FastQCFastQC Report
Thu 26 May 2016
SRR1544799_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544799_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences636952
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA33000.5180924151270426No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT27790.4362966126175913Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCTT21220.3331491226968437TruSeq Adapter, Index 27 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT18210.2858928145291953Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCTTG10940.1717554854996923Illumina Single End Adapter 1 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC9790.15370074982102264No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG9170.14396689232469637No Hit
ACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC7360.11555030834348584TruSeq Adapter, Index 27 (95% over 22bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC7120.11178236350619826No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6710.10534545774249864No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA206.309324E-446.07
CAAACGT206.309324E-446.039
ATTACGG551.8189894E-1246.01
ATTACGA453.092282E-1046.012
TACGGGT900.046.03
CCGTAGG453.092282E-1046.01
CGTAGAT206.309324E-446.038
TGTTCGG253.415429E-546.01
TCCGTCA206.309324E-446.028
ATGCGCG206.309324E-446.01
TTACGAT453.092282E-1046.013
CGGTCTA800.046.030
CACACGG650.046.01
GCTACGG501.6370905E-1146.01
TAAGCCG206.309324E-446.017
AGTACGG253.415429E-546.01
GCACTAT206.309324E-446.08
CTTACGT453.092282E-1046.022
CCCGAAA206.309324E-446.021
TATGCGG1600.044.56251
Produced by FastQC (version 0.11.5)