FastQCFastQC Report
Thu 26 May 2016
SRR1544806_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544806_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences815620
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT41030.5030528922782668Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCTT31610.3875579313896177Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT20910.25636938770505874Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13960.17115813736789193No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA12620.15472891787842377No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC11060.13560236384590865No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC9310.11414629361712561No Hit
ACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC9040.11083592849611339No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT8530.10458301660086805No Hit
TCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT8530.10458301660086805Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAC253.4162775E-546.08
AACGTCA206.3103705E-446.043
CTCACGA253.4162775E-546.023
ACCGGGT253.4162775E-546.03
TACCAGG950.046.01
CCGATAG405.6079443E-946.011
ACGGGTC253.4162775E-546.04
ACGGGTA453.092282E-1046.04
AACGGCG206.3103705E-446.037
CTAACGG551.8189894E-1246.01
GCCGAGT206.3103705E-446.013
TTCCACG206.3103705E-446.042
ACAATCC301.8607334E-646.041
GTCGTTT206.3103705E-446.08
CAAATCG253.4162775E-546.043
CTACCGG405.6079443E-946.01
TCACTAG206.3103705E-446.043
TCCAACG206.3103705E-446.012
ACGTCGA206.3103705E-446.028
CGTAATG206.3103705E-446.043
Produced by FastQC (version 0.11.5)