FastQCFastQC Report
Thu 26 May 2016
SRR1544794_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544794_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1099614
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT58780.5345512152446221RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTT53580.48726189371906864TruSeq Adapter, Index 10 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT41870.38076997928363954Illumina PCR Primer Index 2 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC19880.1807907138323084TruSeq Adapter, Index 10 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT18560.16878650144505253Illumina PCR Primer Index 2 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16060.14605125071161335No Hit
GCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC14540.13222821826568232Illumina PCR Primer Index 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC14120.12840869614246456TruSeq Adapter, Index 10 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT12240.11131178759091827TruSeq Adapter, Index 10 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC12220.11112990558505076TruSeq Adapter, Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC206.311333E-446.0000047
ATCTAGT206.311333E-446.00000434
GCGAAAC206.311333E-446.0000048
CTTCACG405.6115823E-946.00000415
CGACCCA206.311333E-446.00000428
TACGGAC206.311333E-446.00000438
AAGCCGT206.311333E-446.00000446
TCGCTAG206.311333E-446.00000410
GCGATAG453.092282E-1046.0000048
ACGTCGT206.311333E-446.00000413
CGTGTAT405.6115823E-946.00000421
AACTCGC206.311333E-446.00000436
TTTTGCG206.311333E-446.00000418
CGCGTTG206.311333E-446.00000421
CACGTCG206.311333E-446.00000412
ATCGGGT206.311333E-446.0000043
CACCGCA206.311333E-446.00000418
CAATCGG351.0195072E-746.0000041
TCGGTCG206.311333E-446.0000041
TCGGTAC453.092282E-1046.00000435
Produced by FastQC (version 0.11.5)