FastQCFastQC Report
Thu 26 May 2016
SRR1544796_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544796_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1484948
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT70360.4738213055271969TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCTT70180.4726091418689409TruSeq Adapter, Index 13 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC68380.4604875052863804No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT64100.43166494719007TruSeq Adapter, Index 19 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC56770.3823029493288654No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG41890.2820974202463655No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC31000.20876151892187472No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28900.19461960957555416No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA28020.18869347613519125No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC27490.1851243275858818No Hit
ACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC23520.1583893846787901TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT22720.15300199064209655TruSeq Adapter, Index 19 (95% over 22bp)
GGGGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT20590.1386580540194No Hit
CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC19350.130307593262525TruSeq Adapter, Index 13 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC17670.11899406578546859TruSeq Adapter, Index 19 (95% over 21bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA17480.11771455970175386No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC17090.11508820510886576No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA16240.10936409894487889No Hit
AGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC15740.10599697767194542TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT15190.1022931442717186TruSeq Adapter, Index 13 (95% over 22bp)
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC14930.10054224120979321No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG405.6134013E-946.00000439
AACCGTA206.3120516E-446.00000418
CGAACCG206.3120516E-446.00000416
TATATCG351.0198346E-746.0000041
ATTCGGC405.6134013E-946.00000416
CGCGATA206.3120516E-446.00000410
CCGCTAA206.3120516E-446.00000419
CCGTACA206.3120516E-446.00000420
ATGACGG800.046.0000041
CCGAATA206.3120516E-446.00000411
CACGTAT206.3120516E-446.00000430
CGACGGT1050.046.027
ACGTAAG253.417641E-546.012
TACGGGT1450.045.9999963
TGCCGTA301.8617739E-645.99999644
ATCACGG1400.044.3571431
TAATCGT2400.044.0833320
ATTGCGG2900.043.620691
CGTTTTT24000.043.41251
GGCGATA1750.043.371437
Produced by FastQC (version 0.11.5)