##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544800_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	994767
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.90868816516833	34.0	33.0	34.0	31.0	34.0
2	33.26123504297991	34.0	33.0	34.0	31.0	34.0
3	33.32979783205514	34.0	34.0	34.0	31.0	34.0
4	36.60382582051877	37.0	37.0	37.0	35.0	37.0
5	36.60037476112497	37.0	37.0	37.0	35.0	37.0
6	36.68089612944539	37.0	37.0	37.0	35.0	37.0
7	36.662421451455465	37.0	37.0	37.0	35.0	37.0
8	36.56402353515949	37.0	37.0	37.0	35.0	37.0
9	38.35409497902524	39.0	39.0	39.0	37.0	39.0
10	38.139083825659675	39.0	39.0	39.0	35.0	39.0
11	37.40494005128839	39.0	35.0	39.0	35.0	39.0
12	37.30411845185858	39.0	35.0	39.0	35.0	39.0
13	37.286928496823876	39.0	35.0	39.0	35.0	39.0
14	38.54324278951754	41.0	35.0	41.0	35.0	41.0
15	38.594258756070516	41.0	35.0	41.0	35.0	41.0
16	38.58702389604802	41.0	35.0	41.0	35.0	41.0
17	38.65037943558642	41.0	36.0	41.0	35.0	41.0
18	38.63023803564051	40.0	36.0	41.0	35.0	41.0
19	38.55029670264494	40.0	35.0	41.0	35.0	41.0
20	38.47558976122047	40.0	35.0	41.0	35.0	41.0
21	38.40231129500677	40.0	35.0	41.0	35.0	41.0
22	38.367191513188516	40.0	35.0	41.0	35.0	41.0
23	38.33209183658083	40.0	35.0	41.0	35.0	41.0
24	38.27753936348914	40.0	35.0	41.0	35.0	41.0
25	38.23306362193358	40.0	35.0	41.0	35.0	41.0
26	38.16150113544177	40.0	35.0	41.0	35.0	41.0
27	38.152052691735854	40.0	35.0	41.0	35.0	41.0
28	38.18391743996333	40.0	35.0	41.0	35.0	41.0
29	38.1742840283202	40.0	35.0	41.0	35.0	41.0
30	38.061613423042786	40.0	35.0	41.0	35.0	41.0
31	38.003476190907016	40.0	35.0	41.0	35.0	41.0
32	37.95457830828727	40.0	35.0	41.0	35.0	41.0
33	37.83924175208868	40.0	35.0	41.0	35.0	41.0
34	37.78012137515619	40.0	35.0	41.0	35.0	41.0
35	37.70335364964861	40.0	35.0	41.0	35.0	41.0
36	37.620089930606866	40.0	35.0	41.0	35.0	41.0
37	37.56596569850025	40.0	35.0	41.0	35.0	41.0
38	37.51474968510214	39.0	35.0	41.0	35.0	41.0
39	37.409239550568124	39.0	35.0	41.0	34.0	41.0
40	37.34004746840215	39.0	35.0	41.0	34.0	41.0
41	37.29503089668234	39.0	35.0	41.0	34.0	41.0
42	37.213883251052756	39.0	35.0	41.0	34.0	41.0
43	37.185208194481724	39.0	35.0	41.0	34.0	41.0
44	37.13635353806469	39.0	35.0	41.0	34.0	41.0
45	37.09357166049939	38.0	35.0	41.0	34.0	41.0
46	37.0499212378376	38.0	35.0	41.0	34.0	41.0
47	36.96288578129351	38.0	35.0	41.0	34.0	41.0
48	36.91620650865982	37.0	35.0	41.0	34.0	41.0
49	36.83986802939784	37.0	35.0	41.0	34.0	41.0
50	36.740807646413685	37.0	35.0	41.0	34.0	41.0
51	36.618082425331764	37.0	35.0	40.0	34.0	41.0
52	36.31108993362265	36.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	4.0
13	3.0
14	6.0
15	12.0
16	16.0
17	37.0
18	79.0
19	140.0
20	287.0
21	442.0
22	754.0
23	1175.0
24	2011.0
25	3050.0
26	4260.0
27	4975.0
28	5319.0
29	5820.0
30	6756.0
31	8335.0
32	10972.0
33	16086.0
34	54653.0
35	215983.0
36	39885.0
37	59483.0
38	105727.0
39	445085.0
40	3409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.51558706712225	22.748342074073626	24.248492360522615	19.487578498281508
2	27.573391558023136	23.675996489630236	26.856942379471775	21.893669572874856
3	27.30036279852468	24.824104539052865	25.29295804947289	22.582574612949564
4	26.52540745722365	26.84055663285976	22.58046356584004	24.053572344076553
5	24.957502611164223	35.8170305207149	19.680689045776546	19.54477782234433
6	88.7794830347207	3.066748293821568	5.819453198588212	2.3343154728695263
7	89.80313983073424	2.779243782714947	4.393692191236742	3.0239241953140783
8	81.03314645540112	5.306770329132349	8.418252716465263	5.241830499001273
9	53.67397591596826	19.001635558879617	13.73929774510011	13.585090780052012
10	43.66188263181227	20.504500048755137	20.820855537025253	15.012761782407338
11	40.88585568278803	18.481614287566835	23.245644457445813	17.386885572199322
12	22.00505243941546	34.8401183392694	24.831945571173954	18.32288365014119
13	16.212439696934055	36.138311785573904	27.932470618747907	19.716777898744127
14	16.032799640518835	23.814320338330482	41.76143760297638	18.391442418174307
15	18.63411230971675	18.689401638775713	42.593290690181725	20.083195361325817
16	19.37599457963523	20.98069196103208	25.2034898624502	34.43982359688248
17	21.697040613530604	23.73842316844045	30.301367053792493	24.26316916423645
18	26.940379003324395	25.338295299301244	25.28019124076291	22.441134456611447
19	29.09254126845784	23.710275873646793	23.93153371593549	23.265649141959877
20	23.606734039227277	28.205398852193532	26.206036187368497	21.981830921210697
21	22.744019453801744	23.933041606728008	25.951805799750094	27.371133139720154
22	20.789491408540894	27.919703810037927	24.631798199980498	26.659006581440682
23	21.675226460065524	21.95368362641704	35.59527004816203	20.775819865355405
24	20.645538100881915	22.513010584388102	32.153660103320675	24.687791211409305
25	19.27908746470279	30.713322818308207	26.253685536412046	23.75390418057696
26	19.59353295796905	29.09414968530319	28.031488780789875	23.280828575937885
27	20.3149079131093	26.17648152783516	33.51287286369572	19.995737695359818
28	18.6269749599655	25.14739632496856	31.427258845538702	24.798369869527235
29	22.197559830593498	27.469146041233778	28.82182460817458	21.51146951999815
30	29.163110557547643	24.93166741558576	24.20999088228701	21.695231144579587
31	28.99814730484626	27.187874145402898	24.25804233554189	19.555936214208955
32	28.689431796591563	27.074078653594256	23.917560594591496	20.31892895522268
33	19.93743258471582	26.294499113862845	28.009875679430458	25.758192621990876
34	21.646777587113363	25.040939235016847	29.966313719695165	23.34596945817463
35	28.880732875135585	25.550505796834837	24.51699744764352	21.05176388038606
36	22.57593989346249	28.639269296227155	26.36738050216784	22.41741030814251
37	23.154266275419268	28.93833430340974	25.800212512075692	22.107186909095294
38	21.488750632057556	29.42015567464542	25.224700859598276	23.866392833698747
39	26.536565849088277	23.79381302355225	25.963768400037395	23.70585272732208
40	18.882612712323592	22.427563439478792	29.53837431277877	29.151449535418845
41	22.549702593672688	26.065701817611565	23.980288851560214	27.404306737155537
42	23.277209638035842	24.05578391723891	26.240918727702066	26.426087717023183
43	23.304050094142646	27.302071741422868	27.054375547238703	22.339502617195787
44	20.742445215814357	30.18907945277638	24.76278364682383	24.305691684585433
45	22.768949914904695	27.52121853660204	26.302340145983933	23.407491402509333
46	24.05397444828789	24.94755053193361	26.53415322382025	24.46432179595825
47	23.438855530993692	21.762684126031523	31.154632190251586	23.643828152723202
48	21.206272423592658	21.979317769889832	29.74254272608561	27.0718670804319
49	20.791300877491913	26.78798150722732	29.40939938699213	23.011318228288637
50	19.59504084876157	27.689901253258302	27.910153835018654	24.804904062961477
51	21.754843093910434	23.112145859281622	28.69697125055415	26.436039796253795
52	23.93806790936973	21.849237057522014	29.726358031579252	24.486337001529
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	110.0
1	279.5
2	449.0
3	1773.5
4	3098.0
5	2131.5
6	1165.0
7	1214.5
8	1264.0
9	1286.0
10	1308.0
11	1275.0
12	1242.0
13	1230.5
14	1241.5
15	1264.0
16	1234.5
17	1205.0
18	1201.5
19	1198.0
20	1248.5
21	1299.0
22	1584.0
23	1869.0
24	2170.0
25	2471.0
26	3393.5
27	4316.0
28	6775.0
29	9234.0
30	9240.5
31	9247.0
32	11161.5
33	13076.0
34	15016.0
35	16956.0
36	18640.5
37	20325.0
38	22183.0
39	26695.0
40	29349.0
41	40504.0
42	51659.0
43	65814.0
44	79969.0
45	88454.5
46	96940.0
47	97343.5
48	97747.0
49	97244.5
50	96742.0
51	88379.5
52	80017.0
53	71845.5
54	63674.0
55	58531.5
56	53389.0
57	49513.0
58	45637.0
59	44568.0
60	43499.0
61	42249.5
62	41000.0
63	37813.0
64	27820.5
65	21015.0
66	18233.0
67	15451.0
68	12920.0
69	10389.0
70	8017.5
71	5646.0
72	5199.0
73	4752.0
74	4108.5
75	3465.0
76	2662.0
77	1859.0
78	1391.5
79	924.0
80	618.0
81	312.0
82	273.5
83	235.0
84	168.0
85	101.0
86	54.0
87	7.0
88	5.0
89	2.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	994767.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.304540336327907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.22714435380165	15.387661102633457
2	11.292058114748176	4.811442151716227
3	3.9558266774550903	2.5283120704009194
4	1.852554610875171	1.578712977305613
5	1.0339647814277861	1.1014072196135367
6	0.6670047566645745	0.8526137845729805
7	0.5081048800338854	0.7577458638236876
8	0.3684990371255667	0.6280562080271704
9	0.29176365175993707	0.5594301438834517
>10	5.332492820856585	31.350679123559882
>50	2.2401370650112873	30.827948588975122
>100	0.2195656100851835	7.245059887953492
>500	0.007098026188098604	1.0467461221804621
>1k	0.0037856139669859225	1.3241847553539798
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	2565	0.2578493255204485	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	2189	0.22005152965468297	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCTT	2098	0.210903658846745	TruSeq Adapter, Index 27 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1532	0.15400591294242771	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1531	0.15390538688959324	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1164	0.11701232549933803	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1052	0.10575340758187596	No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT	1004	0.1009281570458208	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2173373262281519	0.0
2	0.0	0.0	0.0	0.8342657124733731	0.0
3	0.0	0.0	0.0	1.35489014010316	0.0
4	0.0	0.0	0.0	1.6745629881168154	0.0
5	0.0	0.0	0.0	3.458900425928886	0.0
6	0.0	0.0	0.0	3.7120250269661135	0.0
7	0.0	0.0	0.0	4.515931871483473	0.0
8	0.0	0.0	0.0	5.54763075172377	0.0
9	0.0	0.0	0.0	6.227890551254716	0.0
10	0.0	0.0	0.0	6.873468862557765	0.0
11	0.0	0.0	0.0	7.464763105330193	0.0
12	0.0	0.0	0.0	7.98046175637109	0.0
13	0.0	0.0	0.0	8.231274258193125	0.0
14	0.0	0.0	0.0	8.423379545159822	0.0
15	1.0052605283448285E-4	0.0	0.0	9.058201568809581	0.0
16	1.0052605283448285E-4	0.0	0.0	9.730218232008099	0.0
17	1.0052605283448285E-4	0.0	0.0	10.556341334201878	0.0
18	1.0052605283448285E-4	0.0	0.0	10.977545495578362	0.0
19	1.0052605283448285E-4	0.0	0.0	11.443182172307687	0.0
20	1.0052605283448285E-4	0.0	0.0	12.032264841917756	0.0
21	1.0052605283448285E-4	0.0	0.0	12.56113240587997	0.0
22	1.0052605283448285E-4	0.0	0.0	13.133226172561011	0.0
23	2.010521056689657E-4	0.0	0.0	13.572826601606206	0.0
24	2.010521056689657E-4	0.0	0.0	13.962767160551165	0.0
25	2.010521056689657E-4	0.0	0.0	14.33089356603104	0.0
26	2.010521056689657E-4	0.0	0.0	14.678713708838352	0.0
27	2.010521056689657E-4	0.0	0.0	15.037893295615959	0.0
28	2.010521056689657E-4	0.0	0.0	15.391644475540504	0.0
29	2.010521056689657E-4	0.0	0.0	15.84541907803536	0.0
30	2.010521056689657E-4	0.0	0.0	16.259083785449256	0.0
31	2.010521056689657E-4	0.0	0.0	16.64822013597154	0.0
32	2.010521056689657E-4	0.0	0.0	17.019864953300623	0.0
33	2.010521056689657E-4	0.0	0.0	17.398647120380954	0.0
34	2.010521056689657E-4	0.0	0.0	17.89343635243228	0.0
35	2.010521056689657E-4	0.0	0.0	18.260658023436644	0.0
36	2.010521056689657E-4	0.0	0.0	18.657534880027182	0.0
37	2.010521056689657E-4	0.0	0.0	19.026666546035404	0.0
38	2.010521056689657E-4	0.0	0.0	19.426358132105307	0.0
39	2.010521056689657E-4	0.0	0.0	19.83992231346637	0.0
40	2.010521056689657E-4	0.0	0.0	20.274898544081175	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCAAC	25	3.4168217E-5	46.0	41
CGGCGTA	20	6.311044E-4	46.0	9
GGCGTAA	20	6.311044E-4	46.0	10
ATTACGA	50	1.6370905E-11	46.0	12
TCGATGG	85	0.0	46.0	1
TCGATAT	20	6.311044E-4	46.0	15
CGTTCAA	25	3.4168217E-5	46.0	9
TAACACG	25	3.4168217E-5	46.0	28
TTACGCG	25	3.4168217E-5	46.0	1
ACGTACT	20	6.311044E-4	46.0	32
ACGAGTA	20	6.311044E-4	46.0	25
CTCTACG	20	6.311044E-4	46.0	27
GCGTAAA	20	6.311044E-4	46.0	11
TCACGTA	20	6.311044E-4	46.0	9
ACGGGAT	190	0.0	44.789474	4
ATTGCGG	240	0.0	44.083332	1
CGAATAT	115	0.0	44.0	14
AATGCGG	110	0.0	43.909092	1
CTTGCGG	330	0.0	43.909092	1
CGGTCTA	110	0.0	43.909092	30
>>END_MODULE