FastQCFastQC Report
Thu 26 May 2016
SRR1544793_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544793_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences691190
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT54770.7924015104385191RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTT45590.6595870889335783TruSeq Adapter, Index 10 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT31280.4525528436464648Illumina PCR Primer Index 2 (95% over 23bp)
TGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTTG23260.33652107235347734Illumina PCR Primer Index 2 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT13940.20168115858157667Illumina PCR Primer Index 2 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC13730.19864292018113688TruSeq Adapter, Index 10 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA12170.17607314920644107No Hit
CGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC11200.16203938135679047TruSeq Adapter, Index 10 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC10710.1549501584224309Illumina PCR Primer Index 2 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC9370.13556330386724347TruSeq Adapter, Index 10 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT8860.12818472489474675TruSeq Adapter, Index 10 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6950.10055122325265123No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCGG1550.046.01
CGGCCTA253.415733E-546.043
TTGTCGA253.415733E-546.023
TCGGGAC206.3097005E-446.04
CGACATG206.3097005E-446.037
CACGACA206.3097005E-446.024
GTAGCGG1200.046.01
TAACCGG301.8603187E-646.01
ACGTGTA301.8603187E-646.020
ACTACGG405.6061253E-946.01
GTCGTAG253.415733E-546.042
ACTCGGC206.3097005E-446.025
GCGATAG301.8603187E-646.08
ATGGTCG206.3097005E-446.011
TTCGGAC253.415733E-546.013
CCGGTAT206.3097005E-446.042
GCGGACG206.3097005E-446.031
ACGTAAG253.415733E-546.019
GGACCGA1550.046.07
CGGTAGA206.3097005E-446.024
Produced by FastQC (version 0.11.5)