FastQCFastQC Report
Thu 26 May 2016
SRR1544804_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544804_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences788059
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT23090.29299836687354625Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCTT20410.2589907608440485Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT14600.18526531642935362Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13070.16585052641997616No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC11830.150115663928716No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA11770.14935429961462277No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA9580.12156450215021972No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC9380.11902662110324226No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCA351.01901605E-746.00000416
ACTACGG700.046.0000041
ATGACGG351.01901605E-746.0000041
AACCTAG351.01901605E-746.00000418
ACGTAGG700.046.0000041
TCACGAC253.4161712E-546.024
AACGTTC206.3102413E-446.039
CGAACGT206.3102413E-446.037
ACACGCG253.4161712E-546.035
ATGATTC206.3102413E-446.08
TCCGATT206.3102413E-446.020
CTATCGG253.4161712E-546.01
CGTGCCT206.3102413E-446.039
GCTATCG206.3102413E-446.017
ATTAACG206.3102413E-446.041
ATTCGCG206.3102413E-446.013
GTAATCG206.3102413E-446.034
CTAACGG850.046.01
CCGTACC206.3102413E-446.044
TGATTCG253.4161712E-546.045
Produced by FastQC (version 0.11.5)