##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544799_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	636952
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.897378452379456	34.0	33.0	34.0	31.0	34.0
2	33.27068758713372	34.0	34.0	34.0	31.0	34.0
3	33.36987716499831	34.0	34.0	34.0	31.0	34.0
4	36.591097916326504	37.0	37.0	37.0	35.0	37.0
5	36.598354350092315	37.0	37.0	37.0	35.0	37.0
6	36.52288084502443	37.0	37.0	37.0	35.0	37.0
7	36.42981574749746	37.0	37.0	37.0	35.0	37.0
8	36.45844270839875	37.0	37.0	37.0	35.0	37.0
9	38.286343397932654	39.0	39.0	39.0	37.0	39.0
10	38.13645612228237	39.0	39.0	39.0	35.0	39.0
11	37.45834536982379	39.0	35.0	39.0	35.0	39.0
12	37.398110061668696	39.0	35.0	39.0	35.0	39.0
13	37.38780473253872	39.0	35.0	39.0	35.0	39.0
14	38.61157983647119	41.0	35.0	41.0	35.0	41.0
15	38.658603788040544	41.0	35.0	41.0	35.0	41.0
16	38.66071069719539	41.0	35.0	41.0	35.0	41.0
17	38.738554867556736	41.0	36.0	41.0	35.0	41.0
18	38.729185244728015	41.0	36.0	41.0	35.0	41.0
19	38.65155145128675	41.0	35.0	41.0	35.0	41.0
20	38.5825085720745	41.0	35.0	41.0	35.0	41.0
21	38.5215338047451	40.0	35.0	41.0	35.0	41.0
22	38.490426280159255	40.0	35.0	41.0	35.0	41.0
23	38.46600999761363	40.0	35.0	41.0	35.0	41.0
24	38.398829111141815	40.0	35.0	41.0	35.0	41.0
25	38.34238372750223	40.0	35.0	41.0	35.0	41.0
26	38.25310698451375	40.0	35.0	41.0	35.0	41.0
27	38.263029239251935	40.0	35.0	41.0	35.0	41.0
28	38.30293962496389	40.0	35.0	41.0	35.0	41.0
29	38.34067088257828	40.0	35.0	41.0	35.0	41.0
30	38.24010600484809	40.0	35.0	41.0	35.0	41.0
31	38.20689471106143	40.0	35.0	41.0	35.0	41.0
32	38.19829280699331	40.0	35.0	41.0	35.0	41.0
33	38.06148029992841	40.0	35.0	41.0	35.0	41.0
34	38.043846318089905	40.0	35.0	41.0	35.0	41.0
35	37.94893178763863	40.0	35.0	41.0	35.0	41.0
36	37.89778193647245	40.0	35.0	41.0	35.0	41.0
37	37.85074542508698	40.0	35.0	41.0	35.0	41.0
38	37.77823446664741	40.0	35.0	41.0	35.0	41.0
39	37.743048141775205	40.0	35.0	41.0	35.0	41.0
40	37.676919139903795	40.0	35.0	41.0	35.0	41.0
41	37.653415014004196	40.0	35.0	41.0	35.0	41.0
42	37.592163930720055	39.0	35.0	41.0	35.0	41.0
43	37.50339743026162	39.0	35.0	41.0	35.0	41.0
44	37.354403471533175	39.0	35.0	41.0	35.0	41.0
45	37.31420421005036	39.0	35.0	41.0	35.0	41.0
46	37.26113113704015	39.0	35.0	41.0	35.0	41.0
47	37.181517602582296	38.0	35.0	41.0	34.0	41.0
48	37.107651753978324	38.0	35.0	41.0	34.0	41.0
49	37.03937345357264	38.0	35.0	41.0	34.0	41.0
50	36.94007397731697	37.0	35.0	41.0	34.0	41.0
51	36.80571691430438	37.0	35.0	40.0	34.0	41.0
52	36.301495246109596	36.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	2.0
13	5.0
14	4.0
15	6.0
16	17.0
17	35.0
18	52.0
19	95.0
20	165.0
21	235.0
22	451.0
23	649.0
24	1018.0
25	1640.0
26	2187.0
27	2737.0
28	2797.0
29	2931.0
30	3685.0
31	4644.0
32	6155.0
33	9380.0
34	31497.0
35	136256.0
36	24374.0
37	38205.0
38	70177.0
39	296074.0
40	1474.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.263812657782694	22.6510003893543	23.139106243484594	21.946080709378414
2	27.00093570630126	24.177646039261987	26.807514537987164	22.01390371644959
3	27.624844572275464	24.818981650108643	24.844258280058778	22.71191549755712
4	26.375299865609968	26.38409173689697	23.04726258807571	24.193345809417348
5	24.806892827089012	34.91032291287255	19.86617515919567	20.416609100842763
6	87.17736972330725	3.2727740865873725	6.552299074341551	2.997557115763825
7	88.48861452668334	2.8697609866991547	4.971018224293196	3.67060626232432
8	78.69619688767756	5.4751378439819645	9.417978120800312	6.41068714754016
9	53.01184390660521	18.557756314447555	14.11676233059948	14.313637448347757
10	42.31873045378616	20.352711036310428	22.11626621786257	15.212292292040845
11	39.36827264848843	18.310641932202113	23.604133435486503	18.71695198382296
12	21.56222132907974	33.88010399527751	25.32310126979741	19.23457340584534
13	16.084885517276028	35.226045290696945	28.315634459111514	20.37343473291551
14	15.727715746241474	23.652331729863473	40.79271279468469	19.827239729210362
15	18.24376091134026	18.721504917168012	42.14964392921288	20.885090242278853
16	18.976626182192692	21.771499265250757	25.00219796782175	34.2496765847348
17	20.94773232519876	24.045924967658475	30.273395797485524	24.73294690965724
18	26.239339856064507	25.269879048970722	25.43833758273779	23.05244351222698
19	28.260371268164636	23.655785679297654	24.127877767869478	23.95596528466823
20	22.954162951054396	28.459758349137772	26.578611889121945	22.00746681068589
21	22.43622753362891	23.812940378552856	25.802258254939147	27.948573832879088
22	20.346588125949836	28.062711161908588	24.723998040668686	26.866702671472893
23	21.09232720832967	21.998517941697333	35.7447656966302	21.164389153342793
24	21.09311219683744	22.876920081890002	31.278495082831988	24.75147263844057
25	19.74701390371645	30.276849746919705	25.794408369861465	24.18172797950238
26	19.744501940491592	28.61864002311006	27.53771084791319	24.09914718848516
27	21.729580878935934	25.53190821286376	32.57827905399465	20.160231854205655
28	19.308205327874	25.066881020861853	30.64312538464437	24.981788266619777
29	23.263448423115086	26.662291664050038	28.12802220575491	21.94623770707997
30	29.227006116630456	25.125755158944475	23.767725040505407	21.87951368391967
31	28.98852660797046	26.845350984061593	23.70210000125598	20.464022406711965
32	28.86810937087881	26.713786910159637	23.743076401361485	20.67502731760007
33	19.658467200040192	25.96318089903164	28.314849470603747	26.06350243032442
34	22.21423278363205	24.255516899232596	30.289880556148656	23.2403697609867
35	28.69164395433251	25.36486265841068	24.921187153820068	21.022306233436744
36	23.09546088245268	27.99002122608925	25.93602029666286	22.97849759479521
37	23.453886635099664	28.835139853552544	26.124888531631896	21.586084979715896
38	21.11509187505495	29.198746530350796	24.808776799507655	24.8773847950866
39	27.21445258041422	23.079290119192656	25.736005224883506	23.970252075509617
40	19.09060651352064	21.657518933922805	29.605684572777857	29.646189979778697
41	22.366049561034426	26.17245883520265	23.84229894874339	27.619192655019532
42	22.951493990127986	23.710420879438324	26.212493249098834	27.12559188133486
43	23.93131664552431	26.676264459488312	26.77470201836245	22.617716876624925
44	21.198771649983044	28.878157223778246	25.145065876235574	24.77800525000314
45	22.488036775141612	26.73294062974918	26.473109433677887	24.305913161431317
46	24.21956442557681	23.801950539444103	26.618489305316572	25.359995729662515
47	22.809411070221934	21.319973875582463	31.70333086323616	24.167284190959446
48	20.99216267473844	21.175535990153104	29.63143219583265	28.200869139275802
49	20.71035180044964	25.67587510518846	29.655609841871915	23.95816325248998
50	19.41794672126	26.63214810535174	28.63276981624989	25.31713535713837
51	21.958012534696493	22.18675818586016	28.47844107562265	27.376788203820695
52	23.94984237430764	21.4421180873912	30.351894648262352	24.25614489003881
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	61.0
1	157.0
2	253.0
3	866.0
4	1479.0
5	1039.5
6	600.0
7	635.5
8	671.0
9	686.0
10	701.0
11	714.5
12	728.0
13	703.5
14	716.5
15	754.0
16	733.5
17	713.0
18	725.0
19	737.0
20	733.5
21	730.0
22	897.5
23	1065.0
24	1282.5
25	1500.0
26	2009.5
27	2519.0
28	3836.5
29	5154.0
30	5200.5
31	5247.0
32	6452.5
33	7658.0
34	8799.0
35	9940.0
36	10874.0
37	11808.0
38	13375.0
39	16054.5
40	17167.0
41	24220.0
42	31273.0
43	40147.5
44	49022.0
45	54847.5
46	60673.0
47	63485.5
48	66298.0
49	66821.5
50	67345.0
51	60757.0
52	54169.0
53	47966.5
54	41764.0
55	38145.0
56	34526.0
57	31580.0
58	28634.0
59	28016.0
60	27398.0
61	26305.5
62	25213.0
63	24841.5
64	18995.0
65	13520.0
66	11758.0
67	9996.0
68	8262.5
69	6529.0
70	5068.5
71	3608.0
72	3319.5
73	3031.0
74	2591.0
75	2151.0
76	1694.0
77	1237.0
78	925.5
79	614.0
80	405.0
81	196.0
82	160.5
83	125.0
84	86.0
85	47.0
86	26.0
87	5.0
88	3.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	636952.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.28606004762327
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.72181705839716	18.388482329109053
2	10.334208224109164	5.226228193989332
3	3.455618596687248	2.6213693801255227
4	1.5887677991016984	1.6069471187926325
5	0.9220549231722605	1.165756807727022
6	0.6864869137692756	1.041512959408649
7	0.5128781124962697	0.9078066724784651
8	0.433824608669221	0.8775772083957273
9	0.4101063532823753	0.9332976487508952
>10	8.415576985913333	53.37228352284703
>50	0.4126841357621446	6.836130488886767
>100	0.09600204970901852	4.091621451301278
>500	0.006857289264929894	1.1787627696895233
>1k	0.003116949665877225	1.7522234484980932
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	3300	0.5180924151270426	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	2779	0.4362966126175913	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCTT	2122	0.3331491226968437	TruSeq Adapter, Index 27 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	1821	0.2858928145291953	Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCTTG	1094	0.1717554854996923	Illumina Single End Adapter 1 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	979	0.15370074982102264	No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	917	0.14396689232469637	No Hit
ACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	736	0.11555030834348584	TruSeq Adapter, Index 27 (95% over 22bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	712	0.11178236350619826	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	671	0.10534545774249864	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.34037101696831157	0.0
2	0.0	0.0	0.0	1.2457767618282067	0.0
3	0.0	0.0	0.0	1.9378226302766928	0.0
4	0.0	0.0	0.0	2.3614024290684386	0.0
5	0.0	0.0	0.0	4.654981851065701	0.0
6	0.0	0.0	0.0	4.990171943882742	0.0
7	0.0	0.0	0.0	6.039544581067333	0.0
8	0.0	0.0	0.0	7.338857559125334	0.0
9	0.0	0.0	0.0	8.174085331390748	0.0
10	0.0	0.0	0.0	8.988746404752634	0.0
11	1.5699770155364927E-4	0.0	0.0	9.699945992790665	0.0
12	1.5699770155364927E-4	0.0	0.0	10.35713837149424	0.0
13	1.5699770155364927E-4	0.0	0.0	10.67411673093106	0.0
14	1.5699770155364927E-4	0.0	0.0	10.909299287858426	0.0
15	1.5699770155364927E-4	0.0	0.0	11.661004282897299	0.0
16	1.5699770155364927E-4	0.0	0.0	12.442695838932918	0.0
17	1.5699770155364927E-4	0.0	0.0	13.363173363141964	0.0
18	1.5699770155364927E-4	0.0	0.0	13.849395244853616	0.0
19	1.5699770155364927E-4	0.0	0.0	14.36450470365114	0.0
20	1.5699770155364927E-4	0.0	0.0	15.02279606626559	0.0
21	3.1399540310729854E-4	0.0	0.0	15.608868486165363	0.0
22	3.1399540310729854E-4	0.0	0.0	16.258210979791254	0.0
23	3.1399540310729854E-4	0.0	0.0	16.72732011203356	0.0
24	3.1399540310729854E-4	0.0	0.0	17.171937602833495	0.0
25	3.1399540310729854E-4	0.0	0.0	17.579032642962108	0.0
26	3.1399540310729854E-4	0.0	0.0	17.965560984187192	0.0
27	3.1399540310729854E-4	0.0	0.0	18.339215513884877	0.0
28	3.1399540310729854E-4	0.0	0.0	18.724330875795978	0.0
29	3.1399540310729854E-4	0.0	0.0	19.210081764402968	0.0
30	3.1399540310729854E-4	0.0	0.0	19.660351172458835	0.0
31	3.1399540310729854E-4	0.0	0.0	20.053630414850726	0.0
32	3.1399540310729854E-4	0.0	0.0	20.430895891684145	0.0
33	3.1399540310729854E-4	0.0	0.0	20.80784737311446	0.0
34	3.1399540310729854E-4	0.0	0.0	21.325782790539947	0.0
35	3.1399540310729854E-4	0.0	0.0	21.68954646503975	0.0
36	3.1399540310729854E-4	0.0	0.0	22.08784963388136	0.0
37	3.1399540310729854E-4	0.0	0.0	22.466685087730315	0.0
38	3.1399540310729854E-4	0.0	0.0	22.87723407729311	0.0
39	3.1399540310729854E-4	0.0	0.0	23.283073135809293	0.0
40	4.709931046609478E-4	0.0	0.0	23.737110488702445	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	20	6.309324E-4	46.0	7
CAAACGT	20	6.309324E-4	46.0	39
ATTACGG	55	1.8189894E-12	46.0	1
ATTACGA	45	3.092282E-10	46.0	12
TACGGGT	90	0.0	46.0	3
CCGTAGG	45	3.092282E-10	46.0	1
CGTAGAT	20	6.309324E-4	46.0	38
TGTTCGG	25	3.415429E-5	46.0	1
TCCGTCA	20	6.309324E-4	46.0	28
ATGCGCG	20	6.309324E-4	46.0	1
TTACGAT	45	3.092282E-10	46.0	13
CGGTCTA	80	0.0	46.0	30
CACACGG	65	0.0	46.0	1
GCTACGG	50	1.6370905E-11	46.0	1
TAAGCCG	20	6.309324E-4	46.0	17
AGTACGG	25	3.415429E-5	46.0	1
GCACTAT	20	6.309324E-4	46.0	8
CTTACGT	45	3.092282E-10	46.0	22
CCCGAAA	20	6.309324E-4	46.0	21
TATGCGG	160	0.0	44.5625	1
>>END_MODULE