5
4	6080.0
5	4137.0
6	2194.0
7	2376.0
8	2558.0
9	2498.0
10	2438.0
11	2398.0
12	2358.0
13	2248.5
14	2307.5
15	2476.0
16	2528.5
17	2581.0
18	2682.0
19	2783.0
20	3182.0
21	3581.0
22	3752.0
23	3923.0
24	4430.5
25	4938.0
26	6570.5
27	8203.0
28	10604.0
29	13005.0
30	14781.0
31	16557.0
32	19075.5
33	21594.0
34	26326.0
35	31058.0
36	32245.5
37	33433.0
38	37580.0
39	47589.0
40	53451.0
41	65613.5
42	77776.0
43	105565.0
44	133354.0
45	145213.0
46	157072.0
47	168740.0
48	180408.0
49	185757.0
50	191106.0
51	172756.5
52	154407.0
53	134337.0
54	114267.0
55	100198.5
56	86130.0
57	77059.0
58	67988.0
59	69962.0
60	71936.0
61	69390.0
62	66844.0
63	60812.0
64	44812.5
65	34845.0
66	28824.0
67	22803.0
68	19001.0
69	15199.0
70	12501.5
71	9804.0
72	7986.5
73	6169.0
74	5493.5
75	4818.0
76	4158.0
77	3498.0
78	2582.5
79	1667.0
80	1233.5
81	800.0
82	556.0
83	312.0
84	242.0
85	172.0
86	112.5
87	53.0
88	28.0
89	3.0
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1714564.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.883549268089276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.48845558441194	14.093984872243215
2	12.983017953413617	5.422629901572018
3	5.669922088954765	3.552242918727435
4	2.927301507149076	2.445297809883989
5	1.6949144438722645	1.7697914646901285
6	1.066052615256356	1.3357777387848917
7	0.7214574781130624	1.0546614952303903
8	0.5193666189664017	0.867697470030864
9	0.37986086393255986	0.7139558760259113
>10	3.9416183251592942	21.11183469578652
>50	2.28755309299092	33.073852774626154
>100	0.3034356221584622	9.83898128510369
>500	0.010338046032635278	1.425724518499253
>1k	0.005867539640144347	2.0486110387161447
>5k	8.382199485920496E-4	1.2449561400794003
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	8027	0.4681656677732648	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTT	7291	0.4252393028198422	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	6018	0.3509930221327404	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3267	0.19054406834623847	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2636	0.1537417092625297	No Hit
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	2352	0.1371777314815895	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	2345	0.13676946442360857	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	2312	0.13484477686455565	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	2100	0.12248011739427633	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1742	0.10160017357182351	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.43602921792362376	0.0
2	0.0	0.0	0.0	1.5089550462974843	0.0
3	0.0	0.0	0.0	2.3753560671984246	0.0
4	0.0	0.0	0.0	2.9267498909343717	0.0
5	0.0	0.0	0.0	5.4799354238162	0.0
6	0.0	0.0	0.0	5.900275521940272	0.0
7	0.0	0.0	0.0	7.375869317214172	0.0
8	0.0	0.0	0.0	9.164137355036033	0.0
9	0.0	0.0	0.0	10.38508915386069	0.0
10	0.0	0.0	0.0	11.39006767901344	0.0
11	0.0	0.0	0.0	12.356844072312262	0.0
12	0.0	0.0	0.0	13.23141043437282	0.0
13	0.0	0.0	0.0	13.602233570750348	0.0
14	0.0	0.0	0.0	13.875597528001288	0.0
15	0.0	0.0	0.0	14.756754486854968	0.0
16	0.0	0.0	0.0	15.726680368886784	0.0
17	0.0	0.0	0.0	16.935092536644884	0.0
18	0.0	0.0	0.0	17.54743479975084	0.0
19	0.0	0.0	0.0	18.227199451289074	0.0
20	0.0	0.0	0.0	19.045949874137097	0.0
21	0.0	0.0	0.0	19.80520995425076	0.0
22	0.0	0.0	0.0	20.649506230155303	0.0
23	5.832386542584587E-5	0.0	0.0	21.266456078629904	0.0
24	5.832386542584587E-5	0.0	0.0	21.800002799545542	0.0
25	5.832386542584587E-5	0.0	0.0	22.305495741191347	0.0
26	5.832386542584587E-5	0.0	0.0	22.768295613345433	0.0
27	5.832386542584587E-5	0.0	0.0	23.24742616781876	0.0
28	5.832386542584587E-5	0.0	0.0	23.711567488877638	0.0
29	5.832386542584587E-5	0.0	0.0	24.31160341637874	0.0
30	1.1664773085169174E-4	0.0	0.0	24.878044797394555	0.0
31	1.1664773085169174E-4	0.0	0.0	25.416549046871392	0.0
32	1.1664773085169174E-4	0.0	0.0	25.896379487729824	0.0
33	1.749715962775376E-4	0.0	0.0	26.361104047442964	0.0
34	1.749715962775376E-4	0.0	0.0	26.976537475416492	0.0
35	1.749715962775376E-4	0.0	0.0	27.464766552896247	0.0
36	1.749715962775376E-4	0.0	0.0	27.992422563403874	0.0
37	1.749715962775376E-4	0.0	0.0	28.459538401599474	0.0
38	1.749715962775376E-4	0.0	0.0	28.91446455192107	0.0
39	1.749715962775376E-4	0.0	0.0	29.416749680968458	0.0
40	1.749715962775376E-4	0.0	0.0	29.950238078018668	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAC	20	6.3123263E-4	46.000004	20
AATCCCG	20	6.3123263E-4	46.000004	12
GCACGGT	20	6.3123263E-4	46.000004	25
GACCATA	20	6.3123263E-4	46.000004	40
CTCGGTA	20	6.3123263E-4	46.000004	12
TATTACG	20	6.3123263E-4	46.000004	24
TCCCGTA	20	6.3123263E-4	46.000004	14
ACCCGTA	20	6.3123263E-4	46.000004	25
ACGTAGC	20	6.3123263E-4	46.000004	36
AGGCGTA	25	3.417864E-5	46.0	5
TACGGGT	30	1.861943E-6	46.0	3
CTAACGG	150	0.0	46.0	1
GTTCACG	30	1.861943E-6	46.0	42
GAACCGT	25	3.417864E-5	46.0	41
CAATACG	25	3.417864E-5	46.0	36
AATACGC	25	3.417864E-5	46.0	37
CATGCGG	260	0.0	45.115387	1
CGGTCTA	185	0.0	44.756756	30
ACGTAGG	165	0.0	44.60606	1
ATAGCGG	160	0.0	43.125004	1
>>END_MODULE