FastQCFastQC Report
Thu 26 May 2016
SRR1544800_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544800_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences994767
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT25650.2578493255204485Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT21890.22005152965468297Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCTT20980.210903658846745TruSeq Adapter, Index 27 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15320.15400591294242771No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC15310.15390538688959324No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC11640.11701232549933803No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA10520.10575340758187596No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT10040.1009281570458208No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAAC253.4168217E-546.041
CGGCGTA206.311044E-446.09
GGCGTAA206.311044E-446.010
ATTACGA501.6370905E-1146.012
TCGATGG850.046.01
TCGATAT206.311044E-446.015
CGTTCAA253.4168217E-546.09
TAACACG253.4168217E-546.028
TTACGCG253.4168217E-546.01
ACGTACT206.311044E-446.032
ACGAGTA206.311044E-446.025
CTCTACG206.311044E-446.027
GCGTAAA206.311044E-446.011
TCACGTA206.311044E-446.09
ACGGGAT1900.044.7894744
ATTGCGG2400.044.0833321
CGAATAT1150.044.014
AATGCGG1100.043.9090921
CTTGCGG3300.043.9090921
CGGTCTA1100.043.90909230
Produced by FastQC (version 0.11.5)