FastQCFastQC Report
Thu 26 May 2016
SRR1544803_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544803_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences555831
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT27740.4990725598248389Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCTT24270.4366435121466777Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA19240.34614837963337775No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT17180.3090867547869766Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCTTG11570.2081567958606123Illumina Single End Adapter 1 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC7770.13979069177501793No Hit
ACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC6870.12359871975474561No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC6400.11514291214415892No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC6090.10956567733717622No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT6000.107946480135149Illumina Single End Adapter 1 (95% over 21bp)
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA5850.10524781813177027No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5780.10398844253019351No Hit
GCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC5780.10398844253019351No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG5720.10290897772884204No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAA351.01828846E-746.00000425
TCGCAGG950.046.01
ATTACGG301.8596565E-646.01
GACCGAT453.092282E-1046.08
ATTCGAT453.092282E-1046.013
TCGATGG453.092282E-1046.01
GCCGCGA301.8596565E-646.025
CTAACGG750.046.01
TGATTGG551.8189894E-1246.01
ACAATTG206.308627E-446.021
ACTACGG206.308627E-446.01
TCCACGG453.092282E-1046.01
CTCGAGG206.308627E-446.01
ACGTAGG850.046.01
ACGTACT253.414862E-546.09
GTGACCG253.414862E-546.016
AGTACGG253.414862E-546.01
TAGTCGG253.414862E-546.01
TCGAAGG900.046.01
ATTCTCG253.414862E-546.013
Produced by FastQC (version 0.11.5)