##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544807_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1136107
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86504968282037	34.0	33.0	34.0	31.0	34.0
2	33.22665030670527	34.0	33.0	34.0	31.0	34.0
3	33.326809006546036	34.0	34.0	34.0	31.0	34.0
4	36.566089285604264	37.0	37.0	37.0	35.0	37.0
5	36.56993487409196	37.0	37.0	37.0	35.0	37.0
6	36.497217251544086	37.0	37.0	37.0	35.0	37.0
7	36.36636425970441	37.0	37.0	37.0	35.0	37.0
8	36.399898072980804	37.0	37.0	37.0	35.0	37.0
9	38.2459328214684	39.0	39.0	39.0	37.0	39.0
10	38.131401355682165	39.0	39.0	39.0	35.0	39.0
11	37.634967481055924	39.0	37.0	39.0	35.0	39.0
12	37.58978775766719	39.0	37.0	39.0	35.0	39.0
13	37.58320827175609	39.0	37.0	39.0	35.0	39.0
14	38.88696663254429	41.0	37.0	41.0	35.0	41.0
15	38.94702347578177	41.0	37.0	41.0	35.0	41.0
16	38.94354404998825	41.0	37.0	41.0	35.0	41.0
17	39.00148577554755	41.0	37.0	41.0	35.0	41.0
18	39.006298702498974	41.0	37.0	41.0	35.0	41.0
19	38.94822670751962	41.0	37.0	41.0	35.0	41.0
20	38.883404468065066	41.0	36.0	41.0	35.0	41.0
21	38.82188033345451	41.0	36.0	41.0	35.0	41.0
22	38.795005223979786	41.0	35.0	41.0	35.0	41.0
23	38.76842410089895	41.0	35.0	41.0	35.0	41.0
24	38.693954882770726	41.0	35.0	41.0	35.0	41.0
25	38.644036169128434	41.0	35.0	41.0	35.0	41.0
26	38.578077593043616	40.0	35.0	41.0	35.0	41.0
27	38.55818950151702	40.0	36.0	41.0	35.0	41.0
28	38.61653875911336	40.0	36.0	41.0	35.0	41.0
29	38.64822327474437	41.0	36.0	41.0	35.0	41.0
30	38.622993256797116	41.0	36.0	41.0	35.0	41.0
31	38.59468782429824	41.0	35.0	41.0	35.0	41.0
32	38.54390035445605	41.0	35.0	41.0	35.0	41.0
33	38.47319134553347	40.0	35.0	41.0	35.0	41.0
34	38.40495745559177	40.0	35.0	41.0	35.0	41.0
35	38.342823343223834	40.0	35.0	41.0	35.0	41.0
36	38.27811464941242	40.0	35.0	41.0	35.0	41.0
37	38.24313554973255	40.0	35.0	41.0	35.0	41.0
38	38.168189263863354	40.0	35.0	41.0	35.0	41.0
39	38.12048953135576	40.0	35.0	41.0	35.0	41.0
40	38.06654478847503	40.0	35.0	41.0	35.0	41.0
41	38.0328358156406	40.0	35.0	41.0	35.0	41.0
42	37.97271735848824	40.0	35.0	41.0	35.0	41.0
43	37.90075934749104	40.0	35.0	41.0	35.0	41.0
44	37.77286382356591	40.0	35.0	41.0	35.0	41.0
45	37.76104979548581	40.0	35.0	41.0	35.0	41.0
46	37.692646027178775	40.0	35.0	41.0	35.0	41.0
47	37.61678345437534	39.0	35.0	41.0	35.0	41.0
48	37.554344793228104	39.0	35.0	41.0	35.0	41.0
49	37.51563805169759	39.0	35.0	41.0	35.0	41.0
50	37.43462895660356	39.0	35.0	41.0	35.0	41.0
51	37.35543395120354	39.0	35.0	41.0	34.0	41.0
52	36.94212516954829	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	6.0
14	11.0
15	12.0
16	21.0
17	45.0
18	85.0
19	161.0
20	276.0
21	382.0
22	638.0
23	1012.0
24	1773.0
25	2697.0
26	3897.0
27	4711.0
28	4823.0
29	5317.0
30	6282.0
31	7836.0
32	10624.0
33	16005.0
34	49707.0
35	190759.0
36	42808.0
37	66629.0
38	122515.0
39	591440.0
40	5633.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.831854746075855	21.665652971066987	23.327644315192142	22.174847967665016
2	26.75223372446433	24.228615790590148	25.95908660011777	23.06006388482775
3	28.23536867566171	24.707707988772185	24.695825305186926	22.361098030379182
4	26.171566586597915	25.92678330474154	23.330196891666013	24.571453216994527
5	24.65392784306408	34.34342011799945	20.66618725172893	20.336464787207543
6	84.15518960802108	3.0891456526541954	8.750408192186123	4.005256547138606
7	85.16372137483529	2.864782982588788	6.717941179836054	5.253554462739865
8	73.28878353887443	5.86634885622569	12.63560562517439	8.209261979725502
9	46.98272257806703	21.88103761353464	15.519929020770052	15.61631078762828
10	37.06420257951056	21.765731572818407	25.967448488566657	15.202617359104382
11	34.91519724814653	18.859931326890866	25.953541347777982	20.271330077184633
12	20.641717725531134	29.707061042665877	29.23184171913385	20.419379512669142
13	15.45470629086873	32.132712851870465	31.259203578536177	21.153377278724626
14	15.083526463616542	25.254135393937364	38.58580221757282	21.076535924873273
15	16.843219872776068	19.22327738496462	41.827662359267215	22.105840382992096
16	16.84277977338402	23.323507380906904	27.919025232658544	31.91468761305053
17	19.559865400001936	24.344978069847294	30.38006103298369	25.715095497167077
18	23.151340498738236	27.43641223934013	26.857241439406675	22.555005822514957
19	27.080107771539126	22.64003302505838	25.37393044845248	24.90592875495002
20	21.531070576979104	28.90660826841134	26.984694223343396	22.577626931266156
21	20.400191179175906	24.208723298069636	26.88664007879539	28.504445443959064
22	19.936678499472322	27.827924658504877	23.89493243154034	28.340464410482465
23	20.0005809311975	22.47684417048746	34.9489088615773	22.573666036737734
24	20.889317643496607	22.76264471568259	30.404794618816716	25.943243022004093
25	18.19256460879125	29.44141704962649	27.05132527129927	25.314693070282996
26	17.71109587389216	26.924928725903456	27.47698940328684	27.886985996917545
27	21.231362890995303	24.955836026008114	31.816633468502527	21.99616761449406
28	17.58205873214407	24.482377100044275	30.110984264686337	27.824579903125326
29	19.390779213577595	25.277548681594254	27.475845144867517	27.855826959960638
30	23.571811457899653	24.831639977572532	23.563449569450764	28.03309899507705
31	24.744764357582515	24.405271686557693	25.783486942691138	25.066477013168655
32	23.088582325432373	26.811119023120185	24.083559030971553	26.01673962047589
33	17.46015120054713	22.959281124049056	30.837060241684984	28.74350743371883
34	18.904997504636448	23.106274320992654	29.173748599383686	28.814979574987216
35	25.527701176033595	23.160406546214396	26.75214570458592	24.559746573166084
36	20.387340276928143	26.081082151593115	29.509896515028956	24.02168105644979
37	21.293504925152295	25.28564651040791	29.189768217254187	24.231080347185607
38	20.705532137377904	25.14067777066773	27.425673814174196	26.728116277780174
39	25.773892775944518	21.385749757725282	29.07463821629477	23.765719250035428
40	17.413852744503817	20.41541861814072	32.48162364988509	29.689104987470373
41	20.796984791045208	24.0575931668408	25.208629116799735	29.936792925314254
42	21.861585220406177	21.216311491787305	29.188535938956456	27.733567348850062
43	22.865804013178337	24.587648874621845	28.575741545470628	23.97080556672919
44	18.913271373206925	26.293738177830082	27.203511641069017	27.589478807893975
45	21.194570581820198	23.611068323670217	27.31424064810797	27.880120446401612
46	22.629822719162895	21.464615568780054	28.850803665499818	27.054758046557236
47	20.624377809484493	19.70201750363302	33.810371734352486	25.86323295253
48	19.236216307090793	19.237008485996476	30.73856599774493	30.7882092091678
49	19.5859192840111	22.52912797826261	32.19828766128543	25.68666507644086
50	19.361556613945694	23.050645757837952	30.05755619849187	27.53024142972449
51	20.01950520505551	20.307946346602918	30.086338698731723	29.586209749609854
52	21.46998478136302	18.893202840929597	34.04459263079974	25.59221974690764
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	119.0
1	313.5
2	508.0
3	1704.0
4	2900.0
5	2052.5
6	1205.0
7	1290.5
8	1376.0
9	1354.5
10	1333.0
11	1339.5
12	1346.0
13	1330.5
14	1398.0
15	1481.0
16	1518.5
17	1556.0
18	1614.0
19	1672.0
20	1856.0
21	2040.0
22	2164.5
23	2289.0
24	2637.0
25	2985.0
26	4029.5
27	5074.0
28	6324.5
29	7575.0
30	8532.0
31	9489.0
32	11137.5
33	12786.0
34	15483.0
35	18180.0
36	19111.0
37	20042.0
38	22654.5
39	28459.5
40	31652.0
41	39522.5
42	47393.0
43	64554.5
44	81716.0
45	91990.5
46	102265.0
47	117217.5
48	132170.0
49	136477.5
50	140785.0
51	125455.0
52	110125.0
53	94482.0
54	78839.0
55	68139.0
56	57439.0
57	50852.5
58	44266.0
59	44638.5
60	45011.0
61	43474.0
62	41937.0
63	39662.0
64	29837.5
65	22288.0
66	18381.5
67	14475.0
68	12171.5
69	9868.0
70	8168.5
71	6469.0
72	5178.0
73	3887.0
74	3599.0
75	3311.0
76	2851.5
77	2392.0
78	1729.0
79	1066.0
80	788.5
81	511.0
82	342.0
83	173.0
84	138.5
85	104.0
86	67.5
87	31.0
88	17.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1136107.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.554969477621803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.14508261100266	16.978553930408086
2	12.711847127934849	6.242780364612694
3	4.862191803378598	3.581729139789131
4	2.4232519810362194	2.380115137237238
5	1.3817388111237248	1.6964277166594253
6	0.8683392957028407	1.2793226941321723
7	0.6072762168828176	1.0438154279030256
8	0.43936585437127085	0.8630892114876625
9	0.3539089303696198	0.7821200684775416
>10	6.366460768851229	42.94458459778002
>50	0.685653667248373	10.587572208288082
>100	0.1372744316969096	5.615860786374963
>500	0.011858785984285967	1.9714589458255594
>1k	0.004671642963506593	2.1791759970507574
>5k	0.001078071453116906	1.853393773973684
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	8451	0.743855992437332	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTT	7535	0.6632297838143767	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	5018	0.4416837498580679	Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	4126	0.36317001831693674	No Hit
TGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTTG	3767	0.3315708819679837	Illumina Single End Adapter 1 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	1945	0.17119866350616622	No Hit
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	1850	0.16283677505727895	Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	1822	0.16037221846181743	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	1781	0.1567634034470345	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1658	0.14593695840268567	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1405	0.12366792916512265	No Hit
CTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTTGAAA	1399	0.12313980989466661	Illumina Single End Adapter 1 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	1397	0.12296377013784794	No Hit
ACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	1367	0.12032317378556773	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6792494016848765	0.0
2	0.0	0.0	0.0	2.1762914936709308	0.0
3	0.0	0.0	0.0	3.281205027343375	0.0
4	0.0	0.0	0.0	4.001559712245413	0.0
5	0.0	0.0	0.0	7.338833402135538	0.0
6	0.0	0.0	0.0	7.889837840978006	0.0
7	0.0	0.0	0.0	9.761052436082165	0.0
8	0.0	0.0	0.0	11.962693654734986	0.0
9	0.0	0.0	0.0	13.570464753759989	0.0
10	0.0	0.0	0.0	14.846841010573828	0.0
11	0.0	0.0	0.0	16.027891739070352	0.0
12	0.0	0.0	0.0	17.067758582598294	0.0
13	0.0	0.0	0.0	17.52194115519049	0.0
14	0.0	0.0	0.0	17.851663619711875	0.0
15	0.0	0.0	0.0	18.856762611268127	0.0
16	0.0	0.0	0.0	19.946800785489394	0.0
17	0.0	0.0	0.0	21.237876361997593	0.0
18	0.0	0.0	0.0	21.924695473225672	0.0
19	8.801987840933996E-5	0.0	0.0	22.67612117520621	0.0
20	8.801987840933996E-5	0.0	0.0	23.598481481057682	0.0
21	8.801987840933996E-5	0.0	0.0	24.44144785658393	0.0
22	8.801987840933996E-5	0.0	0.0	25.334761602560324	0.0
23	8.801987840933996E-5	0.0	0.0	26.004240797741762	0.0
24	8.801987840933996E-5	0.0	0.0	26.574081490563827	0.0
25	8.801987840933996E-5	0.0	0.0	27.096215409288032	0.0
26	8.801987840933996E-5	0.0	0.0	27.58727831093374	0.0
27	8.801987840933996E-5	0.0	0.0	28.094800929841995	0.0
28	8.801987840933996E-5	0.0	0.0	28.590352845286578	0.0
29	8.801987840933996E-5	0.0	0.0	29.21555804162812	0.0
30	8.801987840933996E-5	0.0	0.0	29.810924499188896	0.0
31	8.801987840933996E-5	0.0	0.0	30.384726086539384	0.0
32	8.801987840933996E-5	0.0	0.0	30.886175333837393	0.0
33	8.801987840933996E-5	0.0	0.0	31.350128112933024	0.0
34	8.801987840933996E-5	0.0	0.0	31.96424280459499	0.0
35	8.801987840933996E-5	0.0	0.0	32.451344811712275	0.0
36	8.801987840933996E-5	0.0	0.0	32.97937606228991	0.0
37	8.801987840933996E-5	0.0	0.0	33.43047793913777	0.0
38	8.801987840933996E-5	0.0	0.0	33.89583903628795	0.0
39	8.801987840933996E-5	0.0	0.0	34.40072105884393	0.0
40	8.801987840933996E-5	0.0	0.0	34.92100656012154	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGA	20	6.311425E-4	46.0	7
TGCGCAA	20	6.311425E-4	46.0	18
ACGTCTA	20	6.311425E-4	46.0	15
ATGCGTA	25	3.4171302E-5	46.0	37
ACGTAGG	110	0.0	46.0	1
CATGCGG	180	0.0	44.72222	1
CGGGTAT	120	0.0	44.083332	5
TCGTTCA	95	0.0	43.578945	15
TCGATGG	95	0.0	43.578945	1
ATAGCGG	160	0.0	43.125	1
AAATCGG	70	0.0	42.714283	1
ACGGGAT	270	0.0	42.592594	4
GCGATGT	65	0.0	42.46154	8
CGTTTTT	1455	0.0	42.36426	1
GCTACGA	185	0.0	42.27027	10
CTAACGG	120	0.0	42.166668	1
GTCGGCG	620	0.0	41.919353	3
TAACGGG	340	0.0	41.264706	2
GGACTAA	945	0.0	41.13228	7
TATGCGG	275	0.0	40.98182	1
>>END_MODULE