FastQCFastQC Report
Thu 26 May 2016
SRR1544801_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544801_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences620865
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA27140.43713206574698205No Hit
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT23390.37673246196838284Illumina PCR Primer Index 8 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCTT16240.26157055076385366Illumina PCR Primer Index 8 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT14330.23080701923928715Illumina PCR Primer Index 8 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC11650.18764143573884823No Hit
TGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCTTG9900.1594549539755019Illumina PCR Primer Index 8 (96% over 26bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG9780.15752216665458674No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGT253.415328E-546.045
ATGGGCG301.8600113E-646.04
TCGCATA206.3092017E-446.022
ATCTCGG206.3092017E-446.01
GATACCA206.3092017E-446.08
CGAACGC206.3092017E-446.017
GTCGATC206.3092017E-446.041
CTCGTCG206.3092017E-446.08
CACGGTA206.3092017E-446.03
GCGGTCA253.415328E-546.021
CGCATAT206.3092017E-446.023
TAGCGCG206.3092017E-446.01
CGATTAC206.3092017E-446.011
TGACGTA206.3092017E-446.042
ACGATTC206.3092017E-446.026
AATGCGG750.046.01
GCCGATG301.8600113E-646.08
ACGATAT206.3092017E-446.027
CGGATCA206.3092017E-446.030
CCATAGG301.8600113E-646.01
Produced by FastQC (version 0.11.5)