##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544793_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	691190
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9856479405084	34.0	33.0	34.0	31.0	34.0
2	33.298603857115985	34.0	34.0	34.0	31.0	34.0
3	33.38240859966145	34.0	34.0	34.0	31.0	34.0
4	36.61027503291425	37.0	37.0	37.0	35.0	37.0
5	36.59859517643484	37.0	37.0	37.0	35.0	37.0
6	36.574159059014164	37.0	37.0	37.0	35.0	37.0
7	36.46472026505013	37.0	37.0	37.0	35.0	37.0
8	36.50869225538564	37.0	37.0	37.0	35.0	37.0
9	38.34376365398805	39.0	39.0	39.0	37.0	39.0
10	38.20953572823681	39.0	39.0	39.0	35.0	39.0
11	37.49494060967317	39.0	35.0	39.0	35.0	39.0
12	37.426746625385206	39.0	35.0	39.0	35.0	39.0
13	37.3923581070328	39.0	35.0	39.0	35.0	39.0
14	38.63893285493135	41.0	35.0	41.0	35.0	41.0
15	38.70125001808475	41.0	35.0	41.0	35.0	41.0
16	38.72100724836876	41.0	35.0	41.0	35.0	41.0
17	38.806235622621855	41.0	36.0	41.0	35.0	41.0
18	38.79254763523778	41.0	36.0	41.0	35.0	41.0
19	38.71520276624373	41.0	35.0	41.0	35.0	41.0
20	38.65347299584774	41.0	35.0	41.0	35.0	41.0
21	38.602335103227766	41.0	35.0	41.0	35.0	41.0
22	38.57816953370274	41.0	35.0	41.0	35.0	41.0
23	38.576741561654536	41.0	35.0	41.0	35.0	41.0
24	38.53151231933332	41.0	35.0	41.0	35.0	41.0
25	38.50489590416528	40.0	35.0	41.0	35.0	41.0
26	38.45435842532444	40.0	35.0	41.0	35.0	41.0
27	38.48912310652643	40.0	35.0	41.0	35.0	41.0
28	38.552072512623155	40.0	36.0	41.0	35.0	41.0
29	38.565534802297485	40.0	36.0	41.0	35.0	41.0
30	38.50448357181094	40.0	35.0	41.0	35.0	41.0
31	38.448293522765084	40.0	35.0	41.0	35.0	41.0
32	38.43740794861037	40.0	35.0	41.0	35.0	41.0
33	38.37081844355387	40.0	35.0	41.0	35.0	41.0
34	38.29524588029341	40.0	35.0	41.0	35.0	41.0
35	38.25266424572115	40.0	35.0	41.0	35.0	41.0
36	38.21316569973524	40.0	35.0	41.0	35.0	41.0
37	38.1577192957074	40.0	35.0	41.0	35.0	41.0
38	38.149814088745494	40.0	35.0	41.0	35.0	41.0
39	38.117796843125625	40.0	35.0	41.0	35.0	41.0
40	38.09018648996658	40.0	35.0	41.0	35.0	41.0
41	38.072600876748794	40.0	35.0	41.0	35.0	41.0
42	38.02007118158538	40.0	35.0	41.0	35.0	41.0
43	37.95376090510569	40.0	35.0	41.0	35.0	41.0
44	37.83321517961776	40.0	35.0	41.0	35.0	41.0
45	37.83013209103141	40.0	35.0	41.0	35.0	41.0
46	37.802974580072046	40.0	35.0	41.0	35.0	41.0
47	37.744808229285724	40.0	35.0	41.0	35.0	41.0
48	37.735023654856114	40.0	35.0	41.0	35.0	41.0
49	37.69771553407891	40.0	35.0	41.0	35.0	41.0
50	37.62856956842547	39.0	35.0	41.0	35.0	41.0
51	37.56144909503899	39.0	35.0	41.0	35.0	41.0
52	37.171058609065526	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	3.0
15	7.0
16	12.0
17	26.0
18	42.0
19	83.0
20	127.0
21	199.0
22	348.0
23	564.0
24	927.0
25	1588.0
26	2267.0
27	2678.0
28	2655.0
29	2778.0
30	3269.0
31	4288.0
32	5784.0
33	9000.0
34	28245.0
35	151054.0
36	18671.0
37	29051.0
38	55455.0
39	366277.0
40	5787.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.840275466948306	22.35680493062689	25.125218825503843	22.67770077692096
2	24.029282831059476	24.78233192031135	27.788017766460744	23.400367482168434
3	25.565908071586684	25.36089931856653	27.078082726891306	21.995109882955482
4	25.815332976460887	26.980859098077232	24.674836152143406	22.528971773318478
5	24.02002343783909	33.605665591226725	21.389198339096342	20.985112631837847
6	86.0149886427755	2.175957406791186	8.201652222977764	3.6074017274555477
7	87.61657431386449	1.6034664853368827	5.594843675400397	5.185115525398226
8	77.39232338430823	3.1283728063195357	11.861716749374269	7.617587059997974
9	43.99108783402538	16.513115062428565	14.029282831059477	25.466514272486577
10	39.26402291699822	15.674705941926243	29.703265382890375	15.35800575818516
11	37.22840318870354	18.18486957276581	23.512203590908435	21.074523647622218
12	18.97596898103271	34.64025810558602	28.68574487478118	17.698028038600093
13	13.404997178778627	38.15144895036097	30.452408165627396	17.991145705233002
14	13.150797899275164	24.917605868140452	43.94638232613319	17.985213906451193
15	14.410798767343277	19.516052026215657	46.05926011661048	20.013889089830585
16	14.722290542397893	22.274772493815014	27.998813640243636	35.00412332354345
17	18.097484049248397	23.86883490791244	34.22532154689738	23.80835949594178
18	23.088586351075683	27.879164918473936	28.43183495131585	20.600413779134534
19	25.13563564287678	24.145314602352464	25.77178489272125	24.947264862049508
20	18.029340702267106	32.12459671002185	27.752571651788944	22.093490935922105
21	19.22987890449804	23.747305371894846	28.373240353593076	28.649575370014034
22	16.14621160607069	30.14395462897322	24.811990914220402	28.89784285073569
23	16.727238530650038	22.873884170777934	38.19789059448198	22.20098670409005
24	18.486667920542832	22.83511046166756	34.64879410871106	24.029427509078545
25	15.120878484931785	32.46010503624184	29.088383801848984	23.330632676977388
26	16.750821047758212	28.70628915348891	29.679393509744063	24.86349628900881
27	19.239572331775634	25.73228779351553	36.3601903962731	18.66794947843574
28	15.483441600717605	25.085577048279056	33.28375699879918	26.14722435220417
29	17.906075030020688	27.458730595060693	32.403680608805104	22.231513766113515
30	27.678641184044906	25.000940407123945	24.984013078892925	22.336405329938223
31	24.938728858924463	28.80235535815044	26.194823420477725	20.064092362447372
32	23.603929454997903	25.104385190757966	29.46150841302681	21.83017694121732
33	17.602685224033912	24.970702701138617	31.356645784805913	26.069966290021558
34	17.1793573402393	27.02107958737829	32.08307411854917	23.716488953833245
35	24.237763856537274	22.73745279879628	29.92245258177925	23.102330762887195
36	19.236100059317987	28.188631201261593	29.48653771032567	23.08873102909475
37	18.340398443264515	29.949073337287867	29.004181194751084	22.706347024696537
38	16.520927675458267	28.186171674937427	27.00878195575746	28.284118693846843
39	23.650081743080772	22.5380864885198	27.351524182930888	26.46030758546854
40	15.915594843675402	20.548040336231715	31.416108450643094	32.12025636944979
41	20.620813379823204	25.424557646956696	24.702035619728292	29.2525933534918
42	21.304417019922166	21.888048148844746	28.986964510481922	27.82057032075117
43	22.042130239152765	25.737930236259203	29.57334452176681	22.646595002821222
44	16.657069691401784	29.912903832520726	29.0974985170503	24.332527959027182
45	20.670871974420926	26.406487362375035	28.8200060764768	24.10263458672724
46	22.612161634282906	22.42726312591328	28.635686280183453	26.324888959620363
47	20.0280675356993	18.93864205211302	35.19885993720974	25.83443047497794
48	19.64076447865276	17.824331949246954	31.224988787453523	31.309914784646768
49	18.660860255501383	23.59929975838771	33.036502264210995	24.703337721899914
50	18.536871193159623	24.510771278519655	30.17173280863438	26.780624719686337
51	19.057856739825517	20.024739941260723	31.70459642066581	29.21280689824795
52	20.866476656201623	19.2751631244665	35.948147397965826	23.910212821366052
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	81.0
1	130.0
2	179.0
3	840.5
4	1502.0
5	1080.0
6	658.0
7	735.0
8	812.0
9	836.0
10	860.0
11	883.0
12	906.0
13	882.5
14	813.0
15	767.0
16	735.5
17	704.0
18	784.0
19	864.0
20	883.0
21	902.0
22	1271.0
23	1640.0
24	1693.0
25	1746.0
26	2265.0
27	2784.0
28	3454.5
29	4125.0
30	4931.0
31	5737.0
32	6263.0
33	6789.0
34	8345.0
35	9901.0
36	10955.0
37	12009.0
38	14174.0
39	19642.0
40	22945.0
41	33590.5
42	44236.0
43	59908.0
44	75580.0
45	92169.5
46	108759.0
47	104780.5
48	100802.0
49	86622.0
50	72442.0
51	63030.0
52	53618.0
53	45040.5
54	36463.0
55	31682.0
56	26901.0
57	23619.0
58	20337.0
59	19290.5
60	18244.0
61	16303.0
62	14362.0
63	12679.0
64	8332.0
65	5668.0
66	4687.0
67	3706.0
68	3177.5
69	2649.0
70	1901.0
71	1153.0
72	954.5
73	756.0
74	669.0
75	582.0
76	518.5
77	455.0
78	339.0
79	223.0
80	151.5
81	80.0
82	44.0
83	8.0
84	34.5
85	61.0
86	30.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	691190.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.142548267066307
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.57345842357864	16.989689399874244
2	13.417534599387803	6.7470202258028005
3	5.279414261809958	3.9821378369818534
4	2.7100613281693624	2.725513910008321
5	1.4809264588105913	1.8617132485310435
6	0.9331866661673158	1.4077614477776657
7	0.6145947815828995	1.0816735272444584
8	0.4253887578633133	0.855628590147659
9	0.36062344196975293	0.8160293066364088
>10	6.4723447524682705	44.13877535847122
>50	0.5812401843100632	9.459535218852757
>100	0.14025954795168252	5.841044950732588
>500	0.005771997858093931	0.9493929306987152
>1k	0.004617598286475145	2.3492468300444513
>5k	5.771997858093932E-4	0.7948372181957908
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	5477	0.7924015104385191	RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTT	4559	0.6595870889335783	TruSeq Adapter, Index 10 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	3128	0.4525528436464648	Illumina PCR Primer Index 2 (95% over 23bp)
TGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTTG	2326	0.33652107235347734	Illumina PCR Primer Index 2 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	1394	0.20168115858157667	Illumina PCR Primer Index 2 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1373	0.19864292018113688	TruSeq Adapter, Index 10 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1217	0.17607314920644107	No Hit
CGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1120	0.16203938135679047	TruSeq Adapter, Index 10 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1071	0.1549501584224309	Illumina PCR Primer Index 2 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	937	0.13556330386724347	TruSeq Adapter, Index 10 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	886	0.12818472489474675	TruSeq Adapter, Index 10 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	695	0.10055122325265123	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6724634326306804	0.0
2	0.0	0.0	0.0	2.275640561929426	0.0
3	0.0	0.0	0.0	3.478927646522664	0.0
4	0.0	0.0	0.0	4.196241265064598	0.0
5	0.0	0.0	0.0	7.923291714289848	0.0
6	0.0	0.0	0.0	8.510105759631939	0.0
7	0.0	0.0	0.0	10.991478464676861	0.0
8	0.0	0.0	0.0	13.5961168419682	0.0
9	0.0	0.0	0.0	15.75644902269998	0.0
10	0.0	0.0	0.0	17.22319478001707	0.0
11	0.0	0.0	0.0	18.469017202216467	0.0
12	0.0	0.0	0.0	19.56900418119475	0.0
13	0.0	0.0	0.0	19.945311708792083	0.0
14	0.0	0.0	0.0	20.233076288719456	0.0
15	0.0	0.0	0.0	21.377045385494583	0.0
16	0.0	0.0	0.0	22.667139281528957	0.0
17	0.0	0.0	0.0	24.31183900230038	0.0
18	0.0	0.0	0.0	25.027561162632562	0.0
19	0.0	0.0	0.0	25.844557936312736	0.0
20	0.0	0.0	0.0	26.946281051519843	0.0
21	0.0	0.0	0.0	27.80132814421505	0.0
22	0.0	0.0	0.0	28.852992664824434	0.0
23	0.0	0.0	0.0	29.54093664549545	0.0
24	1.4467801906856292E-4	0.0	0.0	30.127171978761268	0.0
25	1.4467801906856292E-4	0.0	0.0	30.708922293435958	0.0
26	1.4467801906856292E-4	0.0	0.0	31.20328708459324	0.0
27	1.4467801906856292E-4	0.0	0.0	31.723983275220995	0.0
28	1.4467801906856292E-4	0.0	0.0	32.23831363300974	0.0
29	1.4467801906856292E-4	0.0	0.0	32.95360175928471	0.0
30	1.4467801906856292E-4	0.0	0.0	33.57340239297444	0.0
31	1.4467801906856292E-4	0.0	0.0	34.074711729047	0.0
32	1.4467801906856292E-4	0.0	0.0	34.56097455113645	0.0
33	1.4467801906856292E-4	0.0	0.0	35.06430937947598	0.0
34	1.4467801906856292E-4	0.0	0.0	35.75861919298601	0.0
35	1.4467801906856292E-4	0.0	0.0	36.25732432471535	0.0
36	1.4467801906856292E-4	0.0	0.0	36.75371460813959	0.0
37	1.4467801906856292E-4	0.0	0.0	37.2331775633328	0.0
38	1.4467801906856292E-4	0.0	0.0	37.71698085909808	0.0
39	1.4467801906856292E-4	0.0	0.0	38.26632329750141	0.0
40	1.4467801906856292E-4	0.0	0.0	38.7942533890826	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGCGG	155	0.0	46.0	1
CGGCCTA	25	3.415733E-5	46.0	43
TTGTCGA	25	3.415733E-5	46.0	23
TCGGGAC	20	6.3097005E-4	46.0	4
CGACATG	20	6.3097005E-4	46.0	37
CACGACA	20	6.3097005E-4	46.0	24
GTAGCGG	120	0.0	46.0	1
TAACCGG	30	1.8603187E-6	46.0	1
ACGTGTA	30	1.8603187E-6	46.0	20
ACTACGG	40	5.6061253E-9	46.0	1
GTCGTAG	25	3.415733E-5	46.0	42
ACTCGGC	20	6.3097005E-4	46.0	25
GCGATAG	30	1.8603187E-6	46.0	8
ATGGTCG	20	6.3097005E-4	46.0	11
TTCGGAC	25	3.415733E-5	46.0	13
CCGGTAT	20	6.3097005E-4	46.0	42
GCGGACG	20	6.3097005E-4	46.0	31
ACGTAAG	25	3.415733E-5	46.0	19
GGACCGA	155	0.0	46.0	7
CGGTAGA	20	6.3097005E-4	46.0	24
>>END_MODULE