FastQCFastQC Report
Thu 26 May 2016
SRR1544807_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544807_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1136107
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT84510.743855992437332Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTT75350.6632297838143767Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT50180.4416837498580679Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA41260.36317001831693674No Hit
TGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTTG37670.3315708819679837Illumina Single End Adapter 1 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC19450.17119866350616622No Hit
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT18500.16283677505727895Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC18220.16037221846181743No Hit
GCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC17810.1567634034470345No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC16580.14593695840268567No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14050.12366792916512265No Hit
CTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTTGAAA13990.12313980989466661Illumina Single End Adapter 1 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC13970.12296377013784794No Hit
ACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT13670.12032317378556773Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA206.311425E-446.07
TGCGCAA206.311425E-446.018
ACGTCTA206.311425E-446.015
ATGCGTA253.4171302E-546.037
ACGTAGG1100.046.01
CATGCGG1800.044.722221
CGGGTAT1200.044.0833325
TCGTTCA950.043.57894515
TCGATGG950.043.5789451
ATAGCGG1600.043.1251
AAATCGG700.042.7142831
ACGGGAT2700.042.5925944
GCGATGT650.042.461548
CGTTTTT14550.042.364261
GCTACGA1850.042.2702710
CTAACGG1200.042.1666681
GTCGGCG6200.041.9193533
TAACGGG3400.041.2647062
GGACTAA9450.041.132287
TATGCGG2750.040.981821