FastQCFastQC Report
Thu 26 May 2016
SRR1544795_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544795_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1007187
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA90200.8955635845180686No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT78840.7827742018115801TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCTT64260.6380145891477949TruSeq Adapter, Index 13 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT53040.5266152164394496TruSeq Adapter, Index 19 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC49880.4952407050527856No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG35340.3508782381027555No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC35230.3497860873899286No Hit
TGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCTTG33050.32814164599026796TruSeq Adapter, Index 19 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT19850.1970835604510384TruSeq Adapter, Index 19 (95% over 22bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC19650.19509783188226218No Hit
ACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC19270.1913249476015874TruSeq Adapter, Index 13 (95% over 21bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC18310.18179345047146162No Hit
GGGGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT18030.1790134304751749No Hit
CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC16730.16610619477812957TruSeq Adapter, Index 13 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC15260.15151108979762445TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12920.12827806554294288No Hit
ACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT12530.12440589483382926TruSeq Adapter, Index 13 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC12230.12142730198066497TruSeq Adapter, Index 13 (95% over 21bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCACAATCCCTCG11410.11328581484868251No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA10640.10564075985889414No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC10490.10415146343231198No Hit
GGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC10120.10047786558007599TruSeq Adapter, Index 19 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAT351.01939804E-746.0000048
TTAACCG351.01939804E-746.00000436
ATACCCC351.01939804E-746.00000426
ATCTCGT206.3110807E-446.018
CTCACGT551.8189894E-1246.044
GCGACTA301.8611718E-646.017
CGACGGT600.046.027
CGTTGAC253.416853E-546.013
GTAGCGT253.416853E-546.010
TTAACGA206.3110807E-446.035
GTATCCG253.416853E-546.027
CACGTTC551.8189894E-1246.046
TAGCTAA253.416853E-546.014
ACGCGCA253.416853E-546.016
CGTAGGG4750.044.0631562
TAGTAGG2300.044.01
TCGGCGT12550.043.80084
CGTTTTT12400.043.4032251
AGGGTAT3500.043.371435
TATTAGT1600.043.12530
Produced by FastQC (version 0.11.5)