FastQCFastQC Report
Thu 26 May 2016
SRR1544797_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544797_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences859179
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT62080.7225502485512332TruSeq Adapter, Index 16 (100% over 24bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCTT51290.5969652424000121TruSeq Adapter, Index 16 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT35900.41784075262547155TruSeq Adapter, Index 16 (100% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA31500.3666290726379486No Hit
TGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCTTG26170.30459310574397186TruSeq Adapter, Index 16 (100% over 26bp)
TCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT14140.16457571705081248TruSeq Adapter, Index 16 (100% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC13240.15410060068972822TruSeq Adapter, Index 16 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC12760.1485138719638166TruSeq Adapter, Index 16 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC12640.14711718978233873TruSeq Adapter, Index 16 (100% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC10900.1268652981509092TruSeq Adapter, Index 16 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC10070.1172049130623537No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9710.11301486651792002No Hit
ACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT9270.1078936985191677TruSeq Adapter, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGG750.046.0000041
CGGGTAC405.6079443E-946.05
GGTAATC253.4164295E-546.07
TACTTCG253.4164295E-546.045
CGACGGT405.6079443E-946.027
CGACCTT253.4164295E-546.024
CACGCGA206.310561E-446.018
ACCTCGC206.310561E-446.021
CTAAACG253.4164295E-546.044
GCGATAA206.310561E-446.08
ACGTCGA253.4164295E-546.028
CGTCGAT253.4164295E-546.029
CCGGTCA206.310561E-446.029
CGGTCGT206.310561E-446.045
CGCACAA253.4164295E-546.029
CACGTCG253.4164295E-546.027
TAAGCGT206.310561E-446.019
CCGCACA253.4164295E-546.028
GAATCGG351.0191616E-745.9999961
CTAACGG650.045.9999961
Produced by FastQC (version 0.11.5)