##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544805_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	567119
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91749174335545	34.0	33.0	34.0	31.0	34.0
2	33.26280198688459	34.0	33.0	34.0	31.0	34.0
3	33.35571017722912	34.0	34.0	34.0	31.0	34.0
4	36.59840879956411	37.0	37.0	37.0	35.0	37.0
5	36.59557165251032	37.0	37.0	37.0	35.0	37.0
6	36.53064524376718	37.0	37.0	37.0	35.0	37.0
7	36.408714925791585	37.0	37.0	37.0	35.0	37.0
8	36.44580943329354	37.0	37.0	37.0	35.0	37.0
9	38.29360151925786	39.0	39.0	39.0	37.0	39.0
10	38.122690299566756	39.0	39.0	39.0	35.0	39.0
11	37.394585616069996	39.0	35.0	39.0	35.0	39.0
12	37.3393220823143	39.0	35.0	39.0	35.0	39.0
13	37.31996988286409	39.0	35.0	39.0	35.0	39.0
14	38.540567323612855	41.0	35.0	41.0	35.0	41.0
15	38.594524253287226	41.0	35.0	41.0	35.0	41.0
16	38.584776739978736	41.0	35.0	41.0	35.0	41.0
17	38.677727249483794	41.0	36.0	41.0	35.0	41.0
18	38.66652501503212	41.0	36.0	41.0	35.0	41.0
19	38.59598779092219	41.0	35.0	41.0	35.0	41.0
20	38.519157354981935	40.0	35.0	41.0	35.0	41.0
21	38.44692736445085	40.0	35.0	41.0	35.0	41.0
22	38.43142091871371	40.0	35.0	41.0	35.0	41.0
23	38.40562386377462	40.0	35.0	41.0	35.0	41.0
24	38.33393167924192	40.0	35.0	41.0	35.0	41.0
25	38.27120410354793	40.0	35.0	41.0	35.0	41.0
26	38.19895471673494	40.0	35.0	41.0	35.0	41.0
27	38.17497385910188	40.0	35.0	41.0	35.0	41.0
28	38.23379572893872	40.0	35.0	41.0	35.0	41.0
29	38.292076266180466	40.0	35.0	41.0	35.0	41.0
30	38.173094183055056	40.0	35.0	41.0	35.0	41.0
31	38.1793785783936	40.0	35.0	41.0	35.0	41.0
32	38.15402940123678	40.0	35.0	41.0	35.0	41.0
33	38.04454267975504	40.0	35.0	41.0	35.0	41.0
34	38.010516311391434	40.0	35.0	41.0	35.0	41.0
35	37.94416515757716	40.0	35.0	41.0	35.0	41.0
36	37.891687635222944	40.0	35.0	41.0	35.0	41.0
37	37.83889977235818	40.0	35.0	41.0	35.0	41.0
38	37.796450127750965	40.0	35.0	41.0	35.0	41.0
39	37.75017059911588	40.0	35.0	41.0	35.0	41.0
40	37.69662451795831	40.0	35.0	41.0	35.0	41.0
41	37.66957375788856	40.0	35.0	41.0	35.0	41.0
42	37.5991440949783	39.0	35.0	41.0	35.0	41.0
43	37.52014127546423	39.0	35.0	41.0	35.0	41.0
44	37.36971076617077	39.0	35.0	41.0	35.0	41.0
45	37.345431911115654	39.0	35.0	41.0	35.0	41.0
46	37.290473427975435	39.0	35.0	41.0	35.0	41.0
47	37.21347195209471	38.0	35.0	41.0	35.0	41.0
48	37.157291503194216	38.0	35.0	41.0	34.0	41.0
49	37.09499769889565	38.0	35.0	41.0	34.0	41.0
50	36.992664678841656	37.0	35.0	41.0	34.0	41.0
51	36.90304151333318	37.0	35.0	41.0	34.0	41.0
52	36.40345324349916	36.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	3.0
13	4.0
14	3.0
15	6.0
16	14.0
17	29.0
18	40.0
19	101.0
20	146.0
21	226.0
22	338.0
23	513.0
24	873.0
25	1400.0
26	2007.0
27	2321.0
28	2456.0
29	2730.0
30	3242.0
31	4194.0
32	5533.0
33	8050.0
34	27540.0
35	129447.0
36	20597.0
37	31784.0
38	58645.0
39	263227.0
40	1648.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.223781957578566	22.004905496024644	23.559605655955803	23.211706890440983
2	26.033336918706656	24.50314660591516	26.727018491709853	22.73649798366833
3	26.690870875424732	24.46029845587963	25.90320550008023	22.945625168615404
4	26.674119541048704	26.529000086401624	22.997113480592258	23.799766891957418
5	24.443899781174675	34.88985556823171	20.265059008779463	20.401185641814152
6	85.50621650835186	2.9067973388301223	8.018070281545848	3.5689158712721674
7	86.56296121272608	2.632957104240909	5.968059613590798	4.836022069442216
8	76.46014328562435	5.1160338482752294	11.330249912275907	7.0935729538245065
9	52.31318294749426	19.31270156704325	13.797280641276346	14.57683484418614
10	43.14103389235769	20.0427070861671	23.54920219565911	13.267056825816098
11	40.55780180173826	17.770344495599687	22.571100597934475	19.10075310472758
12	20.87110465352069	34.58674458094333	25.93353423179262	18.60861653374336
13	14.264731035285363	36.60148928179095	28.91897467727232	20.21480500565137
14	13.551124190866467	24.303012242580483	42.90369393372467	19.242169632828386
15	14.657241249191086	17.664370264441853	46.80516787481992	20.873220611547136
16	15.032823798885243	20.833193738880198	27.972436120108828	36.161546342125725
17	19.176398604173023	22.779875123210473	31.61505786263553	26.428668409980975
18	24.58901923582176	27.561587603307242	25.746977265794303	22.102415895076692
19	28.626619809951702	22.660852484222886	24.226485093957354	24.486042611868054
20	20.630061768341392	29.92123346246555	26.750646689671832	22.69805807952123
21	20.728630146406662	24.419566263870546	25.55777535226293	29.294028237459862
22	19.438424739781247	29.564694535009405	22.539713887208855	28.457166838000493
23	20.17689409101088	21.81623257199988	35.85420343878445	22.152669898204785
24	20.20475420502575	22.236426570084937	32.12623805585776	25.432581169031543
25	18.03431025939882	30.772024918932356	27.02730820162964	24.16635662003918
26	19.152594076375504	28.25227156910631	26.683641352167708	25.91149300235048
27	21.148295154985107	25.471373732849717	34.68954487506149	18.690786237103676
28	17.67636069325838	23.851255203934272	31.6490895208942	26.823294581913142
29	23.532098201612005	24.768699338234125	28.22670374295342	23.472498717200445
30	27.040532939294927	23.520460432466557	25.834965853727347	23.60404077451117
31	29.321535691803664	23.593813644050012	23.702961812247516	23.381688851898808
32	29.229315187817722	24.533299007792014	24.85298499962089	21.384400804769367
33	19.269500757336644	24.558690504109368	30.15469416471675	26.017114573837237
34	21.354424732728052	21.635141830903216	31.334517094295904	25.67591634207283
35	31.27209633251575	20.633764694887667	25.18131115339109	22.912827819205493
36	23.033613756548448	27.527379615213032	28.563670058664936	20.875336569573584
37	22.634050349221237	26.381235684221476	28.99038826066487	21.994325705892415
38	22.606366565041906	26.58119371772062	25.98713850179592	24.825301215441556
39	28.31927690661043	22.43567928424193	26.214780319474396	23.030263489673246
40	18.10907410966658	20.39025319201085	32.282289960308155	29.21838273801442
41	22.658736526196442	24.53188836910772	24.602420303322585	28.206954801373257
42	23.384333799431865	21.840742419139545	27.730158926080772	27.044764855347818
43	24.40775216488956	25.55213279752574	26.620691600880942	23.41942343670376
44	19.961771691655546	27.441683315141972	26.406627180538827	26.189917812663655
45	22.956204958747637	25.09103027759606	27.07862018377096	24.874144579885353
46	24.0706095193425	21.649424547581724	29.067620728630146	25.21234520444563
47	23.238332695607095	18.80063972464333	33.47216369051292	24.48886388923665
48	20.372443878621592	18.90555597678794	30.084691220008498	30.63730892458197
49	20.47348087438439	23.122660323494717	31.851163512419795	24.552695289701106
50	19.80289850983656	25.05946723703491	28.626090820445093	26.51154343268344
51	21.08464008435619	20.913071154378535	29.689536058569715	28.312752702695555
52	23.74245969540784	19.262800223586233	32.57358684861555	24.42115323239038
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	90.0
1	140.5
2	191.0
3	696.5
4	1202.0
5	875.0
6	548.0
7	559.0
8	570.0
9	588.0
10	606.0
11	629.0
12	652.0
13	642.5
14	616.0
15	599.0
16	610.5
17	622.0
18	653.0
19	684.0
20	723.0
21	762.0
22	728.5
23	695.0
24	917.0
25	1139.0
26	1584.0
27	2029.0
28	2823.0
29	3617.0
30	3514.0
31	3411.0
32	4179.5
33	4948.0
34	6524.5
35	8101.0
36	8747.0
37	9393.0
38	10281.0
39	12655.0
40	14141.0
41	19965.5
42	25790.0
43	35766.0
44	45742.0
45	53179.5
46	60617.0
47	63613.5
48	66610.0
49	66554.0
50	66498.0
51	59908.0
52	53318.0
53	45735.0
54	38152.0
55	33341.0
56	28530.0
57	25184.0
58	21838.0
59	21237.5
60	20637.0
61	20885.0
62	21133.0
63	20297.0
64	15793.0
65	12125.0
66	9623.5
67	7122.0
68	5860.0
69	4598.0
70	4005.5
71	3413.0
72	2728.5
73	2044.0
74	1849.0
75	1654.0
76	1443.5
77	1233.0
78	788.5
79	344.0
80	328.5
81	313.0
82	208.0
83	103.0
84	69.0
85	35.0
86	20.0
87	5.0
88	3.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	567119.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.374498264702407
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.31316187958544	18.808488642435677
2	11.52105888842625	6.077242953206646
3	3.998244942203117	3.1635511286997384
4	1.8935718656436014	1.9976803153802587
5	1.0758844278088413	1.4187955987132312
6	0.7168125354908614	1.1343342584052394
7	0.5327207920660609	0.9835170524142048
8	0.43790610600734114	0.9239643066394566
9	0.37587918313858304	0.8922262377083684
>10	7.626217901093861	49.31805900303272
>50	0.4005267317757872	7.008310433697945
>100	0.09593856389269274	4.404723196052288
>500	0.00872168762660843	1.553765089966462
>1k	0.003354495241003243	2.31534178364778
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	4228	0.7455225446511226	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCTT	3404	0.6002267601685007	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	2145	0.3782274972272133	Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCTTG	1673	0.29499981485367266	Illumina Single End Adapter 1 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1635	0.28829928110326053	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	919	0.16204711885865222	No Hit
ACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	899	0.158520522147909	No Hit
TCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	875	0.1542886060950171	Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	714	0.12589950257353397	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	663	0.11690668096113867	No Hit
AGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	662	0.11673035112560151	No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	654	0.1153197124413042	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	608	0.10720854000659473	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	591	0.10421093280246299	No Hit
ACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	589	0.10385827313138865	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6099249011230448	0.0
2	0.0	0.0	0.0	2.0170369887096005	0.0
3	0.0	0.0	0.0	3.0434529613714227	0.0
4	0.0	0.0	0.0	3.678593028976282	0.0
5	0.0	0.0	0.0	6.786759039989843	0.0
6	0.0	0.0	0.0	7.289828060777368	0.0
7	0.0	0.0	0.0	9.172678044643188	0.0
8	0.0	0.0	0.0	11.272413726219717	0.0
9	0.0	0.0	0.0	12.96676711589631	0.0
10	0.0	0.0	0.0	14.100567958400266	0.0
11	0.0	0.0	0.0	15.18905203317117	0.0
12	1.763298355371624E-4	0.0	0.0	16.14652304013796	0.0
13	1.763298355371624E-4	0.0	0.0	16.560898153650292	0.0
14	1.763298355371624E-4	0.0	0.0	16.863832811103137	0.0
15	1.763298355371624E-4	0.0	0.0	17.79273838471291	0.0
16	1.763298355371624E-4	0.0	0.0	18.78688599747143	0.0
17	1.763298355371624E-4	0.0	0.0	20.084673587024945	0.0
18	1.763298355371624E-4	0.0	0.0	20.6951274776546	0.0
19	1.763298355371624E-4	0.0	0.0	21.35812765927433	0.0
20	1.763298355371624E-4	0.0	0.0	22.181235331561805	0.0
21	1.763298355371624E-4	0.0	0.0	22.905774625784005	0.0
22	3.526596710743248E-4	0.0	0.0	23.718831497445862	0.0
23	3.526596710743248E-4	0.0	0.0	24.298780326527588	0.0
24	3.526596710743248E-4	0.0	0.0	24.767465029385367	0.0
25	3.526596710743248E-4	0.0	0.0	25.233152125039013	0.0
26	3.526596710743248E-4	0.0	0.0	25.632539202530687	0.0
27	3.526596710743248E-4	0.0	0.0	26.060668043214918	0.0
28	3.526596710743248E-4	0.0	0.0	26.46358171741733	0.0
29	3.526596710743248E-4	0.0	0.0	26.99927175777923	0.0
30	3.526596710743248E-4	0.0	0.0	27.51944477261386	0.0
31	3.526596710743248E-4	0.0	0.0	28.007702087216263	0.0
32	3.526596710743248E-4	0.0	0.0	28.442178801979832	0.0
33	3.526596710743248E-4	0.0	0.0	28.827988482135144	0.0
34	3.526596710743248E-4	0.0	0.0	29.39841550009786	0.0
35	3.526596710743248E-4	0.0	0.0	29.814377582130028	0.0
36	3.526596710743248E-4	0.0	0.0	30.30210590722582	0.0
37	3.526596710743248E-4	0.0	0.0	30.72847144955468	0.0
38	3.526596710743248E-4	0.0	0.0	31.117102407078583	0.0
39	3.526596710743248E-4	0.0	0.0	31.589313706647104	0.0
40	3.526596710743248E-4	0.0	0.0	32.055353461971826	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTCGA	20	6.3087355E-4	46.000004	35
CCGTAGG	40	5.6024874E-9	46.000004	1
ACAATCG	20	6.3087355E-4	46.000004	36
GTCCGCA	20	6.3087355E-4	46.000004	30
CGTAAGT	20	6.3087355E-4	46.000004	11
ACGTAGG	110	0.0	46.000004	1
ACGTAAG	20	6.3087355E-4	46.000004	10
CGTCCTA	20	6.3087355E-4	46.000004	27
CCAAACG	20	6.3087355E-4	46.000004	15
TCGAAGA	20	6.3087355E-4	46.000004	26
CCGCAAT	20	6.3087355E-4	46.000004	32
TCGAAAC	20	6.3087355E-4	46.000004	24
TTGGGCA	40	5.6024874E-9	46.000004	4
AATCGAA	20	6.3087355E-4	46.000004	22
CTAGCGG	145	0.0	46.000004	1
TCACGAC	30	1.8597239E-6	46.0	24
GACAGTA	25	3.4149503E-5	46.0	8
CGACGGT	30	1.8597239E-6	46.0	27
ACGATAC	25	3.4149503E-5	46.0	30
GTTCGGA	25	3.4149503E-5	46.0	17
>>END_MODULE