##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544794_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1099614
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.928388507239816	34.0	33.0	34.0	31.0	34.0
2	33.22578559385384	34.0	33.0	34.0	31.0	34.0
3	33.275326614612034	34.0	34.0	34.0	31.0	34.0
4	36.55943722069745	37.0	37.0	37.0	35.0	37.0
5	36.54668092621593	37.0	37.0	37.0	35.0	37.0
6	36.67628094949682	37.0	37.0	37.0	35.0	37.0
7	36.66243427239013	37.0	37.0	37.0	35.0	37.0
8	36.584847955737196	37.0	37.0	37.0	35.0	37.0
9	38.356477818579975	39.0	39.0	39.0	37.0	39.0
10	38.16471234451362	39.0	39.0	39.0	35.0	39.0
11	37.37756703716031	39.0	35.0	39.0	35.0	39.0
12	37.26892982446567	39.0	35.0	39.0	35.0	39.0
13	37.22889122910403	39.0	35.0	39.0	35.0	39.0
14	38.48837137395486	41.0	35.0	41.0	35.0	41.0
15	38.56065219249664	41.0	35.0	41.0	35.0	41.0
16	38.56502372650767	41.0	35.0	41.0	35.0	41.0
17	38.64450616307177	41.0	36.0	41.0	35.0	41.0
18	38.61832697655723	40.0	36.0	41.0	35.0	41.0
19	38.54107077574494	40.0	35.0	41.0	35.0	41.0
20	38.46250138685029	40.0	35.0	41.0	35.0	41.0
21	38.4081741411077	40.0	35.0	41.0	35.0	41.0
22	38.37898753562614	40.0	35.0	41.0	35.0	41.0
23	38.36776723468417	40.0	35.0	41.0	35.0	41.0
24	38.341546215308284	40.0	35.0	41.0	35.0	41.0
25	38.31952667026793	40.0	35.0	41.0	35.0	41.0
26	38.26825049517376	40.0	35.0	41.0	35.0	41.0
27	38.283245757147505	40.0	35.0	41.0	35.0	41.0
28	38.3203687839551	40.0	35.0	41.0	35.0	41.0
29	38.30835820569764	40.0	36.0	41.0	35.0	41.0
30	38.23018350075572	40.0	35.0	41.0	35.0	41.0
31	38.14153148286581	40.0	35.0	41.0	35.0	41.0
32	38.10365273632384	40.0	35.0	41.0	35.0	41.0
33	38.01091382976208	40.0	35.0	41.0	35.0	41.0
34	37.923197594792356	40.0	35.0	41.0	35.0	41.0
35	37.90142177163987	40.0	35.0	41.0	35.0	41.0
36	37.806259287349924	40.0	35.0	41.0	35.0	41.0
37	37.75674645830264	40.0	35.0	41.0	35.0	41.0
38	37.73229424143381	40.0	35.0	41.0	35.0	41.0
39	37.64886769357247	40.0	35.0	41.0	35.0	41.0
40	37.57966613739003	40.0	35.0	41.0	34.0	41.0
41	37.57848572317195	40.0	35.0	41.0	34.0	41.0
42	37.49500370129882	40.0	35.0	41.0	34.0	41.0
43	37.52244605834411	39.0	35.0	41.0	34.0	41.0
44	37.497284501652395	39.0	35.0	41.0	34.0	41.0
45	37.48843957970706	39.0	35.0	41.0	35.0	41.0
46	37.46963207089033	39.0	35.0	41.0	35.0	41.0
47	37.385157882675195	39.0	35.0	41.0	35.0	41.0
48	37.41305858237527	39.0	35.0	41.0	35.0	41.0
49	37.36264907503906	39.0	35.0	41.0	35.0	41.0
50	37.27696628089493	39.0	35.0	41.0	34.0	41.0
51	37.20526748477193	39.0	35.0	41.0	34.0	41.0
52	36.96200121133416	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	5.0
15	4.0
16	10.0
17	33.0
18	58.0
19	127.0
20	233.0
21	363.0
22	656.0
23	1103.0
24	1867.0
25	3231.0
26	4523.0
27	5455.0
28	5582.0
29	6260.0
30	7325.0
31	9306.0
32	12223.0
33	18000.0
34	52631.0
35	244507.0
36	33607.0
37	50512.0
38	91506.0
39	545196.0
40	5290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.646572342658423	22.6735927334501	26.231295709221598	20.448539214669875
2	24.63800933782218	24.14729168599163	27.714088762056505	23.500610214129686
3	25.587979054468203	25.291966089918827	26.946910461307333	22.173144394305638
4	25.72202609279256	27.20709267070081	24.557253727217006	22.513627509289623
5	24.076539585709167	34.17908466061727	21.175066887107658	20.569308866565905
6	87.6571233178188	2.1087399760279517	7.455798125524048	2.7783385806292027
7	89.26277766561721	1.5980153035519737	4.777039943107308	4.362167087723511
8	79.94168862891887	2.945033439006779	10.445028891956632	6.668249040117715
9	44.910941475826974	16.790164548650708	13.231552162849871	25.067341812672446
10	40.53904370078955	15.74252419485383	28.566842546566342	15.15158955779028
11	38.441762291131255	18.257770453995676	23.171040019497752	20.12942723537532
12	19.386803005418265	35.746634728186436	27.75628538741777	17.110276878977533
13	13.511013864865307	39.23631383376349	30.117477587589825	17.13519471378138
14	13.366781434212369	24.833714376135628	45.02598184453818	16.773522345113832
15	14.879130313000744	19.305865512807223	46.428564932785505	19.38643924140653
16	14.982984938351093	21.47553596080079	28.11923093012639	35.42224817072173
17	18.806508465697963	23.658483795222686	34.17171843937964	23.363289299699712
18	23.73405576866064	27.616509065908584	28.237727056949076	20.411708108481704
19	25.78122868570244	24.34936259451044	25.828154243216257	24.04125447657087
20	18.580701955413446	31.464222900035832	27.98372883575509	21.97134630879563
21	19.57641499653515	23.64447888077089	28.739812334146347	28.03929378854762
22	16.156578581211225	29.836833652536253	25.193295101735703	28.813292664516823
23	17.110549701986333	22.806093774724584	38.602364102312265	21.480992420976815
24	18.061156005652894	22.878028108045186	35.34576678725443	23.715049099047484
25	14.859396115364119	32.73494153402922	29.247354071519645	23.158308279087024
26	16.54426007671783	29.301282086259363	29.993434059588182	24.161023777434625
27	18.424101548361516	26.239662281491505	36.80937128847032	18.526864881676662
28	15.367392557752083	25.11945100735349	33.705009212323596	25.80814722257083
29	17.646010327260292	27.88205679447515	32.43429057833021	22.03764229993434
30	27.81185034021029	25.262046499953623	25.37263075952107	21.55347240031502
31	25.179563010292703	28.84375790049963	26.555045679665774	19.421633409541894
32	23.920212001666037	25.84897973288809	29.19015218067431	21.040656084771562
33	17.84562582869989	25.54905630521256	31.232959929575287	25.37235793651227
34	16.79916770794115	27.472640399267377	31.897738661021048	23.83045323177042
35	24.826348154897993	23.216965225979298	29.62130347558325	22.33538314353946
36	19.27731003788602	28.313480912392897	29.777722000629314	22.631487049091774
37	18.397546775504857	30.29162960820797	28.526100977252018	22.784722639035152
38	16.69122073745878	28.197167369640617	27.75901361750578	27.35259827539482
39	23.391026305594508	23.24006424072447	27.180719779850023	26.188189673830998
40	15.80281807979891	21.345217503596718	31.52042443984889	31.33153997675548
41	20.554758306096502	25.528412697546592	25.151007535371505	28.765821460985403
42	21.606400064022466	22.645128199531836	28.65141767929473	27.097054057150967
43	21.497270860501956	26.449008470245012	29.778267646646917	22.275453022606115
44	16.648478466079915	31.09191043402503	28.333851697050054	23.925759402845
45	20.999096046430836	27.200726800495445	28.511095711767947	23.28908144130577
46	22.450969158268265	23.574181485503097	28.355313773742424	25.61953558248622
47	20.53456940344521	19.848419536310015	34.318224395105915	25.298786665138856
48	19.683907262002847	18.633629619120892	31.360732038697215	30.321731080179042
49	18.908180506977903	24.646103087083286	32.390729837924944	24.054986568013867
50	18.44629115307735	25.58843375948287	29.579106850221986	26.386168237217788
51	19.144445232599804	20.94153039157377	31.44221517732586	28.471809198500566
52	20.854863615777898	19.973645297349798	35.25991848048497	23.91157260638733
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	146.0
1	227.5
2	309.0
3	1705.5
4	3102.0
5	2159.0
6	1216.0
7	1345.0
8	1474.0
9	1537.0
10	1600.0
11	1602.5
12	1605.0
13	1597.5
14	1479.5
15	1369.0
16	1303.0
17	1237.0
18	1364.0
19	1491.0
20	1540.5
21	1590.0
22	2136.0
23	2682.0
24	2887.5
25	3093.0
26	4081.5
27	5070.0
28	6233.5
29	7397.0
30	8616.5
31	9836.0
32	10909.5
33	11983.0
34	14497.0
35	17011.0
36	18933.0
37	20855.0
38	24644.0
39	33834.0
40	39235.0
41	56328.0
42	73421.0
43	97520.5
44	121620.0
45	143889.0
46	166158.0
47	159301.5
48	152445.0
49	131996.5
50	111548.0
51	97834.5
52	84121.0
53	70921.5
54	57722.0
55	50540.0
56	43358.0
57	37953.0
58	32548.0
59	31321.5
60	30095.0
61	27059.0
62	24023.0
63	20120.5
64	12679.5
65	9141.0
66	7502.0
67	5863.0
68	4954.5
69	4046.0
70	2877.5
71	1709.0
72	1447.5
73	1186.0
74	1030.0
75	874.0
76	754.0
77	634.0
78	483.0
79	332.0
80	228.0
81	124.0
82	66.5
83	9.0
84	49.0
85	89.0
86	46.0
87	3.0
88	2.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1099614.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.171498606101043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.49812725208531	13.866935098197528
2	13.580647254558034	5.750453088396507
3	6.11779975024446	3.885689666541177
4	3.350883854258165	2.837729313985329
5	1.9282350624924587	2.041181296889712
6	1.1886818466896876	1.5099705636173
7	0.8422951623710468	1.2482855599244989
8	0.5740270572255634	0.9722410433532238
9	0.42304734337917976	0.8060891616600347
>10	3.980223047013968	21.582945746676483
>50	2.2021204078342254	31.932826410232412
>100	0.30228559289058754	10.151038092343471
>500	0.007320306380541293	1.0274385416824066
>1k	0.0034448500614311975	1.3628375119441256
>5k	8.612125153577994E-4	1.0243389045557694
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	5878	0.5345512152446221	RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTT	5358	0.48726189371906864	TruSeq Adapter, Index 10 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	4187	0.38076997928363954	Illumina PCR Primer Index 2 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1988	0.1807907138323084	TruSeq Adapter, Index 10 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	1856	0.16878650144505253	Illumina PCR Primer Index 2 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1606	0.14605125071161335	No Hit
GCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1454	0.13222821826568232	Illumina PCR Primer Index 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1412	0.12840869614246456	TruSeq Adapter, Index 10 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	1224	0.11131178759091827	TruSeq Adapter, Index 10 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1222	0.11112990558505076	TruSeq Adapter, Index 10 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4956284659889743	0.0
2	0.0	0.0	0.0	1.7163295483687913	0.0
3	0.0	0.0	0.0	2.7294123210508414	0.0
4	0.0	0.0	0.0	3.3129807368767588	0.0
5	0.0	0.0	0.0	6.539203756954713	0.0
6	0.0	0.0	0.0	7.033468107899681	0.0
7	0.0	0.0	0.0	9.103467216677853	0.0
8	0.0	0.0	0.0	11.43892311301966	0.0
9	0.0	0.0	0.0	13.324584808851107	0.0
10	0.0	0.0	0.0	14.588573808627391	0.0
11	0.0	0.0	0.0	15.706966262706732	0.0
12	0.0	0.0	0.0	16.652479870209	0.0
13	0.0	0.0	0.0	16.988415935046298	0.0
14	0.0	0.0	0.0	17.2470521473899	0.0
15	0.0	0.0	0.0	18.28714439794328	0.0
16	0.0	0.0	0.0	19.489475397730477	0.0
17	0.0	0.0	0.0	21.08394400216803	0.0
18	0.0	0.0	0.0	21.755998013848497	0.0
19	0.0	0.0	0.0	22.54863979541912	0.0
20	0.0	0.0	0.0	23.60146378638322	0.0
21	0.0	0.0	0.0	24.425116449954256	0.0
22	0.0	0.0	0.0	25.436653225586433	0.0
23	0.0	0.0	0.0	26.09479326381803	0.0
24	0.0	0.0	0.0	26.6607191250748	0.0
25	0.0	0.0	0.0	27.235739086624942	0.0
26	0.0	0.0	0.0	27.71909051721786	0.0
27	0.0	0.0	0.0	28.23227059677305	0.0
28	9.094100293375675E-5	0.0	0.0	28.738357278099407	0.0
29	9.094100293375675E-5	0.0	0.0	29.45742778829662	0.0
30	1.818820058675135E-4	0.0	0.0	30.083010947477934	0.0
31	1.818820058675135E-4	0.0	0.0	30.579821646505046	0.0
32	1.818820058675135E-4	0.0	0.0	31.078723988599634	0.0
33	1.818820058675135E-4	0.0	0.0	31.59463229824284	0.0
34	1.818820058675135E-4	0.0	0.0	32.316885743542734	0.0
35	2.7282300880127026E-4	0.0	0.0	32.82379089389549	0.0
36	2.7282300880127026E-4	0.0	0.0	33.346519778758726	0.0
37	2.7282300880127026E-4	0.0	0.0	33.83851060463035	0.0
38	2.7282300880127026E-4	0.0	0.0	34.30112748655437	0.0
39	2.7282300880127026E-4	0.0	0.0	34.866871465805275	0.0
40	2.7282300880127026E-4	0.0	0.0	35.416337005531034	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGC	20	6.311333E-4	46.000004	7
ATCTAGT	20	6.311333E-4	46.000004	34
GCGAAAC	20	6.311333E-4	46.000004	8
CTTCACG	40	5.6115823E-9	46.000004	15
CGACCCA	20	6.311333E-4	46.000004	28
TACGGAC	20	6.311333E-4	46.000004	38
AAGCCGT	20	6.311333E-4	46.000004	46
TCGCTAG	20	6.311333E-4	46.000004	10
GCGATAG	45	3.092282E-10	46.000004	8
ACGTCGT	20	6.311333E-4	46.000004	13
CGTGTAT	40	5.6115823E-9	46.000004	21
AACTCGC	20	6.311333E-4	46.000004	36
TTTTGCG	20	6.311333E-4	46.000004	18
CGCGTTG	20	6.311333E-4	46.000004	21
CACGTCG	20	6.311333E-4	46.000004	12
ATCGGGT	20	6.311333E-4	46.000004	3
CACCGCA	20	6.311333E-4	46.000004	18
CAATCGG	35	1.0195072E-7	46.000004	1
TCGGTCG	20	6.311333E-4	46.000004	1
TCGGTAC	45	3.092282E-10	46.000004	35
>>END_MODULE