FastQCFastQC Report
Thu 26 May 2016
SRR1544798_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544798_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1470523
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT71630.4871056080047711TruSeq Adapter, Index 16 (100% over 24bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCTT64110.4359673395111808TruSeq Adapter, Index 16 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT56940.38720917659907395TruSeq Adapter, Index 16 (100% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23550.160147104125539No Hit
TCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT21260.1445744133209749TruSeq Adapter, Index 16 (100% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC20120.1368220694269998TruSeq Adapter, Index 16 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC20040.13627804529408927TruSeq Adapter, Index 16 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC19480.13246987636371552No Hit
GCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC19220.13070179793175626TruSeq Adapter, Index 16 (100% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC16780.11410906187798492TruSeq Adapter, Index 16 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAACG206.312032E-446.00000438
TACTTCG206.312032E-446.00000445
GAGTACG206.312032E-446.0000049
TTGTCGT206.312032E-446.00000426
ATCGTAC206.312032E-446.0000049
AATGCGC206.312032E-446.00000436
ATCGGGT206.312032E-446.0000043
TATCGGC206.312032E-446.00000425
TATCGAC206.312032E-446.00000414
ACCGCGT253.4176242E-546.024
CCTACGG253.4176242E-546.01
ATTCGAC301.8617611E-646.018
TCGATGG1150.046.01
CTAACGG1200.046.01
ACGTAGT253.4176242E-546.022
AGTACGG1200.044.0833321
TACGCGG2250.043.9555551
GCGATAC1000.043.78
CACGGGC1700.043.2941173
ACGGGAT5050.042.8118864
Produced by FastQC (version 0.11.5)