##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544795_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1007187
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83361282462939	34.0	33.0	34.0	31.0	34.0
2	33.2014740063166	34.0	33.0	34.0	31.0	34.0
3	33.31017675962855	34.0	34.0	34.0	31.0	34.0
4	36.51472268804105	37.0	37.0	37.0	35.0	37.0
5	36.524976990370206	37.0	37.0	37.0	35.0	37.0
6	36.47618068938539	37.0	37.0	37.0	35.0	37.0
7	36.39846523038919	37.0	37.0	37.0	35.0	37.0
8	36.44939618958545	37.0	37.0	37.0	35.0	37.0
9	38.28298419260773	39.0	39.0	39.0	37.0	39.0
10	38.15956024055116	39.0	39.0	39.0	35.0	39.0
11	37.56744477440634	39.0	35.0	39.0	35.0	39.0
12	37.51606206196069	39.0	35.0	39.0	35.0	39.0
13	37.46506557372166	39.0	35.0	39.0	35.0	39.0
14	38.76231325463891	41.0	35.0	41.0	35.0	41.0
15	38.813269035442275	41.0	35.0	41.0	35.0	41.0
16	38.81982888976923	41.0	36.0	41.0	35.0	41.0
17	38.91158940693238	41.0	36.0	41.0	35.0	41.0
18	38.90563222122605	41.0	36.0	41.0	35.0	41.0
19	38.83867047529406	41.0	36.0	41.0	35.0	41.0
20	38.79449695041735	41.0	35.0	41.0	35.0	41.0
21	38.74426000335588	41.0	35.0	41.0	35.0	41.0
22	38.706707890391755	41.0	35.0	41.0	35.0	41.0
23	38.6975090027969	41.0	35.0	41.0	35.0	41.0
24	38.61569301430618	41.0	35.0	41.0	35.0	41.0
25	38.547077156476405	40.0	35.0	41.0	35.0	41.0
26	38.4580549590096	40.0	35.0	41.0	35.0	41.0
27	38.458346861109206	40.0	36.0	41.0	35.0	41.0
28	38.49375538008334	40.0	36.0	41.0	35.0	41.0
29	38.49055637135904	40.0	36.0	41.0	35.0	41.0
30	38.434844770633454	40.0	35.0	41.0	35.0	41.0
31	38.44075429885414	40.0	35.0	41.0	35.0	41.0
32	38.34293730955622	40.0	35.0	41.0	35.0	41.0
33	38.30403291543676	40.0	35.0	41.0	35.0	41.0
34	38.24507067704408	40.0	35.0	41.0	35.0	41.0
35	38.133831155485524	40.0	35.0	41.0	35.0	41.0
36	38.12246583802214	40.0	35.0	41.0	35.0	41.0
37	38.08231142776863	40.0	35.0	41.0	35.0	41.0
38	38.05413393937769	40.0	35.0	41.0	35.0	41.0
39	38.0394554337973	40.0	35.0	41.0	35.0	41.0
40	37.9781480499649	40.0	35.0	41.0	35.0	41.0
41	37.98235382307357	40.0	35.0	41.0	35.0	41.0
42	37.93005767548628	40.0	35.0	41.0	35.0	41.0
43	37.861739676941816	40.0	35.0	41.0	35.0	41.0
44	37.71169405482795	40.0	35.0	41.0	35.0	41.0
45	37.71250224635544	40.0	35.0	41.0	35.0	41.0
46	37.67553493045482	40.0	35.0	41.0	35.0	41.0
47	37.58265247665032	39.0	35.0	41.0	35.0	41.0
48	37.53506449149959	39.0	35.0	41.0	35.0	41.0
49	37.51117021963151	39.0	35.0	41.0	35.0	41.0
50	37.43891352847088	39.0	35.0	41.0	35.0	41.0
51	37.359566793455436	39.0	35.0	41.0	34.0	41.0
52	36.92300238188142	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	3.0
14	10.0
15	15.0
16	13.0
17	36.0
18	72.0
19	125.0
20	197.0
21	325.0
22	517.0
23	903.0
24	1518.0
25	2464.0
26	3533.0
27	4043.0
28	4232.0
29	4678.0
30	5752.0
31	7164.0
32	9544.0
33	14623.0
34	49175.0
35	183955.0
36	33901.0
37	54779.0
38	104730.0
39	517932.0
40	2945.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.76690425908992	21.176703035285406	24.115978462787943	21.94041424283673
2	27.86900545777497	23.97707674940205	26.20139060571671	21.952527187106266
3	29.32613308154295	23.804715509632274	25.145181579984648	21.723969828840126
4	27.577699076735502	25.54709304230495	24.767793865488734	22.107414015470813
5	24.144175808464567	32.61588960143449	21.991050321340524	21.248884268760417
6	83.50266633703572	2.441155416025028	10.346638707608419	3.7095395393308292
7	86.94323894172582	1.9834449809221129	6.275994428045636	4.797321649306435
8	76.22288611747372	3.6176995930249296	12.224045782957882	7.9353685065434725
9	48.43201907888009	17.832537552609395	16.260634817566153	17.474808550944363
10	40.505685637324554	19.563596432440054	25.7720760891473	14.158641841088098
11	36.97525881489733	18.810906018445433	23.410846248015513	20.80298891864172
12	22.150702898270133	28.603725028222165	29.156353288912584	20.089218784595115
13	16.872338503177662	31.888815085977086	30.09242573623369	21.146420674611566
14	15.821689517438173	24.711498460563927	38.33677360807874	21.130038413919163
15	17.22301816842354	19.523981147492968	41.701789240726896	21.551211443356596
16	18.830664017704756	22.837963555923576	26.502923488885383	31.828448937486286
17	19.295820934940583	24.321004937514086	29.68257135963828	26.70060276790705
18	22.669673059719795	27.99013490047032	27.83912024281489	21.501071796994996
19	24.90590128744712	23.222202033981773	25.01700280087015	26.854893877700963
20	20.98567594696913	29.781957074505527	27.681155535168745	21.551211443356596
21	20.219681151563712	23.559577317816853	27.315384332800164	28.90535719781927
22	18.23514401992877	28.33743882714928	25.81893928337042	27.608477869551535
23	19.36522214841931	23.736009301152617	35.020110466080276	21.878658084347794
24	23.51618914858909	22.461270846426732	29.166182645328025	24.85635735965615
25	17.616390997898108	29.80767225947118	26.945542386865597	25.63039435576512
26	16.33450392032463	30.68248498044554	27.122470802343557	25.86054029688628
27	21.640668515379964	26.581558340208915	30.254560473874264	21.52321267053685
28	16.994857955871154	28.156638240962206	27.783718415746034	27.06478538742061
29	20.171527233770888	30.526903147081924	26.863035364832943	22.438534254314245
30	23.82099848389624	25.606863472225115	23.067613064902545	27.504524978976097
31	24.777523935475738	29.519344471284874	21.556473624063855	24.146657969175536
32	21.29217315155974	29.984600674949142	21.07443801399343	27.648788159497688
33	15.698276486888732	26.270891105623882	28.53938742259382	29.491444984893572
34	17.86748637541986	25.796202691257932	25.843165171909487	30.493145761412727
35	23.63563072200098	25.16196098639081	24.42912785808395	26.77328043352426
36	19.491812344678795	26.720658626451694	24.736320067673628	29.051208961195883
37	19.449317753306982	26.56557322523027	27.531034455369262	26.45407456609349
38	17.03328180367697	25.36142742112438	24.886937579615303	32.71835319558335
39	24.476983916591458	22.900017573697834	25.926267912512767	26.696730597197938
40	16.729564619082655	20.05863856463596	31.08598502562086	32.12581179066053
41	18.442652655365883	26.3075277977178	23.568016664234147	31.681802882682163
42	19.633494078060977	22.648822909747643	26.319243596273584	31.3984394159178
43	21.955704352816312	27.075011889549806	26.085821203013936	24.883462554619946
44	19.05902280311402	27.842893127095564	24.687471144881734	28.41061292490868
45	20.114139678133256	24.605857700705034	25.475904673114325	29.80409794804738
46	22.48470244353829	22.742549298193882	26.177760435748276	28.594987822519553
47	18.191557277844133	22.925137040092853	30.946388307235896	27.936917374827118
48	18.487132975306473	20.845185650728215	27.53381447536555	33.13386689859976
49	19.584645155269083	23.05738656277335	30.229540293907686	27.128427988049886
50	18.592475875880048	23.400520459457876	28.85869257645303	29.148311088209038
51	19.820648995668133	20.312017530011804	26.938393764018002	32.92893971030206
52	20.054170675356215	19.938402699796562	32.42367107597695	27.583755548870272
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	133.0
1	201.5
2	270.0
3	1281.0
4	2292.0
5	1600.0
6	908.0
7	982.5
8	1057.0
9	1071.5
10	1086.0
11	1125.0
12	1164.0
13	1084.5
14	986.5
15	968.0
16	911.0
17	854.0
18	832.0
19	810.0
20	927.5
21	1045.0
22	1101.0
23	1157.0
24	1261.5
25	1366.0
26	1916.5
27	2467.0
28	3656.0
29	4845.0
30	5541.5
31	6238.0
32	6886.5
33	7535.0
34	9407.5
35	11280.0
36	12459.5
37	13639.0
38	15715.0
39	20783.0
40	23775.0
41	32158.5
42	40542.0
43	54040.0
44	67538.0
45	80278.5
46	93019.0
47	108728.0
48	124437.0
49	133196.0
50	141955.0
51	125351.0
52	108747.0
53	92734.5
54	76722.0
55	67548.0
56	58374.0
57	50969.5
58	43565.0
59	42372.0
60	41179.0
61	36869.0
62	32559.0
63	33164.0
64	24859.5
65	15950.0
66	12908.0
67	9866.0
68	8089.0
69	6312.0
70	5155.5
71	3999.0
72	3456.5
73	2914.0
74	2248.5
75	1583.0
76	1458.5
77	1334.0
78	1013.5
79	693.0
80	510.5
81	328.0
82	209.0
83	90.0
84	48.5
85	7.0
86	12.5
87	18.0
88	9.5
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1007187.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.13278792821782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.31738262825766	17.170062894069122
2	12.914647272547228	6.4916218213733226
3	5.253303854406779	3.9609051508588458
4	2.544284806213243	2.5577988185417686
5	1.468146912350361	1.8449312497784711
6	0.9501265494766076	1.432759744377896
7	0.6557911765330454	1.153730239550104
8	0.48429523109998485	0.9737351470306527
9	0.3767169482993572	0.8521152453515828
>10	6.427671383698542	41.98078717404791
>50	0.45622039219574523	7.457740383206776
>100	0.1335762532422551	6.0571040072977045
>500	0.009116480191607906	1.6186014820086678
>1k	0.0071346366716931444	3.5956303478348635
>5k	0.0015854748159318099	2.852476294672341
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	9020	0.8955635845180686	No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT	7884	0.7827742018115801	TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCTT	6426	0.6380145891477949	TruSeq Adapter, Index 13 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT	5304	0.5266152164394496	TruSeq Adapter, Index 19 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	4988	0.4952407050527856	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	3534	0.3508782381027555	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3523	0.3497860873899286	No Hit
TGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCTTG	3305	0.32814164599026796	TruSeq Adapter, Index 19 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT	1985	0.1970835604510384	TruSeq Adapter, Index 19 (95% over 22bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1965	0.19509783188226218	No Hit
ACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	1927	0.1913249476015874	TruSeq Adapter, Index 13 (95% over 21bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1831	0.18179345047146162	No Hit
GGGGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT	1803	0.1790134304751749	No Hit
CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	1673	0.16610619477812957	TruSeq Adapter, Index 13 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	1526	0.15151108979762445	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1292	0.12827806554294288	No Hit
ACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT	1253	0.12440589483382926	TruSeq Adapter, Index 13 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	1223	0.12142730198066497	TruSeq Adapter, Index 13 (95% over 21bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCACAATCCCTCG	1141	0.11328581484868251	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	1064	0.10564075985889414	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1049	0.10415146343231198	No Hit
GGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	1012	0.10047786558007599	TruSeq Adapter, Index 19 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6558861462667807	0.0
2	0.0	0.0	0.0	2.322905279754405	0.0
3	0.0	0.0	0.0	3.502428049607471	0.0
4	0.0	0.0	0.0	4.209744565805556	0.0
5	0.0	0.0	0.0	7.956814375086255	0.0
6	0.0	0.0	0.0	8.528704202893802	0.0
7	0.0	0.0	0.0	10.455555919605793	0.0
8	0.0	0.0	0.0	12.682749082345184	0.0
9	0.0	0.0	0.0	14.283147022350368	0.0
10	0.0	0.0	0.0	15.647044689814305	0.0
11	0.0	0.0	0.0	16.945711173793942	0.0
12	0.0	0.0	0.0	18.070527121577225	0.0
13	9.928642843881027E-5	0.0	0.0	18.59585161444697	0.0
14	9.928642843881027E-5	0.0	0.0	18.93203546114078	0.0
15	9.928642843881027E-5	0.0	0.0	19.997279551860778	0.0
16	9.928642843881027E-5	0.0	0.0	21.11951405250465	0.0
17	9.928642843881027E-5	0.0	0.0	22.55569223987204	0.0
18	9.928642843881027E-5	0.0	0.0	23.34084931596615	0.0
19	9.928642843881027E-5	0.0	0.0	24.07616460498398	0.0
20	9.928642843881027E-5	0.0	0.0	25.126615017866595	0.0
21	9.928642843881027E-5	0.0	0.0	26.041142310216475	0.0
22	9.928642843881027E-5	0.0	0.0	27.05217601100888	0.0
23	9.928642843881027E-5	0.0	0.0	27.776172647184683	0.0
24	9.928642843881027E-5	0.0	0.0	28.40058499563636	0.0
25	9.928642843881027E-5	0.0	0.0	29.02092660052205	0.0
26	9.928642843881027E-5	0.0	0.0	29.642757501834318	0.0
27	9.928642843881027E-5	0.0	0.0	30.23897250460937	0.0
28	9.928642843881027E-5	0.0	0.0	30.856534089498773	0.0
29	9.928642843881027E-5	0.0	0.0	31.53277395359551	0.0
30	9.928642843881027E-5	0.0	0.0	32.50280235944268	0.0
31	9.928642843881027E-5	0.0	0.0	33.09008158365825	0.0
32	1.9857285687762053E-4	0.0	0.0	33.68490657643516	0.0
33	1.9857285687762053E-4	0.0	0.0	34.37216723408861	0.0
34	1.9857285687762053E-4	0.0	0.0	35.23387414650904	0.0
35	1.9857285687762053E-4	0.0	0.0	35.78699883934165	0.0
36	1.9857285687762053E-4	0.0	0.0	36.37735594283882	0.0
37	1.9857285687762053E-4	0.0	0.0	36.96503231276814	0.0
38	1.9857285687762053E-4	0.0	0.0	37.74175004244495	0.0
39	1.9857285687762053E-4	0.0	0.0	38.305498383120515	0.0
40	1.9857285687762053E-4	0.0	0.0	38.86219738737692	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAT	35	1.01939804E-7	46.000004	8
TTAACCG	35	1.01939804E-7	46.000004	36
ATACCCC	35	1.01939804E-7	46.000004	26
ATCTCGT	20	6.3110807E-4	46.0	18
CTCACGT	55	1.8189894E-12	46.0	44
GCGACTA	30	1.8611718E-6	46.0	17
CGACGGT	60	0.0	46.0	27
CGTTGAC	25	3.416853E-5	46.0	13
GTAGCGT	25	3.416853E-5	46.0	10
TTAACGA	20	6.3110807E-4	46.0	35
GTATCCG	25	3.416853E-5	46.0	27
CACGTTC	55	1.8189894E-12	46.0	46
TAGCTAA	25	3.416853E-5	46.0	14
ACGCGCA	25	3.416853E-5	46.0	16
CGTAGGG	475	0.0	44.063156	2
TAGTAGG	230	0.0	44.0	1
TCGGCGT	1255	0.0	43.8008	4
CGTTTTT	1240	0.0	43.403225	1
AGGGTAT	350	0.0	43.37143	5
TATTAGT	160	0.0	43.125	30
>>END_MODULE