##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544797_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	859179
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89424438912031	34.0	33.0	34.0	31.0	34.0
2	33.2433963120607	34.0	33.0	34.0	31.0	34.0
3	33.33767235931046	34.0	34.0	34.0	31.0	34.0
4	36.57341950862393	37.0	37.0	37.0	35.0	37.0
5	36.57367905872932	37.0	37.0	37.0	35.0	37.0
6	36.50938628621044	37.0	37.0	37.0	35.0	37.0
7	36.40427431303605	37.0	37.0	37.0	35.0	37.0
8	36.43231852733831	37.0	37.0	37.0	35.0	37.0
9	38.27312702009709	39.0	39.0	39.0	37.0	39.0
10	38.133021174865775	39.0	39.0	39.0	35.0	39.0
11	37.52912838884563	39.0	35.0	39.0	35.0	39.0
12	37.48435308591108	39.0	35.0	39.0	35.0	39.0
13	37.47239981424127	39.0	35.0	39.0	35.0	39.0
14	38.721132616137034	41.0	35.0	41.0	35.0	41.0
15	38.782195561111244	41.0	35.0	41.0	35.0	41.0
16	38.78355267063092	41.0	35.0	41.0	35.0	41.0
17	38.85172938351612	41.0	36.0	41.0	35.0	41.0
18	38.84663731306282	41.0	36.0	41.0	35.0	41.0
19	38.79248212537783	41.0	36.0	41.0	35.0	41.0
20	38.71278860400452	41.0	35.0	41.0	35.0	41.0
21	38.64602835963169	41.0	35.0	41.0	35.0	41.0
22	38.63064157759908	41.0	35.0	41.0	35.0	41.0
23	38.60766033620468	41.0	35.0	41.0	35.0	41.0
24	38.526527068282626	40.0	35.0	41.0	35.0	41.0
25	38.46533027459935	40.0	35.0	41.0	35.0	41.0
26	38.40946182343842	40.0	35.0	41.0	35.0	41.0
27	38.391478376450074	40.0	35.0	41.0	35.0	41.0
28	38.44758891918913	40.0	36.0	41.0	35.0	41.0
29	38.47733359404734	40.0	36.0	41.0	35.0	41.0
30	38.42824021536839	40.0	35.0	41.0	35.0	41.0
31	38.38030724680189	40.0	35.0	41.0	35.0	41.0
32	38.36084564450481	40.0	35.0	41.0	35.0	41.0
33	38.27743811243059	40.0	35.0	41.0	35.0	41.0
34	38.23525714664814	40.0	35.0	41.0	35.0	41.0
35	38.15322185481722	40.0	35.0	41.0	35.0	41.0
36	38.111882390049104	40.0	35.0	41.0	35.0	41.0
37	38.04828446691551	40.0	35.0	41.0	35.0	41.0
38	38.00058893431986	40.0	35.0	41.0	35.0	41.0
39	37.96048087767508	40.0	35.0	41.0	35.0	41.0
40	37.90255115639465	40.0	35.0	41.0	35.0	41.0
41	37.882363279363204	40.0	35.0	41.0	35.0	41.0
42	37.812948174943756	40.0	35.0	41.0	35.0	41.0
43	37.746546412330844	40.0	35.0	41.0	35.0	41.0
44	37.62178544866669	40.0	35.0	41.0	35.0	41.0
45	37.60526619016526	39.0	35.0	41.0	35.0	41.0
46	37.56060262180524	39.0	35.0	41.0	35.0	41.0
47	37.48191354770077	39.0	35.0	41.0	35.0	41.0
48	37.448503746018	39.0	35.0	41.0	35.0	41.0
49	37.404197495516065	39.0	35.0	41.0	35.0	41.0
50	37.320853978041825	39.0	35.0	41.0	35.0	41.0
51	37.25479905817065	39.0	35.0	41.0	34.0	41.0
52	36.838526081293885	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	3.0
14	8.0
15	7.0
16	26.0
17	39.0
18	68.0
19	117.0
20	207.0
21	328.0
22	494.0
23	769.0
24	1302.0
25	2156.0
26	3054.0
27	3752.0
28	3858.0
29	4178.0
30	4942.0
31	6270.0
32	8104.0
33	12134.0
34	39431.0
35	165460.0
36	31326.0
37	48685.0
38	86754.0
39	431416.0
40	4289.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.48065886154108	22.054775547353927	23.537353682992716	21.92721190811228
2	27.190608709011745	24.14246623811802	26.527883013900478	22.139042038969762
3	27.675373816166367	24.329621650436057	25.648438800296564	22.346565733101016
4	26.755658599663168	25.808591690439357	23.11078366673301	24.324966043164462
5	24.489774540578853	34.2820297051022	20.94871964980522	20.279476104513726
6	85.30585593921639	3.110643998514861	7.976102767874912	3.607397294393834
7	86.639571032346	2.621688844815807	6.138301797413578	4.60043832542462
8	75.5770334237685	5.559377033190988	11.711994822964714	7.151594720075794
9	48.863740850276834	22.243560422217023	14.381636422677929	14.511062304828215
10	39.89180368700818	20.727927474949922	25.283788360749043	14.096480477292857
11	36.46830287984227	19.79296514463226	24.184715874107724	19.554016101417748
12	20.34535294740677	32.806085809825426	28.378137733813325	18.47042350895448
13	16.244694062587655	35.07173708854616	29.17424657725573	19.509322271610454
14	14.888515664372617	25.858173907881827	40.21001444402156	19.043295983723997
15	16.8081389326322	19.45205830216986	43.31914537017315	20.420657395024786
16	17.5047341706443	22.171980460416282	26.989952035606084	33.33333333333333
17	20.11175785255459	23.9882492472465	30.635990870354142	25.26400202984477
18	24.173891587201272	26.868324295635716	27.208300016643793	21.74948410051922
19	28.02221655790004	22.649645766481722	25.268308466570993	24.059829209047244
20	22.173609922961337	29.05750722492054	27.01160060941899	21.757282242699137
21	21.47049683476901	23.8475335174626	26.627513009512572	28.054456638255825
22	19.181916690235678	28.73627032318062	24.436235056955535	27.645577929628168
23	19.63304503485304	23.284670598327008	35.44558235245508	21.636702014364875
24	20.598967153526797	22.6201990504889	32.31422090158163	24.46661289440268
25	17.768241542216465	30.58303333763977	27.49403791293782	24.15468720720595
26	18.20330804174683	27.86206366775724	27.642900955447004	26.291727335048925
27	19.853022478435808	26.075008816556274	33.8781557742915	20.19381293071642
28	16.924296334058443	25.645412655570027	30.52448907619949	26.90580193417204
29	19.311691742931334	25.555908605773652	27.402206059505644	27.73019359178937
30	24.05272940795806	24.834173088494946	25.091395390250458	26.02170211329653
31	24.544012365292915	26.732380563305203	23.224496874341668	25.499110197060215
32	22.14846964369474	24.16702456647567	25.366541780001604	28.31796400982799
33	16.46420594544327	24.850351323763732	27.653958022717035	31.03148470807597
34	17.94329237562836	23.368122358670313	28.454024132340294	30.234561133361034
35	24.074028811225602	21.663704536540116	27.879173024480348	26.38309362775394
36	20.001187179854256	25.80184105989555	25.369102364000984	28.827869396249213
37	19.749667997006444	26.755309429117798	27.407560007867975	26.08746256600778
38	18.8613781295865	25.222799905491172	26.842485675278375	29.073336289643954
39	24.428669695139195	21.370634058793335	26.376226607028336	27.82446963903913
40	16.251793863676838	20.581741406621905	31.12890329023405	32.03756143946721
41	19.888405093699916	25.01283201754233	24.730120265974843	30.36864262278291
42	20.725832451677707	21.56139756674686	28.996751550026246	28.71601843154919
43	21.81314952995825	25.558352799591237	26.91930319525966	25.70919447519085
44	18.15896338248491	26.720857935308008	25.328482190556333	29.79169649165075
45	20.62957777133752	24.060061989410823	27.09900963594315	28.211350603308503
46	21.89997660557346	21.846087951404773	27.89174316411365	28.362192278908122
47	19.787727586451716	20.189739274353773	33.36266365914437	26.65986948005014
48	18.969504608469247	19.302962479297097	29.272829061231707	32.454703851001945
49	19.153983046606122	23.268143192512852	30.801846879404643	26.776026881476383
50	18.252424698462136	24.289001476991405	28.769674305354297	28.688899519192155
51	20.140389837274885	20.83128195637929	28.433190289799914	30.595137916545912
52	21.329548324621527	19.30726891602332	32.70959834912166	26.65358441023349
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	96.0
1	185.5
2	275.0
3	1178.5
4	2082.0
5	1486.5
6	891.0
7	948.5
8	1006.0
9	1038.5
10	1071.0
11	1080.5
12	1090.0
13	1055.5
14	1050.5
15	1080.0
16	1008.0
17	936.0
18	1096.0
19	1256.0
20	1404.5
21	1553.0
22	1836.5
23	2120.0
24	2355.0
25	2590.0
26	3276.5
27	3963.0
28	4711.0
29	5459.0
30	6389.0
31	7319.0
32	8364.0
33	9409.0
34	10823.0
35	12237.0
36	14210.5
37	16184.0
38	17650.0
39	21742.5
40	24369.0
41	31530.5
42	38692.0
43	49530.0
44	60368.0
45	66488.0
46	72608.0
47	73388.5
48	74169.0
49	85051.5
50	95934.0
51	98593.5
52	101253.0
53	89265.5
54	77278.0
55	65876.0
56	54474.0
57	46232.0
58	37990.0
59	34585.5
60	31181.0
61	30942.0
62	30703.0
63	28338.5
64	20449.5
65	14925.0
66	12262.0
67	9599.0
68	7947.0
69	6295.0
70	5205.5
71	4116.0
72	3599.0
73	3082.0
74	2810.5
75	2539.0
76	2136.5
77	1734.0
78	1203.0
79	672.0
80	464.5
81	257.0
82	213.0
83	169.0
84	102.5
85	36.0
86	19.0
87	2.0
88	1.5
89	1.0
90	1.0
91	1.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	859179.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.86678679908282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.84097165711057	17.36720552038155
2	12.47527657156151	6.20440085529226
3	4.745009509506868	3.5397941949758356
4	2.2042740035845254	2.1925284677558845
5	1.274516334128911	1.5846562976366119
6	0.7512517053654264	1.1208729593861666
7	0.5509989632552457	0.9591101622048703
8	0.42467592250660735	0.8448260498940499
9	0.3509250637403638	0.785374086823756
>10	6.60382628800739	45.256624552175104
>50	0.6381376390028224	10.041081087326997
>100	0.12602865677037878	5.336915225994848
>500	0.008934867457601482	1.4842889844990603
>1k	0.004232305637811228	1.9565999597130919
>5k	9.405123639580507E-4	1.3257215959399549
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	6208	0.7225502485512332	TruSeq Adapter, Index 16 (100% over 24bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCTT	5129	0.5969652424000121	TruSeq Adapter, Index 16 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	3590	0.41784075262547155	TruSeq Adapter, Index 16 (100% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	3150	0.3666290726379486	No Hit
TGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCTTG	2617	0.30459310574397186	TruSeq Adapter, Index 16 (100% over 26bp)
TCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	1414	0.16457571705081248	TruSeq Adapter, Index 16 (100% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	1324	0.15410060068972822	TruSeq Adapter, Index 16 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	1276	0.1485138719638166	TruSeq Adapter, Index 16 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	1264	0.14711718978233873	TruSeq Adapter, Index 16 (100% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	1090	0.1268652981509092	TruSeq Adapter, Index 16 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1007	0.1172049130623537	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	971	0.11301486651792002	No Hit
ACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	927	0.1078936985191677	TruSeq Adapter, Index 16 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6098845525786827	0.0
2	0.0	0.0	0.0	2.052890026408932	0.0
3	0.0	0.0	0.0	3.105406440334319	0.0
4	0.0	0.0	0.0	3.7673174041730535	0.0
5	0.0	0.0	0.0	6.942907124126637	0.0
6	0.0	0.0	0.0	7.440125980732769	0.0
7	0.0	0.0	0.0	9.053875851248693	0.0
8	0.0	0.0	0.0	10.959648687875285	0.0
9	0.0	0.0	0.0	12.278233057372212	0.0
10	0.0	0.0	0.0	13.39755743564496	0.0
11	0.0	0.0	0.0	14.470674911747144	0.0
12	0.0	0.0	0.0	15.39807188024847	0.0
13	0.0	0.0	0.0	15.814748731056042	0.0
14	0.0	0.0	0.0	16.113638717892314	0.0
15	1.1639018178982494E-4	0.0	0.0	17.084798394746613	0.0
16	1.1639018178982494E-4	0.0	0.0	18.11124340795108	0.0
17	1.1639018178982494E-4	0.0	0.0	19.361739521100958	0.0
18	1.1639018178982494E-4	0.0	0.0	19.99757908421877	0.0
19	1.1639018178982494E-4	0.0	0.0	20.680323890597883	0.0
20	1.1639018178982494E-4	0.0	0.0	21.544288210023755	0.0
21	1.1639018178982494E-4	0.0	0.0	22.290000104751165	0.0
22	1.1639018178982494E-4	0.0	0.0	23.146631842724275	0.0
23	1.1639018178982494E-4	0.0	0.0	23.779678041479134	0.0
24	1.1639018178982494E-4	0.0	0.0	24.31961209480213	0.0
25	1.1639018178982494E-4	0.0	0.0	24.83300918667705	0.0
26	1.1639018178982494E-4	0.0	0.0	25.285883384021258	0.0
27	1.1639018178982494E-4	0.0	0.0	25.772277953720938	0.0
28	1.1639018178982494E-4	0.0	0.0	26.249826869604586	0.0
29	1.1639018178982494E-4	0.0	0.0	26.842136504733006	0.0
30	1.1639018178982494E-4	0.0	0.0	27.40604693550471	0.0
31	1.1639018178982494E-4	0.0	0.0	27.90291662156547	0.0
32	1.1639018178982494E-4	0.0	0.0	28.371852663996677	0.0
33	1.1639018178982494E-4	0.0	0.0	28.843931241336207	0.0
34	1.1639018178982494E-4	0.0	0.0	29.444155408826333	0.0
35	1.1639018178982494E-4	0.0	0.0	29.934740025070447	0.0
36	1.1639018178982494E-4	0.0	0.0	30.427885225313933	0.0
37	1.1639018178982494E-4	0.0	0.0	30.871331817933168	0.0
38	2.3278036357964987E-4	0.0	0.0	31.338987568364683	0.0
39	2.3278036357964987E-4	0.0	0.0	31.82445101661004	0.0
40	2.3278036357964987E-4	0.0	0.0	32.31026363540077	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCACGG	75	0.0	46.000004	1
CGGGTAC	40	5.6079443E-9	46.0	5
GGTAATC	25	3.4164295E-5	46.0	7
TACTTCG	25	3.4164295E-5	46.0	45
CGACGGT	40	5.6079443E-9	46.0	27
CGACCTT	25	3.4164295E-5	46.0	24
CACGCGA	20	6.310561E-4	46.0	18
ACCTCGC	20	6.310561E-4	46.0	21
CTAAACG	25	3.4164295E-5	46.0	44
GCGATAA	20	6.310561E-4	46.0	8
ACGTCGA	25	3.4164295E-5	46.0	28
CGTCGAT	25	3.4164295E-5	46.0	29
CCGGTCA	20	6.310561E-4	46.0	29
CGGTCGT	20	6.310561E-4	46.0	45
CGCACAA	25	3.4164295E-5	46.0	29
CACGTCG	25	3.4164295E-5	46.0	27
TAAGCGT	20	6.310561E-4	46.0	19
CCGCACA	25	3.4164295E-5	46.0	28
GAATCGG	35	1.0191616E-7	45.999996	1
CTAACGG	65	0.0	45.999996	1
>>END_MODULE