FastQCFastQC Report
Thu 26 May 2016
SRR1544805_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544805_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences567119
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT42280.7455225446511226Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCTT34040.6002267601685007Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT21450.3782274972272133Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCTTG16730.29499981485367266Illumina Single End Adapter 1 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA16350.28829928110326053No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC9190.16204711885865222No Hit
ACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC8990.158520522147909No Hit
TCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT8750.1542886060950171Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC7140.12589950257353397No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA6630.11690668096113867No Hit
AGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC6620.11673035112560151No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG6540.1153197124413042No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC6080.10720854000659473No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5910.10421093280246299No Hit
ACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT5890.10385827313138865Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGA206.3087355E-446.00000435
CCGTAGG405.6024874E-946.0000041
ACAATCG206.3087355E-446.00000436
GTCCGCA206.3087355E-446.00000430
CGTAAGT206.3087355E-446.00000411
ACGTAGG1100.046.0000041
ACGTAAG206.3087355E-446.00000410
CGTCCTA206.3087355E-446.00000427
CCAAACG206.3087355E-446.00000415
TCGAAGA206.3087355E-446.00000426
CCGCAAT206.3087355E-446.00000432
TCGAAAC206.3087355E-446.00000424
TTGGGCA405.6024874E-946.0000044
AATCGAA206.3087355E-446.00000422
CTAGCGG1450.046.0000041
TCACGAC301.8597239E-646.024
GACAGTA253.4149503E-546.08
CGACGGT301.8597239E-646.027
ACGATAC253.4149503E-546.030
GTTCGGA253.4149503E-546.017
Produced by FastQC (version 0.11.5)