FastQCFastQC Report
Thu 26 May 2016
SRR1544802_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544802_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences875141
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT17320.1979109652044642Illumina PCR Primer Index 8 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC16080.18374181988959493No Hit
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCTT13700.15654620226912005Illumina PCR Primer Index 8 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13130.15003296611631725No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT12250.13997744363479714Illumina PCR Primer Index 8 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA206.310625E-446.013
CGAACGT206.310625E-446.037
ACGTTCG206.310625E-446.040
ACGTTAT206.310625E-446.038
CGTGCAT206.310625E-446.031
GACCGTC301.8608898E-646.012
CCTACGG253.4164834E-546.01
ATTAACG206.310625E-446.020
TAACGCG206.310625E-446.033
TCGATGA405.6079443E-946.036
CGTTCGC206.310625E-446.041
ACGGGTA600.046.04
GTAATTG206.310625E-446.025
CCATAGG453.092282E-1046.01
CGAATAT600.046.014
ACGCTTA206.310625E-446.017
GCGATTC206.310625E-446.08
GTATACG206.310625E-446.036
CCAACGG501.6370905E-1146.01
ATACGTC206.310625E-446.038
Produced by FastQC (version 0.11.5)