##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544806_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	815620
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.905524631568625	34.0	33.0	34.0	31.0	34.0
2	33.235917461562984	34.0	33.0	34.0	31.0	34.0
3	33.29291214045757	34.0	34.0	34.0	31.0	34.0
4	36.58403177950516	37.0	37.0	37.0	35.0	37.0
5	36.575310806503026	37.0	37.0	37.0	35.0	37.0
6	36.67693288541233	37.0	37.0	37.0	35.0	37.0
7	36.64933302273118	37.0	37.0	37.0	35.0	37.0
8	36.55435864740933	37.0	37.0	37.0	35.0	37.0
9	38.346564576641086	39.0	39.0	39.0	37.0	39.0
10	38.10446899291337	39.0	39.0	39.0	35.0	39.0
11	37.279978421323655	39.0	35.0	39.0	35.0	39.0
12	37.17778745003801	39.0	35.0	39.0	35.0	39.0
13	37.14740933277752	39.0	35.0	39.0	35.0	39.0
14	38.381663029351905	40.0	35.0	41.0	35.0	41.0
15	38.43835854932444	40.0	35.0	41.0	35.0	41.0
16	38.41902356489542	40.0	35.0	41.0	35.0	41.0
17	38.508174149726585	40.0	36.0	41.0	35.0	41.0
18	38.481121110320984	40.0	36.0	41.0	35.0	41.0
19	38.40181334444962	40.0	35.0	41.0	35.0	41.0
20	38.30760648341139	40.0	35.0	41.0	35.0	41.0
21	38.238974032024714	40.0	35.0	41.0	35.0	41.0
22	38.22331723106348	40.0	35.0	41.0	35.0	41.0
23	38.17951374414556	40.0	35.0	41.0	35.0	41.0
24	38.12036487580001	40.0	35.0	41.0	35.0	41.0
25	38.080949461759154	40.0	35.0	41.0	35.0	41.0
26	38.00003678183468	40.0	35.0	41.0	35.0	41.0
27	37.9771339594419	40.0	35.0	41.0	35.0	41.0
28	38.01452024226968	40.0	35.0	41.0	35.0	41.0
29	38.0213776023148	40.0	35.0	41.0	35.0	41.0
30	37.88200510041441	40.0	35.0	41.0	35.0	41.0
31	37.85009318064785	40.0	35.0	41.0	35.0	41.0
32	37.77778009367107	40.0	35.0	41.0	35.0	41.0
33	37.666545695299284	40.0	35.0	41.0	35.0	41.0
34	37.58779823937618	40.0	35.0	41.0	35.0	41.0
35	37.53370687329884	39.0	35.0	41.0	35.0	41.0
36	37.43856084941517	39.0	35.0	41.0	34.0	41.0
37	37.379729530909	39.0	35.0	41.0	34.0	41.0
38	37.34391015423849	39.0	35.0	41.0	34.0	41.0
39	37.220648095927025	39.0	35.0	41.0	34.0	41.0
40	37.14463475638165	38.0	35.0	41.0	34.0	41.0
41	37.095510164046985	38.0	35.0	41.0	34.0	41.0
42	37.001848900223145	38.0	35.0	41.0	34.0	41.0
43	37.02083323116157	38.0	35.0	41.0	34.0	41.0
44	36.97460704739952	38.0	35.0	41.0	34.0	41.0
45	36.95425198008877	38.0	35.0	41.0	34.0	41.0
46	36.90563007282803	38.0	35.0	41.0	34.0	41.0
47	36.817617272749565	37.0	35.0	41.0	34.0	41.0
48	36.79219857286481	37.0	35.0	41.0	34.0	41.0
49	36.71264804688458	37.0	35.0	41.0	34.0	41.0
50	36.60609597606729	37.0	35.0	40.0	34.0	41.0
51	36.517466467227386	36.0	35.0	40.0	34.0	41.0
52	36.208945342193665	36.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	6.0
14	3.0
15	7.0
16	14.0
17	33.0
18	58.0
19	122.0
20	211.0
21	370.0
22	567.0
23	963.0
24	1662.0
25	2614.0
26	3757.0
27	4334.0
28	4534.0
29	5207.0
30	6099.0
31	7486.0
32	9668.0
33	13983.0
34	45910.0
35	195257.0
36	32041.0
37	46878.0
38	83358.0
39	347738.0
40	2737.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.848287192565166	22.142664476104066	24.540840097104045	21.468208234226722
2	26.411932027169517	24.491797650866825	27.035874549422523	22.060395772541135
3	26.57463034256149	24.737377700399694	26.01088742306466	22.677104533974155
4	26.64083764498173	27.220028935043278	22.613962384443738	23.525171035531255
5	24.732718668007163	35.63926828670214	20.021210857997595	19.606802187293102
6	87.76734263505064	2.791128221475687	6.846202888600084	2.595326254873593
7	88.68983104877272	2.529364164684534	4.992153208602045	3.7886515779407075
8	79.96701895490547	4.833991319487016	9.473038915181089	5.725950810426425
9	54.50700080920037	19.7954929991908	12.633579362938624	13.063926828670214
10	45.43329001250582	20.44236286505971	21.279149603982248	12.84519751845222
11	43.23005811529879	17.74380226085677	21.58333537676859	17.442804247075845
12	21.461464897869106	36.4544763492803	24.331551457786716	17.752507295063875
13	14.404869914911355	38.268311223363824	27.82030847698683	19.50651038473799
14	13.649003212280228	24.342463402074497	44.244623721831246	17.763909663814033
15	14.988229812903068	17.34643584021971	47.780706701650274	19.884627645226942
16	15.611314092346925	19.495843652681398	27.845074912336624	37.047767342635055
17	20.304921409479903	22.266006228390673	31.515288982614454	25.91378337951497
18	25.602241239793038	27.30401412422452	25.128613815257104	21.96513082072534
19	30.224737009882052	22.459601284912093	24.04955739192271	23.266104313283144
20	21.504254432211077	29.192148304357424	26.591304774282143	22.71229248914936
21	21.085064122998457	24.412103675731345	25.34489100316324	29.15794119810696
22	19.9436011868272	29.229788381844486	22.61898923518305	28.207621196145265
23	21.08837448811947	21.371717221254997	36.12552414114416	21.41438414948138
24	19.43601186827199	21.91939873960913	33.197567494666636	25.44702189745224
25	18.082195139893578	31.526813957480197	26.833574458694	23.557416443932222
26	18.794291459257987	29.784826267134207	27.04457958362963	24.37630268997818
27	19.725607513302766	26.323410411711336	35.777077560628726	18.173904514357176
28	17.332703955273292	24.335597459601285	32.47014541085309	25.861553174272334
29	22.315048674627892	25.869399965670286	28.895686716853437	22.91986464284839
30	27.475417473823594	24.027243078884773	25.39172653931978	23.10561290797185
31	29.37372796155072	24.608886491258183	24.072729947769794	21.9446555994213
32	28.764988597631252	25.6364483460435	24.442387386282828	21.156175670042423
33	19.099948505431453	25.78664083764498	29.36514553345921	25.74826512346436
34	20.720556141340328	22.92636276697482	30.705475589122383	25.64760550256247
35	31.114489590740785	21.603442779725853	24.658174149726587	22.62389347980677
36	22.657242343248083	28.028984085726194	28.734827493195358	20.578946077830363
37	22.30217503249062	27.469041955812756	27.629901179470835	22.598881832225793
38	22.374144822343737	27.36188421078443	26.306000343297125	23.957970623574703
39	27.728966920870015	23.362227507908095	25.775115862779234	23.133689708442656
40	17.631740271204727	20.94639660626272	32.277040778794046	29.144822343738507
41	22.740737107966947	24.52343002868983	24.606556975061917	28.129275888281306
42	23.772344964566834	22.724185282361884	27.106250459772934	26.397219293298352
43	23.206885559451706	26.808685417228613	26.83210318530688	23.1523258380128
44	19.707216595963807	29.48027267600108	25.325519236899535	25.48699149113558
45	23.09923738996101	26.563350579926926	26.520438439469363	23.8169735906427
46	23.52014418479194	23.439837179078495	28.5684509943356	24.471567641793975
47	24.032515141855278	19.662587969887937	32.384811554400336	23.920085333856452
48	20.232583801280008	20.160246193080113	30.10997768568696	29.49719231995292
49	20.250239081925407	24.923003359407566	31.048772712782913	23.777984845884113
50	19.487261224589883	27.191461710110097	27.38873495009931	25.932542115200707
51	21.37944140653736	22.257301194183565	29.418233981511	26.945023417768077
52	24.280792525931194	20.02047522130404	30.930580417351216	24.76815183541355
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	123.0
1	258.5
2	394.0
3	1481.0
4	2568.0
5	1757.5
6	947.0
7	1019.0
8	1091.0
9	1144.5
10	1198.0
11	1173.0
12	1148.0
13	1129.0
14	1084.5
15	1059.0
16	1049.0
17	1039.0
18	998.0
19	957.0
20	1076.5
21	1196.0
22	1200.5
23	1205.0
24	1544.5
25	1884.0
26	2545.0
27	3206.0
28	4697.5
29	6189.0
30	5894.5
31	5600.0
32	6827.5
33	8055.0
34	10615.0
35	13175.0
36	14073.0
37	14971.0
38	16101.5
39	19742.5
40	22253.0
41	31330.0
42	40407.0
43	55481.0
44	70555.0
45	80120.5
46	89686.0
47	88759.5
48	87833.0
49	85627.0
50	83421.0
51	77024.0
52	70627.0
53	61390.0
54	52153.0
55	45978.0
56	39803.0
57	36467.0
58	33131.0
59	32451.5
60	31772.0
61	32055.0
62	32338.0
63	30108.0
64	23081.0
65	18284.0
66	14527.5
67	10771.0
68	8842.5
69	6914.0
70	5937.5
71	4961.0
72	3976.0
73	2991.0
74	2680.5
75	2370.0
76	2161.0
77	1952.0
78	1252.5
79	553.0
80	477.0
81	401.0
82	278.5
83	156.0
84	102.5
85	49.0
86	27.0
87	5.0
88	4.0
89	3.0
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	815620.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.60855118717057
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.909905642272	16.031702313907417
2	11.968931983715896	5.412004228192076
3	4.417599317713409	2.9962656089680015
4	2.1491374601227444	1.9435553710180327
5	1.1781511291793243	1.3318145055136776
6	0.7783721310735754	1.0558719700826382
7	0.5046918404258348	0.7987245915610366
8	0.37276177351570816	0.6742082909720296
9	0.29268887843779073	0.5955544341068703
>10	5.453780659419308	34.26049598800386
>50	1.7560370990930545	24.981934514675125
>100	0.20761565207474703	7.157812243180503
>500	0.0070654541281981965	1.1480368019644895
>1k	0.003260978828399168	1.612019137854237
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	4103	0.5030528922782668	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCTT	3161	0.3875579313896177	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	2091	0.25636938770505874	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1396	0.17115813736789193	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1262	0.15472891787842377	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	1106	0.13560236384590865	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	931	0.11414629361712561	No Hit
ACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	904	0.11083592849611339	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT	853	0.10458301660086805	No Hit
TCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	853	0.10458301660086805	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.39601775336553785	0.0
2	0.0	0.0	0.0	1.3514872121821437	0.0
3	0.0	0.0	0.0	2.0898212402834653	0.0
4	0.0	0.0	0.0	2.5299771952624996	0.0
5	0.0	0.0	0.0	4.815109977685687	0.0
6	0.0	0.0	0.0	5.190775115862779	0.0
7	0.0	0.0	0.0	6.578308526029279	0.0
8	0.0	0.0	0.0	8.197322282435447	0.0
9	0.0	0.0	0.0	9.471935460140752	0.0
10	0.0	0.0	0.0	10.320614992275814	0.0
11	0.0	0.0	0.0	11.15249748657463	0.0
12	0.0	0.0	0.0	11.913391039945072	0.0
13	0.0	0.0	0.0	12.225546210244968	0.0
14	0.0	0.0	0.0	12.459723891027684	0.0
15	0.0	0.0	0.0	13.254456732301808	0.0
16	0.0	0.0	0.0	14.085971408253844	0.0
17	0.0	0.0	0.0	15.270469090998258	0.0
18	0.0	0.0	0.0	15.784066109217529	0.0
19	0.0	0.0	0.0	16.350996787719772	0.0
20	0.0	0.0	0.0	17.0521811627964	0.0
21	0.0	0.0	0.0	17.650008582428093	0.0
22	1.2260611559304578E-4	0.0	0.0	18.373016846080283	0.0
23	1.2260611559304578E-4	0.0	0.0	18.881096589097865	0.0
24	2.4521223118609156E-4	0.0	0.0	19.308501508055222	0.0
25	2.4521223118609156E-4	0.0	0.0	19.71690247909566	0.0
26	2.4521223118609156E-4	0.0	0.0	20.08165567298497	0.0
27	2.4521223118609156E-4	0.0	0.0	20.476447365194577	0.0
28	2.4521223118609156E-4	0.0	0.0	20.856894141879796	0.0
29	2.4521223118609156E-4	0.0	0.0	21.35663666903705	0.0
30	2.4521223118609156E-4	0.0	0.0	21.83921434001128	0.0
31	2.4521223118609156E-4	0.0	0.0	22.271891321939137	0.0
32	2.4521223118609156E-4	0.0	0.0	22.67943405017042	0.0
33	2.4521223118609156E-4	0.0	0.0	23.060739069664795	0.0
34	2.4521223118609156E-4	0.0	0.0	23.59432088472573	0.0
35	2.4521223118609156E-4	0.0	0.0	24.017679801868518	0.0
36	2.4521223118609156E-4	0.0	0.0	24.477943159804813	0.0
37	2.4521223118609156E-4	0.0	0.0	24.897991711826585	0.0
38	2.4521223118609156E-4	0.0	0.0	25.266668301414875	0.0
39	2.4521223118609156E-4	0.0	0.0	25.740418332066405	0.0
40	2.4521223118609156E-4	0.0	0.0	26.198597386037616	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAC	25	3.4162775E-5	46.0	8
AACGTCA	20	6.3103705E-4	46.0	43
CTCACGA	25	3.4162775E-5	46.0	23
ACCGGGT	25	3.4162775E-5	46.0	3
TACCAGG	95	0.0	46.0	1
CCGATAG	40	5.6079443E-9	46.0	11
ACGGGTC	25	3.4162775E-5	46.0	4
ACGGGTA	45	3.092282E-10	46.0	4
AACGGCG	20	6.3103705E-4	46.0	37
CTAACGG	55	1.8189894E-12	46.0	1
GCCGAGT	20	6.3103705E-4	46.0	13
TTCCACG	20	6.3103705E-4	46.0	42
ACAATCC	30	1.8607334E-6	46.0	41
GTCGTTT	20	6.3103705E-4	46.0	8
CAAATCG	25	3.4162775E-5	46.0	43
CTACCGG	40	5.6079443E-9	46.0	1
TCACTAG	20	6.3103705E-4	46.0	43
TCCAACG	20	6.3103705E-4	46.0	12
ACGTCGA	20	6.3103705E-4	46.0	28
CGTAATG	20	6.3103705E-4	46.0	43
>>END_MODULE