##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544804_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	788059
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.96381996779429	34.0	33.0	34.0	31.0	34.0
2	33.2934983294398	34.0	33.0	34.0	31.0	34.0
3	33.35368798529044	34.0	34.0	34.0	31.0	34.0
4	36.61892700927215	37.0	37.0	37.0	35.0	37.0
5	36.617976572820055	37.0	37.0	37.0	35.0	37.0
6	36.70309964101673	37.0	37.0	37.0	36.0	37.0
7	36.684528696455466	37.0	37.0	37.0	36.0	37.0
8	36.58552088105078	37.0	37.0	37.0	35.0	37.0
9	38.384766876591726	39.0	39.0	39.0	37.0	39.0
10	38.12582941124967	39.0	39.0	39.0	35.0	39.0
11	37.30281615970378	39.0	35.0	39.0	35.0	39.0
12	37.1965157431106	39.0	35.0	39.0	35.0	39.0
13	37.17308475634439	39.0	35.0	39.0	35.0	39.0
14	38.3946734952586	40.0	35.0	41.0	35.0	41.0
15	38.47022875190817	40.0	35.0	41.0	35.0	41.0
16	38.47394294082042	40.0	35.0	41.0	35.0	41.0
17	38.55417551223957	40.0	36.0	41.0	35.0	41.0
18	38.53329002016346	40.0	36.0	41.0	35.0	41.0
19	38.42641477351315	40.0	35.0	41.0	35.0	41.0
20	38.33948346507051	40.0	35.0	41.0	35.0	41.0
21	38.27340592519088	40.0	35.0	41.0	35.0	41.0
22	38.25175526197911	40.0	35.0	41.0	35.0	41.0
23	38.20968734574442	40.0	35.0	41.0	35.0	41.0
24	38.15866197835441	40.0	35.0	41.0	35.0	41.0
25	38.12462391775235	40.0	35.0	41.0	35.0	41.0
26	38.05476493511273	40.0	35.0	41.0	35.0	41.0
27	38.06086346326861	40.0	35.0	41.0	35.0	41.0
28	38.09401453444475	40.0	35.0	41.0	35.0	41.0
29	38.10941566557834	40.0	35.0	41.0	35.0	41.0
30	38.01377815620404	40.0	35.0	41.0	35.0	41.0
31	37.939928355618044	40.0	35.0	41.0	35.0	41.0
32	37.8671723817633	40.0	35.0	41.0	35.0	41.0
33	37.78553001742255	40.0	35.0	41.0	35.0	41.0
34	37.69286563569479	40.0	35.0	41.0	35.0	41.0
35	37.59030478682433	40.0	35.0	41.0	35.0	41.0
36	37.51146043633789	39.0	35.0	41.0	35.0	41.0
37	37.46059876227541	39.0	35.0	41.0	35.0	41.0
38	37.41013299765627	39.0	35.0	41.0	35.0	41.0
39	37.27823043706118	39.0	35.0	41.0	34.0	41.0
40	37.21707004171007	39.0	35.0	41.0	34.0	41.0
41	37.18204220749969	38.0	35.0	41.0	34.0	41.0
42	37.093896522976074	38.0	35.0	41.0	34.0	41.0
43	37.08263086900854	38.0	35.0	41.0	34.0	41.0
44	37.05291862665105	38.0	35.0	41.0	34.0	41.0
45	37.03714315806304	38.0	35.0	41.0	34.0	41.0
46	36.970735693647306	37.0	35.0	41.0	34.0	41.0
47	36.884002339926326	37.0	35.0	41.0	34.0	41.0
48	36.85768451346917	37.0	35.0	41.0	34.0	41.0
49	36.79571834088564	37.0	35.0	41.0	34.0	41.0
50	36.711818531353615	37.0	35.0	41.0	34.0	41.0
51	36.61509353995069	36.0	35.0	41.0	34.0	41.0
52	36.36183458345124	36.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	2.0
15	8.0
16	14.0
17	37.0
18	60.0
19	125.0
20	186.0
21	298.0
22	521.0
23	824.0
24	1439.0
25	2382.0
26	3386.0
27	3914.0
28	4009.0
29	4351.0
30	5119.0
31	6403.0
32	8239.0
33	12396.0
34	43703.0
35	193021.0
36	29144.0
37	47635.0
38	78224.0
39	339440.0
40	3177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.327453908907835	22.071063207196413	24.656402629752343	19.945080254143406
2	26.59470927938137	24.15593248728839	26.771472694303345	22.4778855390269
3	26.799008703663052	24.994067703052693	25.7691365747996	22.437787018484656
4	25.96303068678868	27.504032058513385	22.866054445162103	23.66688280953583
5	25.337823690865786	35.56142370050973	20.094180765653334	19.006571842971148
6	88.9480356166226	3.0844137304440404	5.66252019201608	2.3050304609172665
7	90.06584532376382	2.6229000620511913	4.373276620151537	2.9379779940334414
8	81.7021314393973	4.997468463655641	8.208522458343854	5.091877638603201
9	55.69976359638047	20.27601994266927	11.869162080504125	12.155054380446135
10	46.58851684962674	20.490851573295906	19.393091126425812	13.527540450651538
11	43.62998201911278	18.26106928542152	20.887395486886135	17.22155320857956
12	21.382282291046735	37.87152992352095	23.049035668649175	17.697152116783137
13	15.039609978440701	40.42971401887422	25.931941643963206	18.59873435872187
14	13.484142684748225	24.494993395164574	44.32891445945037	17.69194946063683
15	16.094607129669225	17.653246774670425	47.44987367697089	18.802272418689466
16	17.197316444580927	19.227748176215233	26.82032690445766	36.75460847474618
17	21.381140244575597	23.06971939918204	31.182817530159546	24.36632282608282
18	26.80357688954761	25.820909348157944	24.51669227811623	22.858821484178215
19	31.088662143316682	22.61873793713415	23.94960275816912	22.342997161380048
20	22.531181041013426	28.24065203239859	26.19550059069181	23.03266633589617
21	22.58929851698921	23.866487153880612	24.784311834520004	28.759902494610174
22	20.45912806020869	29.043637595662254	22.56366591841474	27.933568425714316
23	21.075198684362466	21.536966140860013	36.59484886283895	20.792986311938574
24	19.157956447423352	21.764867858878585	33.81739184502683	25.25978384867123
25	18.455470973619995	31.860939345911916	26.209205148345493	23.474384532122595
26	18.239370402469866	30.676002685078146	28.224409593697935	22.860217318754053
27	18.51016231018236	26.377720449864796	37.01918257389358	18.092934666059268
28	17.106206514994433	25.179713701639088	33.23812049605423	24.475959287312243
29	19.852447595928734	27.63054542870521	30.57207645620442	21.944930519161638
30	27.99155900763775	24.95244645388226	23.529710338946703	23.526284199533283
31	28.751654381207498	27.544638155265023	24.238413621315154	19.465293842212322
32	28.000187803197473	27.0803328177205	24.899531634052778	20.019947745029242
33	19.452477542925088	26.69343285210879	27.514183582701296	26.33990602226483
34	21.407153525307116	25.12045417919217	29.512130436934292	23.96026185856643
35	31.20223232016892	22.280311499519705	24.84522097964746	21.67223520066391
36	22.34198200896126	28.264635008292526	28.032672680598786	21.360710302147428
37	23.649625218416386	28.99579853792673	23.999091438585182	23.355484805071704
38	22.343758525694142	27.877608148628465	26.108578164832835	23.670055160844555
39	26.634554011818913	23.385431801426034	25.94133180383702	24.03868238291803
40	17.827599202597774	21.0036304388377	31.530761021700155	29.638009336864375
41	22.148849261286273	24.783169788048866	24.916916119224574	28.151064831440287
42	23.244833191423485	23.074033796961903	27.964403680435097	25.716729331179515
43	21.937189981968356	28.056528762440376	27.768859945765485	22.237421309825788
44	19.290053155918528	31.913981059793745	24.225851110132616	24.570114674155107
45	23.517528509921213	26.981736139045427	25.540727280571634	23.96000807046173
46	23.919655761814788	24.404644829892177	27.565068097693192	24.110631310599842
47	24.42659750094853	21.10946007849666	31.46985187657269	22.99409054398211
48	21.406138372888325	20.112707297296268	30.91621312617456	27.564941203640842
49	21.27264582981731	26.845578820875087	28.94935531476704	22.93242003454056
50	19.807146419240183	28.958999262745554	25.94463104919809	25.28922326881617
51	22.332211166930392	23.246990390313414	28.79327563037793	25.627522812378263
52	24.985692695597663	21.418700884070862	28.911287099062378	24.684319321269093
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	120.0
1	236.5
2	353.0
3	1363.5
4	2374.0
5	1650.5
6	927.0
7	1025.0
8	1123.0
9	1130.5
10	1138.0
11	1122.0
12	1106.0
13	1056.5
14	1031.0
15	1055.0
16	949.0
17	843.0
18	920.0
19	997.0
20	1143.5
21	1290.0
22	1461.0
23	1632.0
24	1985.0
25	2338.0
26	2562.5
27	2787.0
28	4386.5
29	5986.0
30	6742.0
31	7498.0
32	8588.5
33	9679.0
34	11236.0
35	12793.0
36	13210.0
37	13627.0
38	15994.0
39	20947.0
40	23533.0
41	33288.5
42	43044.0
43	58431.0
44	73818.0
45	78480.0
46	83142.0
47	82722.0
48	82302.0
49	78869.5
50	75437.0
51	68960.5
52	62484.0
53	54954.5
54	47425.0
55	42130.0
56	36835.0
57	34098.5
58	31362.0
59	31683.5
60	32005.0
61	31579.5
62	31154.0
63	28697.0
64	22454.5
65	18669.0
66	15189.5
67	11710.0
68	9516.5
69	7323.0
70	6860.0
71	6397.0
72	4966.5
73	3536.0
74	2719.5
75	1903.0
76	1595.5
77	1288.0
78	1131.5
79	975.0
80	660.5
81	346.0
82	196.5
83	47.0
84	45.0
85	43.0
86	22.5
87	2.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	788059.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.15449718611938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.82013594673484	15.19318863791913
2	11.332808327322462	4.794797237423466
3	4.119008874034291	2.6140668490607752
4	1.868109338374437	1.5807565496802143
5	0.9958444587559224	1.053329440028237
6	0.6351687852886901	0.8062005768660275
7	0.46196414456470086	0.6840833437397408
8	0.37189147270713346	0.6293741690339877
9	0.30242915156445954	0.5757962972193748
>10	5.7276934766312975	34.45173116927756
>50	2.127489572702934	28.488691369453395
>100	0.2272368065786124	6.984764102776822
>500	0.006612711302552211	0.938016532337081
>1k	0.003606933437755752	1.2052037251841807
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT	2309	0.29299836687354625	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCTT	2041	0.2589907608440485	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT	1460	0.18526531642935362	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1307	0.16585052641997616	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1183	0.150115663928716	No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	1177	0.14935429961462277	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	958	0.12156450215021972	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	938	0.11902662110324226	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.26355894672860786	0.0
2	0.0	0.0	0.0	0.885720485395129	0.0
3	0.0	0.0	0.0	1.4081432989154365	0.0
4	0.0	0.0	0.0	1.7196681974319181	0.0
5	0.0	0.0	0.0	3.3357908481471563	0.0
6	0.0	0.0	0.0	3.590720999316041	0.0
7	0.0	0.0	0.0	4.472380875036007	0.0
8	0.0	0.0	0.0	5.640186838802679	0.0
9	0.0	0.0	0.0	6.462840980180418	0.0
10	0.0	0.0	0.0	7.083860472375799	0.0
11	0.0	0.0	0.0	7.676328802792685	0.0
12	0.0	0.0	0.0	8.227048989986791	0.0
13	0.0	0.0	0.0	8.458249953366435	0.0
14	0.0	0.0	0.0	8.639327766068277	0.0
15	0.0	0.0	0.0	9.232684354851603	0.0
16	0.0	0.0	0.0	9.868169769014756	0.0
17	0.0	0.0	0.0	10.759981168922632	0.0
18	0.0	0.0	0.0	11.163631149444395	0.0
19	0.0	0.0	0.0	11.626667546465429	0.0
20	0.0	0.0	0.0	12.21279117426487	0.0
21	0.0	0.0	0.0	12.728107920853642	0.0
22	0.0	0.0	0.0	13.349254307101372	0.0
23	0.0	0.0	0.0	13.779044462407002	0.0
24	0.0	0.0	0.0	14.144372439119406	0.0
25	0.0	0.0	0.0	14.50741632288953	0.0
26	0.0	0.0	0.0	14.83378782553083	0.0
27	0.0	0.0	0.0	15.180589270600297	0.0
28	0.0	0.0	0.0	15.510513806707365	0.0
29	0.0	0.0	0.0	15.944745253845207	0.0
30	0.0	0.0	0.0	16.35968880502602	0.0
31	0.0	0.0	0.0	16.742528160962568	0.0
32	0.0	0.0	0.0	17.100496282638737	0.0
33	0.0	0.0	0.0	17.42978634848406	0.0
34	0.0	0.0	0.0	17.941169379450017	0.0
35	0.0	0.0	0.0	18.312334482570467	0.0
36	0.0	0.0	0.0	18.724359470547256	0.0
37	0.0	0.0	0.0	19.0796628171241	0.0
38	0.0	0.0	0.0	19.401846816037885	0.0
39	0.0	0.0	0.0	19.800674822570393	0.0
40	1.2689405234887234E-4	0.0	0.0	20.197980100474712	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATCA	35	1.01901605E-7	46.000004	16
ACTACGG	70	0.0	46.000004	1
ATGACGG	35	1.01901605E-7	46.000004	1
AACCTAG	35	1.01901605E-7	46.000004	18
ACGTAGG	70	0.0	46.000004	1
TCACGAC	25	3.4161712E-5	46.0	24
AACGTTC	20	6.3102413E-4	46.0	39
CGAACGT	20	6.3102413E-4	46.0	37
ACACGCG	25	3.4161712E-5	46.0	35
ATGATTC	20	6.3102413E-4	46.0	8
TCCGATT	20	6.3102413E-4	46.0	20
CTATCGG	25	3.4161712E-5	46.0	1
CGTGCCT	20	6.3102413E-4	46.0	39
GCTATCG	20	6.3102413E-4	46.0	17
ATTAACG	20	6.3102413E-4	46.0	41
ATTCGCG	20	6.3102413E-4	46.0	13
GTAATCG	20	6.3102413E-4	46.0	34
CTAACGG	85	0.0	46.0	1
CCGTACC	20	6.3102413E-4	46.0	44
TGATTCG	25	3.4161712E-5	46.0	45
>>END_MODULE