##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544801_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	620865
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91303262383932	34.0	33.0	34.0	31.0	34.0
2	33.28008504264212	34.0	34.0	34.0	31.0	34.0
3	33.38432670548348	34.0	34.0	34.0	31.0	34.0
4	36.606212300580644	37.0	37.0	37.0	35.0	37.0
5	36.60965266201187	37.0	37.0	37.0	35.0	37.0
6	36.53384230066118	37.0	37.0	37.0	35.0	37.0
7	36.44901548645841	37.0	37.0	37.0	35.0	37.0
8	36.46843838837751	37.0	37.0	37.0	35.0	37.0
9	38.29945157159769	39.0	39.0	39.0	37.0	39.0
10	38.105175843379804	39.0	39.0	39.0	35.0	39.0
11	37.324506937901155	39.0	35.0	39.0	35.0	39.0
12	37.26052362429836	39.0	35.0	39.0	35.0	39.0
13	37.25429521715671	39.0	35.0	39.0	35.0	39.0
14	38.44156459133628	40.0	35.0	41.0	35.0	41.0
15	38.49296707013602	40.0	35.0	41.0	35.0	41.0
16	38.50067889154647	40.0	35.0	41.0	35.0	41.0
17	38.58993178871413	40.0	36.0	41.0	35.0	41.0
18	38.58185918033711	40.0	36.0	41.0	35.0	41.0
19	38.49837887463458	40.0	35.0	41.0	35.0	41.0
20	38.418759311605584	40.0	35.0	41.0	35.0	41.0
21	38.36463160268335	40.0	35.0	41.0	35.0	41.0
22	38.3488922712667	40.0	35.0	41.0	35.0	41.0
23	38.30365699467678	40.0	35.0	41.0	35.0	41.0
24	38.234877147205914	40.0	35.0	41.0	35.0	41.0
25	38.16060335177534	40.0	35.0	41.0	35.0	41.0
26	38.087577814822865	40.0	35.0	41.0	35.0	41.0
27	38.08971354481248	40.0	35.0	41.0	35.0	41.0
28	38.13731487521442	40.0	35.0	41.0	35.0	41.0
29	38.18151611058765	40.0	35.0	41.0	35.0	41.0
30	38.12320391711564	40.0	35.0	41.0	35.0	41.0
31	38.07191257358685	40.0	35.0	41.0	35.0	41.0
32	38.05402462693178	40.0	35.0	41.0	35.0	41.0
33	37.97321962101262	40.0	35.0	41.0	35.0	41.0
34	37.89510602143783	40.0	35.0	41.0	35.0	41.0
35	37.8048545174877	40.0	35.0	41.0	35.0	41.0
36	37.74897602538394	40.0	35.0	41.0	35.0	41.0
37	37.6889211020109	40.0	35.0	41.0	35.0	41.0
38	37.63141423658927	39.0	35.0	41.0	35.0	41.0
39	37.547735820186354	39.0	35.0	41.0	35.0	41.0
40	37.48635693749849	39.0	35.0	41.0	35.0	41.0
41	37.456418061897516	39.0	35.0	41.0	35.0	41.0
42	37.37996182745041	39.0	35.0	41.0	35.0	41.0
43	37.30871606548928	39.0	35.0	41.0	35.0	41.0
44	37.15211680478043	38.0	35.0	41.0	35.0	41.0
45	37.13525001409324	38.0	35.0	41.0	35.0	41.0
46	37.07172573747916	38.0	35.0	41.0	35.0	41.0
47	36.991047973391964	37.0	35.0	41.0	34.0	41.0
48	36.950615673294514	37.0	35.0	41.0	34.0	41.0
49	36.87918307522569	37.0	35.0	41.0	34.0	41.0
50	36.790828924162255	37.0	35.0	40.0	34.0	41.0
51	36.71430504215893	36.0	35.0	40.0	34.0	41.0
52	36.26588227714559	36.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	1.0
14	4.0
15	16.0
16	30.0
17	23.0
18	61.0
19	110.0
20	183.0
21	295.0
22	402.0
23	676.0
24	1006.0
25	1514.0
26	2195.0
27	2637.0
28	2698.0
29	3034.0
30	3420.0
31	4358.0
32	5919.0
33	9042.0
34	32852.0
35	149391.0
36	24119.0
37	38052.0
38	67647.0
39	268283.0
40	2892.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.315076546431186	22.167298849186214	23.030288387974842	22.487336216407755
2	26.479025230927817	24.7664145989869	26.72738840166542	22.027171768419866
3	27.94617187311251	24.604060464030024	25.487988532128565	21.961779130728903
4	26.449872355504016	26.512849009043833	23.055092491926587	23.982186143525567
5	25.58172871719295	34.41827128280705	20.00982500221465	19.99017499778535
6	88.17665676113164	3.269792950158247	5.846681645768403	2.7068686429417026
7	89.48483164617107	2.756154719625039	4.576357179096905	3.182656455106988
8	80.35321688289724	5.095310574762629	8.436294524574585	6.115178017765537
9	56.704597617839624	19.106246929686808	11.541639486844966	12.6475159656286
10	45.930274697397984	20.434393950375686	20.180554548895493	13.454776803330837
11	43.05573675436689	18.24986108091131	21.083649424593105	17.610752740128692
12	20.729788279255555	36.04696673189824	24.522400199721357	18.70084478912485
13	15.696487964372288	38.20266885715896	26.460180554548895	19.640662623919855
14	14.199060987493256	24.083818543483687	42.75019529205222	18.96692517697084
15	17.00192473404041	16.910761598737245	45.798361962745524	20.28895170447682
16	17.676950705870038	19.506494970726326	25.63262544997705	37.183928873426595
17	20.826910842131543	22.840875230525153	31.45128167959218	24.880932247751122
18	27.543346782311772	24.50935388530518	24.948096607152923	22.999202725230123
19	31.353514854275886	22.119462362993566	23.14432283990884	23.38269994282171
20	23.31263640243853	27.95390302239617	26.333905116249106	22.39955545891619
21	22.876792861572163	23.448897908563055	24.464738711314055	29.20957051855073
22	20.849298961932142	29.626891514258336	22.450130060480138	27.073679463329388
23	21.175617887946654	21.939229945318225	36.33527417393475	20.549877992800365
24	19.96633728749406	22.538555080412007	33.15680542468974	24.338302207404187
25	19.460430206244514	31.710919443035117	25.65791275075902	23.170737599961345
26	19.83200856869046	30.69604503394458	25.7028500559703	23.769096341394665
27	21.48357533441247	26.33406618185918	33.47732598874151	18.705032494986835
28	19.01862723780532	24.68137195686663	31.248500076506165	25.051500728821885
29	22.03377545843299	28.77114992792314	26.87073679463329	22.324337819010573
30	30.62710895283194	25.300830293219946	21.006176866146426	23.065883887801697
31	30.67558970146489	26.567611316469765	22.11189227931998	20.644906702745363
32	29.777648925289718	26.951752796501655	22.114308263471123	21.156290014737504
33	21.54316961014069	27.33428362043278	24.264534157989257	26.85801261143727
34	23.372391743776827	24.853228962818	28.018490332036755	23.755888961368413
35	33.70185144918783	22.327881262432253	22.66998461823424	21.300282670145684
36	24.792024030989023	29.552157071182943	23.112592914723812	22.543225983104218
37	25.175682314190684	29.42958614191491	23.007739202564164	22.38699234133024
38	23.427637248032987	28.050059191611705	23.239995812294136	25.28230774806117
39	27.846633326085378	23.572435231491546	23.9643078608071	24.616623581615972
40	18.51207589411547	22.38747553816047	28.31018015188487	30.790268415839193
41	23.600621713254892	26.018699717329852	22.17792917945125	28.202749389963998
42	24.939882261039035	23.337923703220508	24.92071545343996	26.801478582300497
43	24.591014149613848	27.36891272659918	25.70220579352999	22.33786733025698
44	20.717064096059527	31.35738042891772	22.8500559702995	25.075499504723247
45	24.037270582171647	28.048609601121015	23.898109895065755	24.016009921641583
46	25.11544377602216	24.21428168764546	25.142663864125055	25.527610672207324
47	24.96774661158223	20.952058821160797	30.154542452868178	23.925652114388797
48	22.032647999162457	20.82948789189276	28.485902732478074	28.651961376466705
49	21.511117553735517	27.699580424085752	27.268568851521668	23.520733170657067
50	20.342908683852368	29.279795124543984	24.83518961448946	25.542106577114186
51	22.933004759488778	23.171865059231877	27.475216029249516	26.41991415202983
52	27.24521433806061	21.36986301369863	27.360698380485292	24.02422426775547
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	76.0
1	148.5
2	221.0
3	713.5
4	1206.0
5	894.0
6	582.0
7	633.5
8	685.0
9	714.0
10	743.0
11	748.5
12	754.0
13	733.5
14	653.0
15	593.0
16	568.5
17	544.0
18	610.5
19	677.0
20	685.5
21	694.0
22	924.0
23	1154.0
24	1255.5
25	1357.0
26	1751.5
27	2146.0
28	3147.5
29	4149.0
30	4031.5
31	3914.0
32	4547.0
33	5180.0
34	6641.5
35	8103.0
36	8495.5
37	8888.0
38	11192.5
39	14877.5
40	16258.0
41	22946.5
42	29635.0
43	39192.5
44	48750.0
45	52984.5
46	57219.0
47	54111.5
48	51004.0
49	53472.5
50	55941.0
51	58929.0
52	61917.0
53	55363.5
54	48810.0
55	42373.0
56	35936.0
57	32959.5
58	29983.0
59	29066.0
60	28149.0
61	27923.5
62	27698.0
63	27236.0
64	21788.5
65	16803.0
66	13880.5
67	10958.0
68	8969.5
69	6981.0
70	5488.5
71	3996.0
72	3644.5
73	3293.0
74	2931.0
75	2569.0
76	1950.0
77	1331.0
78	918.0
79	505.0
80	389.5
81	274.0
82	222.5
83	171.0
84	94.0
85	17.0
86	12.5
87	8.0
88	4.0
89	2.5
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	2.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	620865.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.93601020175773
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.33156337255632	16.589974754220375
2	10.535656244274263	4.8329183820177395
3	3.583756601680771	2.465912339303003
4	1.6932909372892304	1.5534935284883877
5	0.9386553218583605	1.076450401903877
6	0.691369523540372	0.951435506706381
7	0.4748251202855393	0.7623415662043977
8	0.40255776481199523	0.7386455200419768
9	0.36931611244705004	0.762357431048314
>10	7.971323034344321	50.59903837089321
>50	0.8618181381350856	12.280063697228247
>100	0.13811639826204153	5.206246618343441
>500	0.0042280530080216795	0.6820551573764224
>1k	0.003523377506684733	1.4990667262242459
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2714	0.43713206574698205	No Hit
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT	2339	0.37673246196838284	Illumina PCR Primer Index 8 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCTT	1624	0.26157055076385366	Illumina PCR Primer Index 8 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT	1433	0.23080701923928715	Illumina PCR Primer Index 8 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1165	0.18764143573884823	No Hit
TGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCTTG	990	0.1594549539755019	Illumina PCR Primer Index 8 (96% over 26bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	978	0.15752216665458674	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2670467815064467	0.0
2	0.0	0.0	0.0	1.031303101318322	0.0
3	0.0	0.0	0.0	1.611461428813027	0.0
4	0.0	0.0	0.0	1.9672553614714954	0.0
5	0.0	0.0	0.0	3.941758675396423	0.0
6	0.0	0.0	0.0	4.230066117432936	0.0
7	0.0	0.0	0.0	5.2705499585256055	0.0
8	0.0	0.0	0.0	6.520741223937571	0.0
9	0.0	0.0	0.0	7.416588147181755	0.0
10	0.0	0.0	0.0	8.151369460349674	0.0
11	3.2213122015252915E-4	0.0	0.0	8.800302803346943	0.0
12	3.2213122015252915E-4	0.0	0.0	9.408325481384843	0.0
13	3.2213122015252915E-4	0.0	0.0	9.680687428023806	0.0
14	3.2213122015252915E-4	0.0	0.0	9.869134191813036	0.0
15	3.2213122015252915E-4	0.0	0.0	10.51049745113672	0.0
16	3.2213122015252915E-4	0.0	0.0	11.186811947846955	0.0
17	3.2213122015252915E-4	0.0	0.0	12.042392468572073	0.0
18	4.8319683022879367E-4	0.0	0.0	12.467605679173412	0.0
19	4.8319683022879367E-4	0.0	0.0	12.909086516392453	0.0
20	4.8319683022879367E-4	0.0	0.0	13.506639929775394	0.0
21	4.8319683022879367E-4	0.0	0.0	14.008842501993186	0.0
22	6.442624403050583E-4	0.0	0.0	14.596731978771553	0.0
23	6.442624403050583E-4	0.0	0.0	15.040145603311508	0.0
24	8.053280503813228E-4	0.0	0.0	15.417200196500044	0.0
25	8.053280503813228E-4	0.0	0.0	15.766068307925233	0.0
26	8.053280503813228E-4	0.0	0.0	16.075314279271662	0.0
27	9.663936604575873E-4	0.0	0.0	16.395995908933504	0.0
28	9.663936604575873E-4	0.0	0.0	16.714583685664355	0.0
29	9.663936604575873E-4	0.0	0.0	17.12739484428982	0.0
30	9.663936604575873E-4	0.0	0.0	17.53102526314094	0.0
31	9.663936604575873E-4	0.0	0.0	17.89406714825284	0.0
32	9.663936604575873E-4	0.0	0.0	18.205568038140335	0.0
33	9.663936604575873E-4	0.0	0.0	18.53172589854477	0.0
34	9.663936604575873E-4	0.0	0.0	18.993339937023347	0.0
35	9.663936604575873E-4	0.0	0.0	19.34800641041128	0.0
36	9.663936604575873E-4	0.0	0.0	19.71233682040379	0.0
37	9.663936604575873E-4	0.0	0.0	20.04831968302288	0.0
38	9.663936604575873E-4	0.0	0.0	20.364008278772356	0.0
39	9.663936604575873E-4	0.0	0.0	20.727050163884257	0.0
40	9.663936604575873E-4	0.0	0.0	21.09218590192715	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	25	3.415328E-5	46.0	45
ATGGGCG	30	1.8600113E-6	46.0	4
TCGCATA	20	6.3092017E-4	46.0	22
ATCTCGG	20	6.3092017E-4	46.0	1
GATACCA	20	6.3092017E-4	46.0	8
CGAACGC	20	6.3092017E-4	46.0	17
GTCGATC	20	6.3092017E-4	46.0	41
CTCGTCG	20	6.3092017E-4	46.0	8
CACGGTA	20	6.3092017E-4	46.0	3
GCGGTCA	25	3.415328E-5	46.0	21
CGCATAT	20	6.3092017E-4	46.0	23
TAGCGCG	20	6.3092017E-4	46.0	1
CGATTAC	20	6.3092017E-4	46.0	11
TGACGTA	20	6.3092017E-4	46.0	42
ACGATTC	20	6.3092017E-4	46.0	26
AATGCGG	75	0.0	46.0	1
GCCGATG	30	1.8600113E-6	46.0	8
ACGATAT	20	6.3092017E-4	46.0	27
CGGATCA	20	6.3092017E-4	46.0	30
CCATAGG	30	1.8600113E-6	46.0	1
>>END_MODULE